Rapid diagnosis of Pseudotuberclosis in yellowtail (Seriola quinqueradiata) by immunofluorescent antibody technique

면역형광항체법(免疫螢光抗體法)에 의(依)한 방어의 유결절증(類結節症) 신속(迅速) 진단(診斷)

Yellow tail (Seriola quinqueradiata) infected by Pasteurella piscicida have been occurred to mass mortality without showing apparent surface lesions in cage culture farms. In this case, it is necessary to consider countermeasure by rapid diagnosis of infected fish. The purpose of the present study was to investigate usefulness of the direct fluorescent antibody technique(FAT) for rapid diagnosis of pseudotuberclosis of cultured yellowtail caused by P. piscicida. Antibody produced by inoculating rabbit with formalin killed pseudotuberclosis bacteria antigen(strain KNP-2). Immunoglobulin-G(IgG) was purified from antisera by DEAE-cellulose column chromatography and conjugate with fluorescein isothiocyanate. Fluorescein-labeled antisera was purified by sephadex G-25 gel column chromatography. The fluorescein/protein molar ratio of labeled antisera was determined as 8.8-9.5. Diagnosis of cultured yellowtail was examined in cage culture farms which located in Tongyung, kyungnam from July to October 1990. The causative bacteria of pseudotuberclosis could be detected within two hours after the specimens were transferred to the laboratory for FAT, and it showed that FAT could be adapted as a rapid and accurate diagnostic method of pseudotuberclosis in yellowtail.

방어의 유결절증(類結節症) 신속진단(迅速診斷)을 위(爲)한 면역형광항체법(免疫螢光抗體法)의 유용성(有用性)을 검토(檢討)하기 위하여 원인균(原因菌)으로 면역(免疫)된 항혈청(抗血淸)에서 면역(免疫)글로브린 G(IgG)를 분리(分離)하여 FITC표식(標識) 항체(抗體)를 정제(精製)하였다. 정제(精製)된 표식항체(標識抗體)는 형광강도(螢光强度)가 1 : 32였으며 이 표식항체(標識抗體)를 이용(利用)하여 1990년(年) 7월(月)부터 10월(月)까지 경남(慶南) 통영군(統營郡) 소재(所在) 방어 양식장(養殖場)을 대상(對象)으로 유결절증진단시험(類結節症診斷試驗)을 실시(實施)한 결과(結果) 직접형광항체법(直接螢光抗體法)으로 2시간내(時間內)에 원인균(原因菌)의 신속진단(迅速診斷)이 가능(可能)하고 균검출면(菌檢出面)에서도 평판도말(平板塗抹) 배양법(培養法)보다 효과적(效科的)인 것으로 나타났다.