미생물학회지 (Korean Journal of Microbiology)

  • 제20권4호
  • /
  • Pages.189-194
  • /
  • 1982
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Aspergillus tubingensis의 acid protease에 관한 연구 II

Studies on acid protease produced from Aspergillus tubingensis II

  • 정윤수 (건국대학교 축산가공학과) ;
  • 고동성 (충남대학교 화학과) ;
  • 조영 (충남대학교 화학과) ;
  • 이금수 (충남대학교 생물학과)
  • Chung, Yun-Su (Dept. of Animal Science, Kon Kuk University) ;
  • Ko, Dong-Sung (Dept. of Chemistry, Chung Nam National University) ;
  • Cho, Young (Dept. of Chemistry, Chung Nam National University) ;
  • Lee, Keum-Soo (Dept. of Biology, Chung Nam National University)
  • 발행 : 1982.12.01
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초록

Acid protease produced from Aspergillus tubingensis was pruified by ethanol fractionation, dialysis, and DEAE cellulose column chromatography. As a result of purification its specific activity increased to 5.4 times, and percent recovery was 39. The kinetic constants of the enzyme were studied. Km and Vmax was $1.5{\times}10^{-7}M\;and\;0.11{\Delta}O.D/min$ , respectively, when casein was used as substrate. The order of Km value of several proteins is : casein

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