한국균학회지 (The Korean Journal of Mycology)
- 제11권1호
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- Pages.23-26
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- 1983
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- 0253-651X(pISSN)
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- 2383-5249(eISSN)
Aspergillus ustus가 생산하는 Exo-dextranase의 정제에 관한 연구
Purification of Exo-dextranase from Aspergillus ustus
- Lee, Kon-Joo (Molecular Microbiology Laboratory, Department of Biology, Kon Kuk University) ;
- Lee, Hyung-Hoan (Molecular Microbiology Laboratory, Department of Biology, Kon Kuk University)
- 발행 : 1983.04.30
초록
Aspergillus ustus의 dextranase을 정제하기 위하여 냉각 Aceton에 침전시키고, DEAE-cellulose, Bio-Gel P-150 및 Sephadex G-200을 사용하며 순수정제하였다. 처음 배양액 10l에 대한 총 효소량은 1,340kunits 단백질 양은 38,500mg였으며, 최종정제 단계인 Sephadex G-200에서는 총 효소 활성량은 421 kunits, 단백질량은 172mg이었으며 비활성은 약 70배로 증가했다.
Aspergillus ustus was cultured in the salts media contained dextran (2%). Then the cultured liquid media were filtrated and concentrated up to 10 folds by evaporation, and then purified by means of acetone precipitation, of a repeated chromatography on the columns of DEAE-Ccellulose, Biogel P-150, and Sephadex G-200. Total proteins in the initial culture filtrate were 38,500mg, but the final amounts of proteins were 172mg. The specific activity of the protein in the culture filtrate was