Changes in Amounts of Polysaccharides and Polyphosphates under Catabolic Repression and Derepression in Yeast (V)

Catabolic Repression 및 Derepression에 의한 효모 세포의 다당류 함량 변화와 무기 폴리 인산(제 5 보)

The present study was designed to investigate biosynthetic patterns of polysaccharides under catabolic repression and derepression in Saccharomyces uvarum. Correlation coefficients between polysaccharide synthesis and polyphosphate accumulation were examined, according to the culture phase and under various phosphate concentrations (free, limited, sufficient). During catabolic derepression, biosynthesis of glycogen was enhanced. rapidly and highly in the cells grown on minimal medium, compared with those grown on the complete medium. Acid soluble glycogen type was the main component of total glycogen and alkali soluble glycogen was synthesized in small amount, after 24 hr culture, at the time of almost exhaustion of sugar in the medium. Total glycogen was accumulated highly in proportion to the amount of phosphate added to the medium. It could be postulated that type 'C' isoenzyme among ALPase was directly or indirectly correlated with the glucan synthesis. Mannan synthesis indicated maximal amount at the early exponential phase and stationary phase, and also acid soluble sugars at the stationary phase. Correlation coefficient between the mannan synthesis and poly-p-'C' accumulation, and also between mannan synthesis and phospholipid content indicated 0.866 and 0.726, respectively.

본 연구는, catabolic repression시킨 효모세포를 완전배지와 최소배지에서 derepression시켜, 배양시기 및 인산 첨가농도(free, limited, sufficient)에 따른 5종의 다당류 합성변화를 조사하였다. 그리고 다당류 합성과 무기폴리인산 축적량 및 인지질 합성 사이의 상관지수를 구하여 합성시 관련되는 유의한 정도를 검정하였다. 그 결과, 최소배지에서 catabolic derepression시킨 효모세포가, 완전배지에서 derepression시킨 세포에 비하여, glycogen의 합성이 발리 그리고 많이 일어났고, acid soluble glycogen type이 주된 함량을 나타내었으며, alkali soluble glycogen은 당이 많이 소모된 24시간 배양 후에 소량 나타났다. 무기인산 첨가정도에 따라 total glycogen합성이 일정한 비율로 빨리 그리고 높게 일어났다. Glucan의 합성에는 ALPase 중 ALPase "C"가 관련할 것으로 추정되었다. Mannan은 ezponential phase초기와 정체기때, acid soluble 분획은 정체기때 최대함량을 나타내었다. Mannan 합성과 poly-P "C"축적량 사이의 상관지수는 0.866, mannan합성과 인지질 사이의 상관지수는 0.726으로 나타나 매우 유의하였다.