Proceedings of the Korean Society of Fisheries Technology Conference (한국어업기술학회:학술대회논문집)

The Korean Society of Fisheries and Ocean Technology (한국수산해양기술학회)
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Flow cytometry As a Tool for Monitoring Immune Parameters of the Manila clam Ruditapes philippinarum

  • Park, Kyung-Il (School of Applied Marine Science, Cheju National University) ;
  • Philippe Soudant (School of Applied Marine Science, Cheju National University) ;
  • Park, Kwang-Sik (School of Applied Marine Science, Cheju National University)
  • Published : 2003.05.01
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Abstract

Hemocytes in marine bivalves play important immunological roles in discrimination, opsonization and phagocytosis of foreign materials as a defense mechanism. In this study we report the flow cytometric implications to investigate the immune parameters such as the compositional and the functional characteristics of hemocytes isolated from the Manila clams, Ruditapes philippinarum. Heterogeneity of the hemocytic cell population was determined by the forward scatter (FSC) and side scatter (SSC) cytometric profile which showed three populations: granulocytes, hyalinocytes and small agranular cells. In addition, phagocytosis rate was measured after adding fluorescent-labeled particles. The data were initially analysed for two-parameters: FSC and SSC, then the fluorescent (FL 1) frequency distribution histogram of the hemocyte population was subsequently obtained.

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