Isolation of Hepatic Drug Metabolism Inhibitors from the Seeds of Myristica fragrans

  • Shin, Kuk-Hyun (Natural Products Research Institute, Seoul National University) ;
  • Kim, Ok-Nam (Natural Products Research Institute, Seoul National University) ;
  • Woo, Won-Sick (Natural Products Research Institute, Seoul National University)
  • Published : 1988.09.01

Abstract

The hexane extract from Nutmeg, the seed of Myristica fragrans significantly inhibited hepatic drug-metabolizing enzyme activity. Through systematic fractionation by $SiO_2$ column and vacuum liquid chromatography monitoring by bioassay, three components, myristicin, (I), licarin-B (II) and dehydrodiisoeugenol (III) were isolated as active principles. Compounds II and III, with a single treatment (200mg/kg, i.p.) showed not only a significant prolongation of hexobarbital-induced sleeping time but also a significant inhibition of aminopyrine N-demethylase and hexobarbital hydroxylase activities in mice. Compounds I and II provoked a sleep episode at a subhypnotic dose of HB, suggesting that they possess CNS-depressant properties.

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