Functional pathogenomics of Burkhozderia glumae (oral)

  • Kim, Jinwoo (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Suhyun (School of Agricultural Biotechnology, Seoul National University) ;
  • Yongsung Kang (School of Agricultural Biotechnology, Seoul National University) ;
  • Jang, Ji-Youn (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Jung-Gun (School of Agricultural Biotechnology, Seoul National University) ;
  • Lim, Jae-Yoon (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Minkyun (School of Agricultural Biotechnology, Seoul National University) ;
  • Ingyu Hwang (School of Agricultural Biotechnology, Seoul National University)
  • 발행 : 2003.10.01

초록

The aim of this study was to characterize the interactions of rice and Burkholderia glumae, a causal agent of bacterial grain rot of rice, at molecular levels using whole genomic sequences and to identify genes important for pathogenicity and symptom development. To do these, we sequenced whole genome of the bacterium and constructed cosmid clone profiles. We generated pools of mutants using various transposons and determined mutation sites by sequencing rescued plasmids. We focused on studying toxoflavin biosynthetic genes, quorum sensing regulation, and Hrp type III protein secretion systems. We found that two possible operons consisting of five genes are involved in toxoflavin biosynthesis and their expression is regulated by quorum sensing and LysR-type regulator, ToxR. We have isolated the nn PAI of B. glumae and characterized by mutational analyses. The hrp cluster resembled most the putative Type III secretion systems of B. pseudomallei, which is the causative agent of melioidosis, a serious disease of man and animals. The Hrp PAI core region showed high similarity to that of Ralstonia solanacearum and Xanthomonas campestris, however some aspects were dissimilar.

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