• 동의생리병리학회지
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• 제16권4호
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• pp.774-781
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• 2002

This study was carried out to test the recuperative effect of 2 types of sample drugs for 3 degree burn. The burn injury was made by iron plate heated in the boiling water. The Sprague Dawley rats were shaven with a razor preliminarily and burned by direct contact method for 10 seconds. The experimental groups were classified with 5 each-normal, control, MEBO ointment, sample A, sample B. The effect of the sample drugs were decided by histological results after 3 week application. The results were as following. The 3 treatment groups recovered the burn injury faster than control group. The recuperative effect precedes about 4-5 days at the time of 15th day and 1 week at the time of 3rd week. The therapeutical procedure of 3 treatment groups was similar with naked eye and with microscopic histology in the 1 st, 2nd and 3rd specimens. So there weren't significant differences in curative effect in 3 treatment groups of this experiment. But a regeneration of hair follicle was noted in Sample B uniquely. These results suggested that 3 burn remedies have similar effect of therpy, but sample B containing yolk sac oil has slightly better effect in part of hair regeneration.

• 대한수의학회지
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• 제34권2호
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• pp.375-379
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• 1994

A teratogenicity test of 'folpet' was carried out in the developing chick embryos to investigate and validate the safety of rural environmental hazardous materials. Folpet was administered to chick embryos' yolk sac at a rate of 0.1mg and 0.01mg per SPF eggs at 96 hours of incubation. The morphological changes were examined. Fertility ratio of SPF eggs used was 94.9%. Hatching rate of untreated control group was 74.4% and the group dosed with 100ul of corn oil into the yolk sac was 70.0%. The $LD_{50}$ of folpet was 0.663mg/100ul/egg. After hatching, mean body weight, body length, claw length and beak length of high and low dose administered groups were not significantly different from untreated and vehicle control group. There was no abnormal appearence in all the groups. Therefore it seems that, within the doses applied, folpet dose not induce potential teratogenicity in the developing chick embryos.

• 한국발생생물학회지:발생과생식
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• 제1권1호
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• pp.91-97
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• 1997

In order to study the reproductive physiology of Sand ell(Hypoptichus dybowskii) catched at kangwon-do, Wooamjin Bay, spawning and egg development were investigated in April and May, 1996. The observed spawning period was from the middle of April to the end of May with the water temperature above $8^{\circ}$C. Body size and colouration were distinguished between male and female. The type of testicular structure is lobular, and the ovary is a pair of sac-sahaped covering with a fibromuscula capsule and comprising a mature egg of average 86. The unfertilzed egg are demersal and adhesive, and their diameter were 1.65-1.95 mm. the colour of yolk is light yellow and contained a lot of tiny oil globules. The average spawned egg was 62 in sargasso weeds. Hatching was observed in 12 days after fertilization with the water temperature of 14 ${\pm}1^{\circ}$C. The average size of larva after hatching was 6.85${\pm}$0.23mm in total length, and larva were containing yolk including globule.

• 한국수산과학회지
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• 제41권6호
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• pp.471-477
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• 2008

Development of egg, larvae and juveniles for the Pacific mackerel, Scomber japonicus are described following natural fertilization in the indoor tank of $25^{\circ}C$ water temperature. Following a routine hormone treatment technique for the brood stock, male and female mackerels were artificially matured by intramuscular injections of LHRHa at a dosage of $400{\mu}g/kg$ body weight (BW)+Domperidone at a dosage of $4{\mu}g/kg$ body weight (BW) to induce maturation in a separate aquarium and induced natural spawning. Fertilized eggs were ca. 1.0 mm in diameter; spherical in shape with a single oil globule; pelagic and non-adhesive. Hatching occurs 41 hours after fertilization at $23-24^{\circ}C$. The newly hatched larvae was 3.03 mm in average total length (ATL), the mouth and anus were not open, oil globule located in posterior end of yolk sac, and preanal length was 42.8% of TL. The larvae measuring 2.89 mm ATL, almost absorbed yolk sac and oil globule material in 2 days after hatching, in which the mouth and anus were open. Melanophores, branch or star in shape were observed on the top of head, peritoneal region and along the ventral contour. In 13 days after hatching, the larvae was 6.88 mm ATL, its posterior end of notochord began to flex upward, finfold of caudal fin appeared, jaw teeth were already formed. In 19 days after hatching, the larvae was 7.71 mm ATL completed only caudal fin rays (9+8), and preanal length was 49.4% of TL. In 37 days after hatching, the larvae was 27.4 mm ATL already completed all the fins, and preanal length was 59.9% of TL.

• The Korean Journal of Ecology
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• 제19권5호
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• pp.477-486
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• 1996

The freshwater goby, Micropercops swinhonis ($G\"{u}nther$) was studied on the spawning behavior and egg develeopment at the two areas of Chollabukdo, Korea from March to May 1995. Egg mass attached to a layer under stones and the inner wall of waterplant, Cyperus. Spawning behavior was divided in to 1) premating stage: selecting spawning sites and formation of territory by male, 2) mating stage: enticement with zigzag-like dance and intermittant shaking of head by male and fertilization, 3) postmating stage: fanning, guarding and cleaning the nest by male. The eggs were transparent and spherical in shape, measuring $0.9\times1.4mm$ with yellowish yolk sac and many oil globules. Hatching began in about ten days after fertilization at water temperature $16~20^{\circ}C$. The newly hatched larvae were 3.8~4.0 mm in total length with 30 myomeres and their mouths and anuses were opened. Melanophores appeared at the air-bladder and the ventral side of caudal region.gion.

• Applied Microscopy
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• 제28권3호
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• pp.273-282
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• 1998

Ultrastructure of fertilized egg envelope in the tomato clown anemonefish (Amphiprion freatus) was examined by mean of light, scanning and transmission electron microscopies. The fertilized egg of tomato clown anemonefish was of non-transparent, ellipsoidal, adhesive and non-floted type, and there were numerous oil droplets in the yolk sac and adhesive filaments in the area of the animal pole. The outer surface of egg envelope was smooth. The fertilized egg envelope consisted of two distinct layers; a non-adhesive outer electron-dense layer and an inner layer, consisting of five horizontal low electron-dense lamellae alternating with the middle electron dense interlamellae.

• 한국수산과학회지
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• 제3권4호
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• pp.233-250
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• 1970

감성들의 난과 부화자어의 특성 및 활력에 대카여 관찰한 결과를 요약하면 다음과 같다. 1) 감성돔의 완숙난은 한개의 유구를 가진 투명하고 구형인 분리부성난이며, 난경은 $0.81\~0.96mm$의 범위이며 유구경은 $0.20\~0.23mm$이다. 2) 감성돔의 수정난은 애정후 3시간 50분만에 부화하고, 부화자어의 크기는 전장이 1.57mm이며, 특히 부화직후의 난황상에는 한점의 색소세포도 존재하지 않는다. 3) 감성돔의 친어별 완숙난의 크기는 대소 2형으로 구분되는데, 소형난의 경우 난경은 최소 0.811mm, 최대 0.873mm이고, 대형난에 있어서는 최소 0.874mm 최대 0.963mm이다. 4) 감성돔의 부화율은 대형난이 $78.63\~95.19\%$, 소형난이 $68.84\~74.80\%$이며, 대형난의 경우가 성적이 좋았다. 따라서 전체 수정난의 평균 부화율은 $79.80\%$였다. 5) 감성돔의 부화에 필요한 부화적온은 $20\~26^{\circ}C$의 범위이며, 고수온에 있어서의 부화가능한계온도는 $26.71\~28.56^{\circ}C$ 전후이다. 6) 감성돔의 부화자어의 전장은 난경과는 병행관계를 이루지 않고, 대형자어는 $1.976\~2.225mm$의 범위이며, 소형자어는 $1.526\~1.90mm$의 범위에 있다. 7) 감성돔의 부화자어의 난황경은 $0.878\~0.979mm$의 범위이다. 8) 감성돔의 부화자어의 초기생잔율은 무투이상태에서 5일간을 기준으로 볼 때 최소 $13.71\%$, 최대 $79\%$로 평균초기생잔율 $48.3\%$였다. 9) 이상과 같은 감성동의 부화자어의 생물학적 특성 및 활력으로 볼 때 인공종자의 대량생산의 가능성은 충분히 있다고 사료되지만 기초이료를 위주로 한 사육실험의 결과등이 보충되어야 할 것으로 생각된다.

• 한국식품위생안전성학회지
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• 제14권1호
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• pp.60-66
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• 1999

Diethylnitrosamine (DEN) is known as a potential hepatic carcinogen by single administration. This study was designed to measure the effects of DEN-induced cell damage on the triglyceride and cholesterol concentration in the liver, excluding dietary effects. Fertilized chicken eggs, 10 days before hatching, were randomly divided into three groups (n=20) and each egg was injected 10 ${mu}ell$ of corn oil (vehicle control), 5 $\mu\textrm{g}$ of DEN/10 ${mu}ell$ of DEN/10 ${mu}ell$ into yolk via air sac. After 48 hr and 96 hr incubation, the damage of the chick-embryo liver cell was investigated by electron microscopy and by measuring the concentration of lipid components (total cholesterol, free cholesterol, phospholipid and triglyceride). For eggs administered 10 $\mu\textrm{g}$ of DEN and incuvated 96 hr, in hepatocyte, the nucleus membrane was roughed, the size of nucleolus was apparently increased and euchromatin was accumulated. Mitochondria were condensed and cristae, located mitochondiral inner membrane, were obscured. Additionally, the leaves of triglyceride and cholesterol classes were significantly increased depend on the amount treated with 10 $\mu\textrm{g}$ DEN at 96 hr, but phospholipids component of cell membrane, were decreased with significance. As a conclusion, carcinogen induced hepatic lesion was correlated with the changes in lipid component of liver.

• 한국수산과학회지
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• 제29권4호
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• pp.497-502
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• 1996

1990년 3월, 경상북도 영덕군 강구면에 위치한 오십천과 경상남도 밀양시 밀양강에서 채집하여 인공수정한 난을 실험실로 운반하여 난발생과정과 부화자어의 성장에 따른 형태변화를 관찰한 결과는 다음과 같다. 1. 빙어의 수정난은 구형의 침성부착난으로 무색 투명하며, 난경은 $0.85\~1.05mm$ (평균 0.97mm, n=50)이다. 2. 부화에 소요된 시간은 평균 $16.5^{\circ}C$에서 약 170시간만에 부화한다. 3. 부화직후의 자어는 전장 $3.85\~4.25mm$ (평균 4.05 mm, n=10)로, 아직 입과 항문이 열려 있지 않고, 눈에 색소포가 착색되어 있으며, 근절수는 $52\~54$개이다. 4. 부화 $4\~5$일째의 자어는 전장이 $5.20\~5.65mm$ (평균 5.37mm, n=10)로 난황은 거의 흡수되고, 눈의 색소는 더욱 짙어진다. 5. 부화 20일째의 후기자어는 전장이 $8.20\~8.55\;mm$(평균 8.38mm, n=10)로 척색말단이 $45^{\circ}$ 각도로 위로 굽어진다. 6. 부화 25일째의 후기자어는 전장이 $9.15\~9.75mm$ (평균 9.63mm, n=10)로 등지느러미와 뒷지느러미에 각각 융기하기 시작하여 원기가 나타난다.

• 한국수산과학회지
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• 제19권4호
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• pp.368-374
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• 1986

1986년 1월 16일에 강원도 명주군 강동면 정동진리 앞 1 kim 연해에서 삼중자망으로 잡은 미거지 친어(전장: 60cm, 전장: 70m)를 채포 즉시 선상에서 건도법으로 인공수정시켜 실험실로 운반하여 난발생과정과 부화자어에 대해 관찰한 결과를 요약하면 다음과 같다. 성숙란은 많은 유구를 지니며, 난황이 담황색 또는 담자색인 구형의 침성점착난으로 난경은 $1.55{\sim}1.65mm$(평균, 1.59mm, n=28)이다. 실내사육수온 $7.09{\sim}10.99^{\circ}C$(평균, $8.79^{\circ}C$), 염분농도 $30.62{\sim}33.98\%0$(평균, $32.32\%0$)에서 수정후 747시간만에 부화하였다. 부화직후의 자어는 전장 $4.40{\sim}4.85mm$로서 근절수 $12{\sim}13+40=52{\sim}53$이며, 아래턱, 난황등쪽, 배쪽 그리고 가슴지느러미위에 수지상의 흑색소포가 발달하며, 꼬리부분의 배쪽 막지느러미와의 경계상에 일열로 병행하여 흑색소포가 나타나 있다. 부화후 3일째의 자어는 전장 $5.15{\sim}5.25mm$이고, 배쪽 막지느러미위에 갈색소포가 줄지어 나타나며, 이포뒤에 몇개의 흑색소포가 나타난다. 부화후 7일째의 자어는 전장 $5.75{\sim}5.85mm$로서 난황이 상당히 흡수되고 두부가 커지며, 소화관이 발달한다.