Korean Journal of Microbiology (미생물학회지)
- Volume 22 Issue 4
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- Pages.249-255
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- 1984
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Isolation and characterization of corynebacteria-E. coli shuttle vector pKU6 from coryneform bacteria
Corynebacteria-E. coli shuttle vector pKU6의 분리 및 확인
Abstract
To develop the host-vector system for industrial Coryneform bacteria that seemed to be the most suitable microorganisms for molecular breeding of genes involved in the production of amion acids, nucleotides, and other products of industrial interest, broad host range E. coli plasmid R 1162 DNA was transformed into Brevibacterium ammoniagenes and the plasmids pKU6 isolated from a transformant was physically characterized. All other plasmids from the transformed cells except pKU6 exsisted as multimeric forms in Brevibacterium ammoniagenes. The plasmid DNA was retransformed into Corynebacterium glutamicum with a high frequency (
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