한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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- Pages.75.1-75
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- 2003
Coat Protein Gene-Mediated Resistance to Barely Yellow Mosaic Virus-HN and Barely Mild Mosaic Virus-Kor in Transgenic Barely
- Lee, Kui-Jae (Faculty of Bioresource Science, Chonbuk National University) ;
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Kim, Hyung-Moo
(Faculty of Bioresource Science, Chonbuk National University) ;
- Park, Min-Kyung (Faculty of Bioresource Science, Chonbuk National University) ;
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Lee, Wang-Hyu
(Faculty of Bioresource Science, Chonbuk National University)
- 발행 : 2003.10.01
초록
Barely yellow mosaic(BaYMV) and barely mild mosaic (BaMMV) bymoviruses are both transmitted by the soil-inhabiting fungus Polymyxa gramnis, and are responsible for economic losses in barley crops in Asia and Europe. Because chemical control of the vector is ineffective, the losses can only be prevented by growing resistant barley cultivars. The objective of this study is to produce resistant barley plants by transformation with viral coat protein(cp) genes. Resistance tests of T1 plants transformed with the BaYMV CP gene showed that at least four independent lines had clear resistance to BaYMV but two other lines were highly susceptible with severe symptoms. The CP gene was detected in all resistant T1 plants by genomic PCR. Most of T2 progenies derived from the resistant T1 lines also showed resistance. In contrast, only one out of 21 independent T2 lines transformed with the BAMMV CP gene tested showed clear resistance to BaMMV, and others were very susceptible. Further analyses of resistance and CP gene expression are in progress.
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