한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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- Pages.75.2-75
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- 2003
Localization of Single Chain Fv Antibodies (scFv) in Transgenic Tobacco Ptants Showing Resistance against Tomato Bushy Stunt Virus
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Jeun, Y.C.
(Dept. of plant resource science, Cheju National University) ;
- Boonrod, K. (Centrum Gruene gentechnik, SLFA) ;
- Nagy, P. (University of Kentucky, Dept. of Plant Pathology) ;
- Conrad, U. (Institut of Plant Genetics and Crop Research, Dept. Of Molekular Genetics, Phytoantibodies.) ;
- Krczal, G. (Centrum Gruene gentechnik, SLFA)
- 발행 : 2003.10.01
초록
To develop an effective protection strategy against tomato bushy stunt virus (TBSV), tobacco plants expressing single-chain Fv antibodies (scFv), were established. A previous had shown that the replication activity of viral replicase was inhibited by the selected scFvs. Moreover, no systemic symptom was found after virus inoculation on leaves of wt N. benthamiana infiltrated with an Agrobacterium suspension resulting i3l expression of the scFvs. However, control plants showed systemic symptoms. In this study the localization of the scFvs within two transgenic plant lines, (CP28H3, CP-P55) was demonstrated using immunogold labelling. The gold particles, indicating the presence of scFv, were mostly found In the cytoplasm of the plant cells including chloroplasts and in the cell walls. However, they were hardly found in the vacuole, nucleoplasm and intercellular spaces. Gold particles often accumulated in either the cytosol or chloroplasts showing a specific labeling, There was no difference in type of gold labeling between both transgenic lines. The localization of the scFv in the cytoplasm further conforms the inhibition of the RNA-dependent RNA polymerase (RdRp) by the selected scFv because it is known that the RdRp is localized to membraneous cytosolic structures.
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