유전자 재조합 대장균을 이용한 5-Aminolevulinic acid(ALA)의 대량 생산 기술 및 모니터링 기술 개발

  • 정상윤 (전남대학교, 물질 생물화학공학과, 생물공정기술연구실) ;
  • 이종일 (전남대학교, 응용화학공학부, 생물공정기술연구실) ;
  • Published : 2003.04.11

Abstract

ALA is an essential intermediate in the tetrapyrrole biosynthesis pathway and has applications as a biodegradable herbicide and insecticide as well as medical applications including photodynamic therapy of cancers. For the development of mass production process of ALA it is necessary to on-line monitor some metabolites such as glycine, succinate, LA and ALA. In this work, medium compositions, pH conditions, induction and addition of LA were investigated to increase the production of ALA by recombinant E. coli. A 2-dimensional fluorescence sensor was used to monitor the processe and the fluorescence spectra were correlated with on- and off-line data like ALAS, ALAD, cell mass etc.

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