벼의 약배양에 관한 연구 2. 분화배지에 이식된 Haploid Callus의 발생 및 분화

Studies on the Anther Culture of Rice 2. Histological observation of haploid callus inoculated on differentiation medium

Histological observation of micropore-originated haploid rice callus was reported previously. Present study was attempted to clarify the growth or development of the calli when they were transferred to differentiation media prepared exclusively for differentiation of plantlets. When the callus was transferred to differentiation medium, the cells and tissues became radially elongated. Meristematic tissues were present but few in number, and their structures were quite different from those grown in the propagaton medium. Differentiation of tracheid, chloroplast, and epidermis-like cell layer, and formation of gap in the callus tissue were more conspicuous in differentiation media. Approximately ten days after transfer of callus to differentiation medium, plantlet was formed.