Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Analysis of Phytochemicals in Popular Medicinal Herbs by HPLC and GC-MS

HPLC와 GC/MS를 활용한 약용식물 유래 phytochemicals 분석

  • Cho, Hyun-Jung (Department of Applied Chemistry, Kumoh National Institute of Technology) ;
  • Yoo, Dong-Chan (Department of Applied Chemistry, Kumoh National Institute of Technology) ;
  • Cho, Hyun-Nam (Department of Applied Chemistry, Kumoh National Institute of Technology) ;
  • Fan, Lu-An (Department of Applied Chemistry, Kumoh National Institute of Technology) ;
  • Kim, Hee-Joon (Department of Applied Chemistry, Kumoh National Institute of Technology) ;
  • Khang, Kong-Won (Chephilos Corporation) ;
  • Jeong, Ho-Soon (Chephilos Corporation) ;
  • Yang, Seun-Ah (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Lee, In-Seon (The Center for Traditional Microorganism Resources, Keimyung University) ;
  • Jhee, Kwang-Hwan (Department of Applied Chemistry, Kumoh National Institute of Technology)
  • 조현정 (금오공과대학교 응용화학전공) ;
  • 유동찬 (금오공과대학교 응용화학전공) ;
  • 조현남 (금오공과대학교 응용화학전공) ;
  • 판루안 (금오공과대학교 응용화학전공) ;
  • 김희준 (금오공과대학교 응용화학전공) ;
  • 강공원 ((주)케피로스) ;
  • 정호순 ((주)케피로스) ;
  • 양선아 (계명대학교 전통미생물자원개발 및 산업화연구센터) ;
  • 이인선 (계명대학교 전통미생물자원개발 및 산업화연구센터) ;
  • 지광환 (금오공과대학교 응용화학전공)
  • Published : 2008.06.30
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Abstract

Oriental herbs are reported as having potent functions for preventing many types of diseases. They also appear to have positive effects and potential capabilities for skin care. Among the many oriental herbs that are available, we chose to analyze four medicinal herbs, Korean red ginseng, Artemisia capillaries Thunb, Schizonepeta tenuifolia Briq, and Foeniculum vulgare Mill, because all are popular and considered as favorite medicinal plants in Korea. Extracts of the herbs were obtained by various methods such as using distilled water, ethyl ether, methanol, ethanol, benzene, 1-butanol, and chloroform. Nine phytochemicals were detected in the extracts: maltol, adenosine, b-pinene, menthone, pulegone, limonene, anethole, estragole, and fenchone, which reportedly have multi-functionalities. All phytochemicals were analyzed quantitatively by various chromatographic techniques such as HPLC and gas chromatography-mass (GC-MS) spectrometry. This article also presents the optimum conditions for extracting these 9 targeted phytochemical compounds that were derived from 4 popular oriental herbs, which could be useful for the efficient preparation of each phytochemical.

본 연구는 홍삼, 인진쑥, 형개, 회향으로부터 기능성 성분을 분리 및 검출하는 것을 목적으로 하였다. 홍삼의 열수 추출물에 함유된 maltol과 adenosine을 HPLC를 이용하여 분리할 수 있었다. 그 중 maltol은 그 분획을 얻어 UV-visible 흡광도의 측정과 MS를 이용한 분자량 측정을 통하여 maltol 임을 재확인하였다. 그리고 인진쑥, 형개, 회향을 여러 가지 용매로 추출하여 그 추출물을 GC-MS로 분석한 결과, 인진쑥에는 limonene, menthone, anethole 등의 성분이 미량 검출되었고 회향에는 limonene 외의 성분은 거의 검출이 이루어지지 않았다. 본 실험 중 형개 추출물의 추출 효율이 가장 우수했는데 목적 성분 중 limonene, menthone, pulegone을 고농도로 얻을 수 있었다. 본 연구 결과로서 천연 약용 식물에서 기능 성분의 분리, 정제 시 각 성분에 대한 화학적 안전성을 확보할 수 있었으며, 한방 약초로부터의 기능성 화학성분의 정량분석을 HPLC, GC-MS 등의 분석방법으로 수행하였다. 본 실험의 결과는 향후 목적하는 phytochemicals을 함유한 복합체 기능성 연구에 사용하고자 한다. 약용식물 유래의 유용화합물과 안정성이 확보된 고분자 형성물질에 의해 만들어진 복합체는 새로운 기능을 나타낼 수 있으며, 식품, 화장품, 의약품 등에 다양한 이용가능성이 예상된다.

Keywords

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