한국생물공학회:학술대회논문집

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Evaluation of alginate microspheres prepared by emulsion method for protein delivery system

  • Park, Ji-Hyun (Department of Material & Biochemical Engineering, Chonnam National University) ;
  • Lee, Chang-Moon (Interdisciplinary Program of Biomedical Engineering, Chonnam National University) ;
  • Kim, Gwang-Yun (Ecobio Inc., Business Incubation Center, Chonnam National University) ;
  • Rhee, Joon-Haeng (Department of Microbiology, Connam National University Medical School) ;
  • Lee, Ki-Young (Faculty of Applied Chemical Engineering and The Research Institute for Catalysis, Chonnam National University)
  • Published : 2005.04.15
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of the study is to research the proper conditions to prepare the calcium-alginate microspheres using a emulsion method. The calcium-alginate microspheres were prepared at distinct concentrations (alginate; 1%, 1.5%, 2% (w/v), calcium chloride; 2%, 4%, 8%, 10%(w/v)). The shape of the microspheres prepared was spherical. With increasing alginate and calcium chloride concentration the mean size of the microspheres decreased gradually. In release test, the amount of ovalbumin released from the calcium-alginate mcirospheres was decreased by the increasing of alginate and calcium chloride concentration. In this study the best result was obtained at a 2% of alginate concentration and 10% of calcium chloride concentration.

본 연구에서는 유화법을 이용한 alginate 미세입자 제조에 있어서 적절한 alginate 농도와 가교제인 calcium chloride의 농도를 조사하였다. 유화법을 통해 calcium-alginate microsphere는 구형으로 제조되었으며 입자의 크기는 alginate와 가교제의 농도가 증가함에 따라 감소하는 경향을 보였다. 약물방출 경향은 alginate의 농도가 2%(w/v)일 때와 calcium chloride의 농도가 10%(w/v)일 때 가장 좋은 결과를 얻을 수 있었다.

Keywords