한국생물공학회:학술대회논문집
- 2003.04a
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- Pages.87-90
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- 2003
유전자 재조합 대장균을 이용한 5-Aminolevulinic acid(ALA)의 대량 생산 기술 및 모니터링 기술 개발
- Jeong, Sang-Yun ;
- Lee, Jong-Il ;
- Scheper, Th. (University of hanover, Institute for Technical Chemistry)
- Published : 2003.04.11
Abstract
ALA is an essential intermediate in the tetrapyrrole biosynthesis pathway and has applications as a biodegradable herbicide and insecticide as well as medical applications including photodynamic therapy of cancers. For the development of mass production process of ALA it is necessary to on-line monitor some metabolites such as glycine, succinate, LA and ALA. In this work, medium compositions, pH conditions, induction and addition of LA were investigated to increase the production of ALA by recombinant E. coli. A 2-dimensional fluorescence sensor was used to monitor the processe and the fluorescence spectra were correlated with on- and off-line data like ALAS, ALAD, cell mass etc.
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