Effects of Simulated Acid Rain on the Chlorophyll Contents in the Needles of Pinus koraiensis and Ligustrum obtusifolium Seedlings

인공산성우(人工酸性雨)가 잣나무 및 쥐똥나무 유묘(幼苗)의 엽내(葉內) 엽록소(葉綠素) 함량(含量)에 미치는 영향(影響)

With the purpose of pursuing the increase and decrease of chlorophyll a and chlorophyll b caused by application of the simulated acid rain treatment on Pinus koraiensis seedlings and Ligustrum obtusifolium cuttings, the experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agricultural Junior College, during the growing season of 1985. Pinus koraiensis seeds stratified in cool and moist condition were sown on pots, and in case of Ligustrum obtusifolium, C1/1 cuttings were potted for experimental use in the early spring. The regime of artificial acid rain, based upon precipitation frequency and density, was simulated from the learning of climatological data averaged from 30 years records. The spray of acid water containing pH values of 4.0 and 2.0 was initiated from the 1st of May and ended on the 31st of August. As control, ground water was also treated at the same time. To analyse the chlorophyll content, those leaves looking representative and unaffected by other harmful agents were sampled on the 18th of September, and UV-visible spectrophotometer was used. With decrease in pH values of acid rain, the content of chlorophyll a and chlorophyll b decreased in both species. The decrease in chlorophyll a could be confirmed through statistical significance, but not in chlorophyll b. And when we discussed the chlorophyll decrease index which was explained in detail in the paper, an attention might be given to similarly decreasing values in both chlorophyll a and chlorophyll b, according as pH levels of acid rain decreased. The ratios of chlorophyll a to chlorophyll b in both species were not affected by different pH leaves of acid rain.

잣나무 유묘(幼苗)와 쥐똥나무 삽목묘(揷木苗)를 인공산성우(人工酸性雨)로 처리(處理)하고 엽내(葉內) 엽록소(葉綠素) a와 b의 함량(含量)을 분석(分析)하여 양(量)의 증감(增減)에 따른 산도(酸度) 수준(水準)의 영향(影響)을 논의(論議)하였다. 처리(處理)된 시험수종(試驗樹種)은 1985년(年) 봄에 pot에 재배(栽培)되고 해당지역(該當地域)의 30년간(年間)의 강우(降雨) 양식(樣式)을 모의(模擬)해서 산성우(酸性雨)(pH 수준(水準) 6.5, 4.0 및 2.0)를 5월(月)부터 8월(月)까지 4개월간(個月間)에 걸쳐 살포(撒布)하고, 동년(同年) 9월(月) 18일(日) 성숙(成熟)된 엽(葉)을 채취(採取)하여 엽중(葉中)의 엽록소(葉綠素)를 분석(分析)하였다. 분석(分析) 결과(結果)에 의(依)하면, 양(兩) 수종(樹種) 공통적(共通的)으로 산성우(酸性雨)의 산도(酸度)가 강(强)해짐에 따라 엽록소(葉綠素) a와 b의 함량(含量)은 감소(減少)하는 경향(傾向)을 보였고. 엽록소(葉綠素) a에 있어서는 감소(減少) 경향(傾向)이 통계적(統計的)으로 유의(有意)했고, 엽록소(葉綠素) b의 경우(境遇)는 유의(有意)하지 않았으나 감소(減少) 경향(傾向)이 있는 것으로 받아들일 수 있었다. 산성우(酸性雨)의 pH 수준(水準)에 따른 엽록소(葉綠素) a와 b에 대한 감소(減少) 효과(効果) 차이(差異)에 있어서는 양(兩) 수종(樹種) 모두 pH 수준별(水準別)로 감소(減少) 지수(指數)가 유사(類似)하였으며, 따라서 엽록소(葉綠素) a 대(對) b의 함량비(含量比)는 산성우(酸性雨)의 pH 수준(水準) 여하(如何)에 불구(不拘)하고 일정(一定)한 값을 유지(維持)하고 있었다.