Release proporties of ovalbumin from alginate microspheres prepared using the nozzle in spray dryer system

The spray method was chiefly used to prepare alginate microspheres. Additionally due to formation at mild conditions, the alginate microspheres were coated with chitosan. The particle size of alginate microspheres increased when the sodium alginate increased. Release pattern of OVA in alginate microspheres was evaluated at PBS buffer(pH 7.4) and HCl buffer(pH 1.2). Release rate of OVA from chitosan/alginate microsphere was also lower than that with the concentration of alginate in the microspheres, the amount of OVA released from alginate microspheres increased from alginate micorsphere. Therefore, the alginate microspheres can be prepared by spray rozzle for a protein drug delivery. OVA release from the alginate microspheres was controlled by a coating with chitosan.

분무법을 이용한 alginate microsphere를 제조한 결과 구형을 형성하는 것을 관찰하였고, 이러한 alginate microsphere에서 alginate 농도가 증가할수록 크기는 작아지고, chitosan/alginate microsphere에서 chitosan의 농도가 증가할수록 그 크기가 증가하는 것을 확인하였다. OVA의 방출정도는 alginate microsphere에서 alginate 농도가 증가할수록 잘 이루어지지 않았고, HCl buffer에서보다 PBS buffer에서 방출이 잘되는 것을 확인하였다. Chitosan/alginate microspheres에서는 chitosan의 농도가 증가할수록 방출이 잘되지 않았고, 이는 alginate microsphere에서와 마찬가지로 PBS buffer에서 방출이 잘 이루어지는 것을 확인하였다.