Proceedings of the Korea Society of Poultry Science Conference (한국가금학회:학술대회논문집)

The Korean Society of Poultry Science (한국가금학회)
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Differential protein expression in avian liver in response to invasion by Salmonella gallinarum

  • Lee, Gang-Deog (Genetic Information Research Center. Hankyong National University) ;
  • Cho, In-Hee (Genetic Information Research Center. Hankyong National University) ;
  • So, Hyun-Kyung (Genetic Information Research Center. Hankyong National University) ;
  • Koo, Yong-bum (School of Biotechnology and Biomedical Science. Inje University) ;
  • Lee, Jun-heon (Division of Animal Science and Resources. Chungnam National University) ;
  • Choi, Kang-Duk (Genetic Information Research Center. Hankyong National University, The Graduate School of Bio &)
  • Published : 2004.11.01
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Abstract

Salmonella gallinarum is a pathogen that is capable of causing disease in Korean native chicken. Although Salmonella gallinarum is important world-wide pathogens of poultry, little is understood of the mechanisms of pathogenesis of Salmonella gallinarum in the chicken. This study was to investigate chicken liver proteins affected by infection of Salmonella gallinarum in Korean native chicken. The differentially expressed proteins of chicken livers were identified by using 2-dimensional electro- phoresis (2D-E) and mass spectrometry (MS). We detected more than 300 protein spots on silver stained 2D gels using pH 3∼10 gradients. Three differentially expressed protein spots were analyzed by MALDI-TOF MS and MS/MS. The obtained MS and MS/MS data were searched against a protein database using the Mascot search engine. Further researches on the identified proteins can give valuable information of mechanism of pathogenesis in chicken.

본 연구는 proteomics의 방법을 이용하여 가금의 질병과 관련된 단백질을 찾고자 수행하였다. 가금티푸스에 감염된 재래계와 대조구와의 비교에서 질병과 관련된 후보 단백질이 이 연구를 통하여 찾아졌다. 이 단백질들은 질병을 조절하고 모니터링하는 가금의 질병 단백질 마커로 중요하게 이용이 될 수 있을 것으로 추정된다.

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