한국생물공학회:학술대회논문집
- 한국생물공학회 2003년도 생물공학의 동향(XII)
- /
- Pages.635-638
- /
- 2003
Detection of viability Change of Escherichia coli O157:H7 using Surface Plasmon Resonance
- Park, Gwang-Won (Department of Chemical Engineering, Sogang University) ;
- Lee, U-Chang (Department of Chemical Engineering, Sogang University) ;
- Lee, Won-Hong (Department of Chemical Engineering, Sogang University) ;
-
Choe, Jeong-U
(Department of Chemical Engineering, Sogang University)
- 발행 : 2003.04.11
초록
For the acute assessment on biological toxicity of wastewater, surface plasmon resonance(SPR) based cell viability detection was performed using gold surface-confined cell as a result of adhesion-modifying chemicals. Escherichia coli O157:H7 (E. coli O157:H7) was investigated after exposure to EDTA. Cells were immobilized on gold coated slide glass for SPR analysis by the method of cross-linking carboxyl group on the bacterial surface with amine group of poly-L-lysine that had been coupled to the gold surface modified by a self-assembled monolayer of 11-mercaptounde canoic acid (11-(MUA)). Reflective intensity of each flow step was changed with respect to confect of ethylenediaminetetraacetic acid (EDTA) disodium salt and phosphate-buffered saline (PBS) solution. The proposed detection technique can be used for biological toxicity test.
키워드