한국농업기계학회:학술대회논문집 (Proceedings of the Korean Society for Agricultural Machinery Conference)
- 한국농업기계학회 2017년도 춘계공동학술대회
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- Pages.107-107
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- 2017
Development of a cell-laden thermosensitive chitosan bioink for 3D bioprinting
- Ku, Jongbeom (Department of Biosystems Engineering, Seoul National University) ;
- Seonwoo, Hoon (International Dental Stem Cell Bank Co. Ltd) ;
- Jang, Kyoung-Je (Department of Biosystems Engineering, Seoul National University) ;
- Park, Sangbae (Department of Biosystems Engineering, Seoul National University) ;
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Chung, Jong Hoon
(Department of Biosystems Engineering, Seoul National University)
- 발행 : 2017.04.06
초록
3D bioprinting is a technology to produce complex tissue constructs through printing living cells with hydrogel in a layer-by-layer process. To produce more stable 3D cell-laden structures, various materials have been developed such as alginate, fibrin and gelatin. However, most of these hydrogels are chemically bound using crosslinkers which can cause some problems in cytotoxicity and cell viability. On the other hand, thermosensitive hydrogels are physically cross-linked by non-covalent interaction without crosslinker, facilitating stable cytotoxicity and cell viability. The examples of currently reported thermosensitive hydrogels are poly(ethylene glycol)/poly(propylene glycol)/poly(ethylene glycol) (PEG-PPG-PEG) and poly(ethylene glycol)/poly(lactic acid-co-glycolic acid) (PEG/PLGA). Chitosan, which have been widely used in tissue engineering due to its biocompatibility and osteoconductivity, can be used as thermosensitive hydrogels. However, despite the many advantages, chitosan hydrogel has not yet been used as a bioink. The purpose of this study was to develop a bioink by chitosan hydrogel for 3D bioprinting and to evaluate the suitability and potential ability of the developed chitosan hydrogel as a bioink. To prepare the chitosan hydrogel solution,