한국식물병리학회:학술대회논문집 (Proceedings of the Korean Society of Plant Pathology Conference)
- 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
- /
- Pages.66.2-66
- /
- 2003
Rice genes specifically expressed in a rice mutant gained resistance to rice blast.(oral)
- C. U. Han (Biological Function Research Team, Korea Research Institute of Chemical Technology, Dept. Agricultural Biology, Chungbuk National University) ;
- Lee, C. H. (Biological Function Research Team, Korea Research Institute of Chemical Technology, Dept. Crop Science, Chung-Nam National University) ;
- K. S. Jang (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
- Park, Y. H. (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
- H. K. Lim (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
- Kim, J.C. (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
- Park, G. J. (Biological Function Research Team, Korea Research Institute of Chemical Technology) ;
- J.S. Cha (Dept. Agricultural Biology, Chungbuk National University) ;
- Park, J. E. (Dept. Crop Science, Chung-Nam National University)
- 발행 : 2003.10.01
초록
A gain-of-function mutant, SHM-11 obtained through gamma-ray mutagenesis, is resistant to rice blast caused by Magnaporthe grisea while wild type Sanghaehyanghyella is highly susceptible to the same disease. The resistance in the mutant was not race-specific when we tested with four races (KJ-201, KI-1113a, KI-313, KI-409) of M. grisea. To identify genes involved disease resistance in the gain-of-function mutant, genes specifically expressed in the mutant were selected by suppression subtractive hybridization using cDNAS of blast-inoculated mutant and wild type as a tester and a driver, respectively, Random 200 clones from the subtracted library were selected and analyzed by DNA sequencing. The sequenced genes represented three major groups related with disease resistance; genes encoding PR proteins, genes probably for phytoalexin biosynthesis, and genes involved in disease resistance signal transduction. A gene encoding a putative receptor-like protein kinase was identified as highly expressed only in the gain-of-function mutant after blast infection. The role of the putative receptor-like protein kinase gene during blast resistance will be further studied.
키워드