Proceedings of the Korean Society of Veterinary Pathology Conference (한국수의병리학회:학술대회논문집)
- 2003.10a
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- Pages.27-27
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- 2003
Differentiation between Porcine Epidemic Diarrhea Virus and Transmissible Gastroenteritis Virus in Formalin-fixed Paraffin-embedded Tissues by Multiplex RT-nested PCR and Comparison with in situ Hybridization
- Jung, Kwon-il (Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
- Kim, Jung-hyun (Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University) ;
- Chae, Chan-hee (Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
- Published : 2003.10.01
Abstract
Porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) infections are considered difficult to distinguish clinically and histopathologically. Prompt differentiation between PEDV- and TGEV-associated enteritis would greatly facilitate the management of disease in countries where PEDV and TGEV are epizootic. Rapid differential diagnosis and treatment are crucial to reducing mortality and morbidity from PEDV- and TGEV-induced enteritis in piglets. The objective for this study was to develop a protocol to differentiate between PEDV and TGEV directly from formalin-fixed, paraffin-embedded tissue, using a multiplex reverse transcription-nested polymerase chain reaction (RT-nPCR) assay. (omitted)
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