Inhibitory Effect of High Temperature on the Multiplication of Flacherie Virus in the Silkworm, Bombyx mori L.

바이러스성 건화병의 고온에 의한 발병역제 효과

  • Published : 1987.10.01

Abstract

The silkworms were inoculated with flacherie virus and reserved at high temperature (37$^{\circ}C$) to find out the effect of high temperature on the multiplication of the virus in silkworms. The obtained results are summarized as follows. 1. It was shown that the inhibitory effect of high temperature treatment on the virus multiplication was achieved at high level at the newly molted stages in 5th instar larvae and both in 4th and in 5th larvae. 2. Twelve hours treatment and 24 hours treatment showed high inhibitory effect on the multiplication of flacherie virus. 3. The silkworms were inoculated with flacherie virus at the newly molted stage in 2nd instar larvae and followed by have temperature (37$^{\circ}C$) treatment at the newly molted stages in 5th instar and both in 4th and in 5th instar for 12 and 24 hours respectively. The increase in the survival rate with high temperature treatment ranged from 33.8 to 39.5 percent in index number and cocoon productive was 45.8 to 54.2 percent compared to non-treatment. 5. In the inoculation of the virus to the 4th instar larvae, the high temperature treatment at the newly molted stage in 5th instar brought increases in the survial rate from 49.7 to 68.9 percent in index number and in cocoon production from 44.3 to 69.0 percent.

고도(37$^{\circ}C$)처리에 의한 바이러스성 건연병의 발병억제 효과를 검정한 결과는 다음과 같다. 1. 고온처리 시기는 5령기잠 또는 4.5령기잠이 가장 알맞았다. 2. 고온처리 기간은 12-24시간이 적절했다. 3. 2령기잠에 바이러스를 접종하고 5령기잠 또는 4,5령기잠에 각각 12 및 24시간 고온처리 했을 때, 생존율은 고온 무처리 대비 지수로 33.8-39.5%가 높았고, 1만두 수견량 24.2-32.0%은 높았다. 4. 3령기잠에 바이러스를 접종하고 위와 같은 방법으로 고온처리 했을 때, 생존율은 무처리 대비 지수로 53.6-70.0%, 1만두 수견량은 45.8-54.2%가 높았다. 5. 4령기잠에 바이러스 접종 후 5령기잠에 12 및 24시간 고온처리 했을 때는 무처리 대비 지수로 생존율은 49.7-68.9%, 1만기 수견량은 44.3-69.0%이 높았다.

Keywords