Gene Expressions in Bovine Nuclear Transferred Embryos with Mouse Fetal Fibroblast Cell

  • Park, Sang-Hyun (Department of Animal Science, Chungbuk National University) ;
  • Park, Sang-Wook (Department of Animal Science, Chungbuk National University) ;
  • Yun, Ji-Yung (Department of Animal Science, Chungbuk National University) ;
  • Jun, Soon-Hong (Department of Animal Science, Chungbuk National University) ;
  • Kim, Nam-Hyung (Department of Animal Science, Chungbuk National University)
  • Published : 2002.06.01

Abstract

Interspecies nuclear transfer has been interested to determine ability of oocyte cytoplasm to support reprogramming of somatic cell nuclei of different species. In this study, we investigated developmental ability and mRNA expression patterns of developmentally important genes in bovine reconstructed embryos using a mouse fibroblast cell nucleus. While 20% nuclear transferred embryos with bovine fibroblast developed to morulae/blastocysts, a few(2-5%) nuclear transferred bovine embryos with mouse fibroblast developed to morula. (omitted)

Keywords