• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.483-489
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• 2008

Nine newly isolated mushroom strains were tested to assess both their zinc tolerance and potential for zinc removal from an aqueous solution. Four strains of ectomycorrhizal fungi, namely Clavariadelphus truncatus (T 192), Rhizopogon roseolus (T 21), Lepista nuda (T 373), and Tricholoma equestre (T 174), along with five strains of white rot fungi, Lenzites betulina (S 2), Trametes hirsuta (T 587), Ganoderma spp. (T 99), Polyporus arcularius (T 438), and Ganoderma carnosum (M 88), were investigated using zinc-amended solid and liquid media. Their biosorption properties were also determined. The colony diameter and dry weight were used as tolerance indices for fungal growth. C. truncatus and T. equestre were not strongly inhibited at the highest concentrations of (225 mg/l) zinc in solid media. The most tolerant four strains with solid media, C. truncatus, G carnosum, T. hirsuta, and T. equestre, were then chosen for tolerance tests in liquid media. An ectomycorrhizal strain, C. truncatus, was also detected as the most tolerant strain in liquid media. However, the metal-tolerant strains demonstrated weak activity in the biosorption studies. In contrast, the highest biosorption activity was presented by a more sensitive strain, G. carnosum. In addition, seven different biosorbent types from G. carnosum (M 88) were compared for their Zn (II) biosorption in batch experiments.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.115-115
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• 2017

Zinc finger proteins constitute a large family which has been studied to have various functions in different organisms. Tandem CCCH zinc finger proteins (TZFs), members of the zinc finger protein family, are known to participate as post-transcriptional regulators of gene expression in eukaryotes. Here, we showed that the OsZn15, a gene for tandem CCCH zinc finger protein, is induced by abiotic stress and its overexpression in transgenic rice plants (PGD1:OsZn15) gains higher drought tolerance. Gene expression analysis of promoter:GFP plants revealed that OsZn15 is specifically expressed in anther and embryo, but not in vegetative organs. In-field evaluation, grain yield was higher in the PGD1:OsZn15 than nontransgenic plants under drought conditions. Interestingly, OsZn15 is shown to not only localize at nucleus but also co-localize with both processing bodies (PB) and stress granules (SG), two messenger ribo-nucleoprotein complexes which are known to activate by forming cytoplasmic foci under stress conditions. In sum, these results suggest that OsZn15 increases drought stress tolerance of rice probably by participating in RNA turnover in PB and SG.

• Bulletin of the Korean Chemical Society
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• 제30권10호
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• pp.2283-2286
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• 2009

A convenient route for the preparation of water soluble polythiophenes is described. Reactions involving highly active zinc metal show unique properties, viz. tolerance of the ester group and regioselectivity to the thiophene ring. Poly [3-(ethyl-n-alkanoate)thiophene-2,5-diyl]s, poly [3-(n-carboxyalkyl)thiophene-2,5-diyl]s, and poly [3-(potassium- n-alkanoate)thiophene-2,5-diyl]s were easily prepared by utilizing highly active zinc.

• Journal of Applied Biological Chemistry
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• 제60권2호
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• pp.113-117
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• 2017

생물학적정화는 미생물을 이용하여 중금속을 포함한 오염물질을 정화하는 방법이다. 효모는 다양한 중금속을 흡착하여 정화하는 능력이 우수하다고 잘 알려졌다. 따라서 본 연구는 여러 중금속 중 하나인 아연에 저항성인 큰 효모를 인위적인 돌연변이를 통해서 얻는 것에 초점을 두었다. 대조구인 효모를 1 mM 농도의 아연이 포함된 배지에서 키운 후 점차 아연의 농도를 높여 최종적으로 80 mM이 되는 지점까지 키웠으며 이 농도는 효모가 점진적 적응을 거쳤어도 성장이 불가능한 한계점 근처이며 이렇게 유도 분리된 효모를 ZnR이라 명명하였다. ZnR은 대조구 효모보다 아연에 대한 저항성이 현저히 증가되었으며 이 외에 카드뮴과 니켈에 대한 저항성도 증가되었지만 구리에 대한 저항성은 오히려 감소 되었다. ZnR이 보여준 아연에 대한 저항성 증가는 유전자 돌연변이에 의한 영구적인 획득 결과이다. 단 기간 내에 아연에 대한 저항성 큰 효모의 인위적인 유도 및 분리는 생물학적정화 연구에 유용하게 쓰여질 정보이다.

• Journal of Plant Biotechnology
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• 제40권1호
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• pp.49-54
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• 2013

C3HC4-type RING zinc finger proteins essential in the regulation of plant processes, including responses to abiotic stresses. We previously isolated and examined the C3HC4-type RING zinc finger protein (BrRZFP1) from Brassica rapa under abiotic stresses. To elucidate the role of the BrRZFP1 transcription factor in gene regulation, we transformed tobacco plants with the BrRZFP1 gene. Plants were regenerated from 82 independently transformed callus lines of tobacco and analysed for transgene expression. Transgene integration and expression was confirmed by Southern and RT-PCR analyses, respectively. T2 plants displayed more tolerance to the bacterial pathogens Pectobacterium carotovorum and Ralstonia solanacearum, and the tolerance levels were correlated with BrRZFP1 expression levels. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and its overexpression in plants could increase biotic stress resistance.

• The Korean Journal of Ecology
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• 제18권1호
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• pp.147-156
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• 1995

Restoration of ecosystems degraded by heavy metal pollution can be accomplished by soil amendment and selection and utilization of plants tolerant to heavy metals. Two former zinc mine sites, Sambo Mine in Hwasung, Kyonggi-do and the Second Yonhwa Mine in Samchuk, Kangwon-do, were selected for collection of plant samples and for determination of heavy metal tolerant species. Dominant species on mine waste deposits in Hwasung site were Panicum bisulcatum and Echinoch/oa crus-galli, while those in Samchuk site were Aster yomena, Setaria viridis, Artemisia lavandulaefolia and Oenothera odorata. Mean contents of zinc, lead and cadmium in Hwasung soil were 103, 117 and 1 ppm, respectively, while those in Samchuk soil were 23, 6 and 4 ppm, respectively, Zinc contents were higher in Echinochloa crus-galli from Hwasung and in Artemisia lavandulaefolia from Samchuk, while lead contents were higher in Panicum bisulcatum and Echinochloa crus-galli from Hwasung and Lactuca sonchiJolia and Pinus densiJolia from Samchuk. Plant species with higher cadmium contents were Panicum bisulcatum and Lactuca sonchiJolia. Comparison of metal contents between roots and shoots showed that Echinochloa crus-galli was a zinc accumulator, while Panicum bisulcatum, Persicaria hydroPiPer, Pinus densiJlora and Lactuca sonchiJolia were zinc excluders. In addition, Panicum bisulcatum and Persicaria hydroPiPer were proved to be lead excluders. When both heavy metal contents in plant tissues and biomass of individual plants are considered, it can be concluded that Echinochloa crus-galli and Panicum bisulcatum from Hwasung and Artemisia lavandulaefolia and Aster yomena are heavy metal absorbing plants. The effect of heavy metals on seed germination showed that Artemisia princeps var. orientalis had higher germination rates, but no significant difference in concomitant decrease of germination rates among the species investigated were found by increasing heavy metal contents.

• Nutrition Research and Practice
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• 제6권3호
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• pp.221-225
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• 2012

Zinc deficiency is known to be associated with insulin resistance in obese individuals. This study was performed to evaluate the effect of zinc supplementation on insulin resistance and metabolic risk factors in obese Korean women. Forty obese women (body mass index (BMI) ${\geq}25kg/m^2$) aged 19-28 years were recruited for this study. Twenty women of the study group took 30 mg/day of supplemental zinc as zinc gluconate for 8 weeks and 20 women of control group took placebo. Usual dietary zinc intake was estimated from 3-day diet records. Insulin resistances were measured using Homeostasis model assessment (HOMA) indices, and insulin sensitivities Matsuda indices, which were calculated using oral glucose tolerance test data. Metabolic risk factors, such as waist circumference, blood pressure, fasting glucose, triglyceride, high density lipoprotein (HDL) cholesterol, and adipocyte hormones such as leptin, and adiponectin were also measured. At the beginning of study, dietary zinc averaged 7.31 mg/day and serum zinc averaged $12.98{\mu}mol/L$ in the study group. Zinc supplementation increased serum zinc by 15% and urinary zinc by 56% (P < 0.05). HOMA values tended to decrease and insulin sensitivity increased slightly in the study group, but not significantly so. BMI, waist circumference, blood pressure, blood glucose, triglyceride, HDL cholesterol, and adipocyte hormones did not change in either the study or control group. These results suggest that zinc status may not affect insulin resistance and metabolic risk factors in obese Korean women. Further research is required on a larger cohort with a longer follow-up to determine the effects of zinc status on insulin resistance and metabolic variables.

• Journal of Nutrition and Health
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• 제54권6호
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• pp.594-602
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• 2021

본 연구는 고지방식이로 유도한 비만 동물모델에서 아연의 식이를 통한 보충 급여가 혈당 조절과 골격 근육의 ZIP7의 작용에 미치는 영향에 대해 살펴보았다. 고지방식이를 공급한 HF군은 정상 대조군에 비하여 단위 체중당 골격 근육 무게가 유의하게 감소하였으며, 혈당은 유의적으로 증가하였다. 고지방식이와 함께 아연을 보충 공급한 HF+Zn군은 아연을 보충하지 않은 HF군과 비교하여, 공복 혈당과 경구 포도당 부하 후 혈당 증가 면적이 유의하게 감소하였다. 또한, HF+Zn군은 HF군에 비해 골격 근육의 ZIP7 단백질 수준이 유의하게 증가하였으며, AKT 활성과 GLUT4 단백질 수준도 유의하게 증가하는 것으로 나타났다. 이상의 결과를 종합해 볼 때, 아연 보충은 비만으로 인한 고혈당 증세를 완화하는 효과를 나타내며, 이는 골격 근육에서의 ZIP7 아연 수송체에 의한 당 대사 조절과 관련이 있을 것으로 생각된다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제15권2호
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• pp.18-23
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• 2010

Usability of soil quality monitoring network for ascertaining soil quality changes was evaluated by analysing soil quality monitoring results. Tolerance limits of soil quality monitoring results from 1997 to 2007 were calculated and compared with Korean soil quality standards. This study determined that soil quality was changed if the upper 95% tolerance limit value was greater than the soil quality standard. Fluoride most frequently exceeded the soil quality standard and nickel, zinc, arsenic, copper, lead and cadmium were followed. Analysis on land use showed that tolerance limits of industrial land use most frequently exceeded the soil quality standards and residential, road and various land uses then frequently exceeded. Tolerance limits of land uses expecting high contaminant loads frequently exceeded the soil quality standards. This fact imply that the soil quality monitoring network generates reasonable data to represent change in Korean soil quality. This study also suggested that representative sampling from well identified points should be done to improve data reliability and accurately ascertain soil quality changes.

• Journal of Ecology and Environment
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• 제30권3호
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• pp.257-264
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• 2007

Over-expression of a copper/zinc superoxide dismutase (Cu/ZnSOD) resulted in substantially increased tolerance to cadmium exposure in Arabidopsis thaliana. Lower lipid peroxidation and $H_2O_2$ accumulation and the higher activities of $H_2O_2$ scavenging enzymes, including catalase (CAT) and ascorbate peroxidase (APX) in transformants (CuZnSOD-tr) compared to untransformed controls (wt) indicated that oxidative stress was the key factor in cadmium tolerance. Although progressive reductions in the dark-adapted photochemical efficiency (Fv/Fm) and quantum efficiency yield were observed with increasing cadmium levels, the chlorophyll fluorescence parameters were less marked in CuZnSOD-tr than in wi. These observations indicate that oxidative stress in the photosynthetic apparatus is a principal cause of Cd-induced phytotoxicity, and that Cu/ZnSOD plays a critical role in protection against Cd-induced oxidative stress.