Experiment of the formulation for the viral pesticide of nuclear polyhedrosis virus of the fall webworm, Hyphantria cunea Drury.

흰불나방 핵다각체병 바이러스의 제제화에 관한 시험

The intent of this research is to acquire some basic informations about formulation of the viral pesticide, Hyphantria cunea nuclear polyhedrosis virus and its virulence under field condition. The nuclear polyhedrosis virus was formulated as wettable powder using spreader, sticker and U.V. protector. The formulated product and aqueous virus were diluted with water at the concentration of 1${\times}$106PIB/ml and sprayed on mulberry leaves in the field. The leaves were fed with 3rd instar larvae of H. cunea to determine the inactivation period of the viral pesticides. The aqueous virus was completely inactivated on 5th day after spray, while the formulated one showed a spare mortality to the larvae even on 20th day after spray. In field application test, The fromulated and aqueous virus were sprayed on individual mulberry tree and 3rd instar laevae of H. cunea were fed on the trees. The mortality of the larvae one day after spray of the formulated and aqueous virus were about 50% and 40%, respectively. The formulated virus exhibited a persistent virulence to the larvae up to 9th day after spray, which the mortality was approximately 30%. The residual virulence of the formulated and aqueous virus was extended up to 4th day and 2nd day after spray, respectively.

흰불나방의 미생물적 방제를 위한 핵다각체병 바이러스의 제제화와 그 실용화를 위한 야외 살포 시험을 통해 얻어진 제제의 불활화 정도 및 살포효과는 다음과 같다. 흰불나방 핵다각체병 바이러스의 불활화 조사에서 부유액의 경우 야외 살포후 5일이 경과한 후로는 완전히 불활화 되었으며, 제제는 살포 후 20일이 지나서 완전히 불활화 되어, 흰불나방 핵다각체병 바이러스를 제제화 했을 때 부유액에 비해 약 10-15일 정도 활성을 보유하여 제제의 안전성을 확인하였다. 또 제제의 야회 살포효과 시험에서 부유액의 경우는 2일이 경과하면서 병원성을 보이지 않은 반면에 제제는 접종당일 살포하였을 때 약 50%정도의 폐사율을 나타내었으며, 살포 후 9일까지는 약 30% 정도의 폐사율을 유지하면서 비교적 안전성을 보이다가 살포 후 14일이 경과하면서 병원성을 보이지 않았다. 제제의 경우 야외에서 약 10일 이상 감염력의 지속에 의한 살포효과를 나타내었다.