Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Purification and Serology of Cucumber Mosaic Virus

오이모자익 바이러스의 순화와 항혈청 제조

  • Lee S. H. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD) ;
  • Lee K. W. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD) ;
  • Chung B. J. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD)
  • 이순형 (농촌진흥청 농기연 병리과) ;
  • 이기운 (농촌진흥청 농기연 병리과) ;
  • 정봉조 (농촌진흥청 농기연 병리과)
  • Published : 1978.03.01
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Abstract

Purely isolated cucumber mosaic virus (CMV) was multiplied in Nicotiana tabacum, Ky-57 and the virus was purified by the modified method that was developed through this study. The concentration of purified CMV was 24.25 mg/ml. The purified virus, mixed with acomplet adjuvant (1: 1) was injected into rabbits intramuscularly. Two injections at 10 day interval was enough to produce a good quality antiserum. The titer of the antiserum was 1/1280 when determined by agar gell-diffusion test. The produced antisera will be used to faciliate the detection of CMV infected vegetables and other crops.

순수분리된 오이모자익 바이러스(CMV)를 함배 (KY-57)에서 증식하여 개량된 순화방법으로 순화하였다. 순화된 바이러스를 Complete adjuvant와 1: 1로 섞어 토끼에 10일 간격으로 2회 근육주사하여 높은 역가의 CMV 항혈청을 제조하였다. 한천내확산법에 의한 항혈청의 역가는 1/1280이었다. 제조된 CMV 항혈청은 앞으로 채소작물이나 그밖에 식량작물에 이병된 CMV를 분리동정하는데 이용될 것이다.

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