Identification and Quantitative Determination of Index Component of Glycyrrhizae Radix from Crude Drug Preparation Containing Bupleuri Radix

시호(柴胡) 함유(含有) 생약제제(生藥製劑)중 감초(甘草) 지표성분(指標成分)의 확인(確認) 및 정량(定量)

As a part of studies on the quality control of crude drug preparation (So-Shi-Ho-Tang), index components of Glycyrrhizae Radix were identified by TLC and quantified by HPLC. Specific red spot (Rf=0.47) was identified in acid hydrolysate of glycosidic fraction on silica gel plate with benzene/ethyl acetate (1 : 1, v/v). The content of glycyrrhizin was determined by quantification of glycyrrhetinic acid by HPLC on ${\mu}-Bondapak\;C_{18}$ column with $MeOH/H_2O/HAc$ (78 : 19 : 3, v/v). Its recovery rate in the extract granules, compared to the content in the Glycyrrhizae Radix, was $83.3{\pm}0.7%$.