Synthesis of Trehalose by Pleurotus spp. -Cultural conditions­-

느타리버섯균의 Trehalose 합성(合成)(I) -배양(培養) 조건(條件)-

Nutritional characteristics and cultural conditions for trehalose synthesis and mycelial yield of Pleurotus sajor-caju and Pleurotus ostreatus 201 in submerged culture were investigated. The results were as follows: Among the carbon sources, glucose was most excellent for trehalose synthesis and mycelial yield. The optimal concentration of glucose was 1%. Among the nitrogen sources, peptone was most excellent for trehalose synthesis and mycelial yield. The optimal concentration of peptone was 0.05%. The optimal concentration of $KH_2PO_4$ and $MgSO_47H_2O$ for trehalose synthesis and mycelial yield was 0.1%,0.04% and 0.2%,0.04-0.08%, respective­ly. The optimal temperature and pH for trehalose synthesis were $25^{\circ}C$ and pH 5.5. but optimal temperature and pH for mycelial yield were $30^{\circ}C$ and pH 5.5. The maximum yield of trehalose was obtanined after 10 day cultivation.

합성배지(合成培地)에서 P. sajor-caju와 P. osreatus 201의 배양조건(培養條件)을 달리하여 배양(培養)할 때 trehalose 합성(合成)과 균체양(菌體量)의 변화(變化)를 조사(調査)한 결과(結果)는 다음과 같다. 1. Trehalose 합성(合成)에 적합(適合)한 탄소원(炭素源)은 양균주(兩菌株) 모두 glucose였고 그 다음이 maltose였으며 glucose 최적농도는 1%였다. 균체수률(菌體收率)이 높은 탄소원(炭素源)은 glucose, maltose 순이었다. 2. Trehalose 합성(合成)에 적합(適合)한 탄소원(炭素源)은 양균주(兩菌株) 모두 peptone 이었고 그 농도(濃度)는 0.05%였으며 균체수률(菌體收率)은 peptone, casamino acid, $(NH_4)_2HPO_4$ 순으로 높았다. 3. Trehalose 합성(合成)에 적합(適合)한 $NH_2HPO_4$와 $MgSO_47H_2O$의 농도(濃度)는 양균주(兩菌株) 모두 0.1%, 0.04% 였고 균체수률(菌體收率)은 $KH_2PO_4$ 0.2%, $MgSO_47H_2O$ $0.04{\sim}0.08%$가 높았다. 4. Trehalose 합성(合成)에 적합(適合)한 온도(溫度)와 pH는 $25^{\circ}C$, pH5.5였고 균체수률(菌體收率)은 $30^{\circ}C$, pH5.5에서 높았으며 trehalose 합성(合成)은 10일간(日間) 배양(培養)했을 때 균체수률(菌體收率)은 14일간(日間) 배양(培養)했을 때가 가장 양호(良好)하였다.