• Proceedings of the Botanical Society of Korea Conference
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• 1995.06a
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• pp.79-90
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• 1995

Production of a cardiac glycoside, digoxin, by 12$\beta$-hydroxylation from digitoxin was studied in plant cell suspension cultures of Digitalis lanata. In order to increase the conversion yield, various culture conditions including immobilization were investigated and optimized. Since digoxin was released in the medium temporarily and converted further into a glucosylated product, deacetyllanatoside C, in situ adsorption of digoxin was employed to recover the product continuously. Amberlite resin XAD-8 showed the best adsorption characteristics for digoxin among the examined resins, and an integrated process was developed to increase the productivity. In addition, it was found that the utilization of $\beta$-cyclodextrin to entrap digoxin during the culture enhanced the biotransformation yield significantly.

• Journal of Institute of Convergence Technology
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• v.3 no.1
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• pp.41-44
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• 2013

Process and manufacturing data are numerously accumulated to the enterprise database in industries but little of those data are utilized. Data mining can support a decision to manager in process from the data. However, it is not easy to field managers because a proper adoption of various schemes is very difficult. In this paper, six scenarios are conducted using data mining schemes for the various situations of field claims such as yield problem, trend analysis and prediction of yield according to changes of operating conditions, etc. Scenarios, like templates, of various analysis situations are helpful to users.

• Transactions of the Korean Society of Automotive Engineers
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• v.6 no.1
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• pp.99-107
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• 1998

Stress distribution analysis was implemented by using finite element method for the lower arm connecting Independent front suspension. Results were obtained for the 8 load conditions and for the 3 types of section (I, H and H+I). On the basis of it, the shape and dimensions of lower arm were optimized. Finally it was pointed out that the H type has an most satisfied strength, among 3 section types and highest safety factor and yield strength in each case of load condition.

• Journal of Biosystems Engineering
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• v.13 no.3
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• pp.59-70
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• 1988

A computer simulation model for rice circulating concurrent-flow dryer was developed and verified by conduction a series of pilot-scale experiment. The effects of design parameter and operating conditions on dryer performance were analyzed by using simulation. The results indicated that the developed model was found suitable for analyzing operating characteristics. The other results from simulation also showed that; 1) an increse in the initial moisture content resulted in an increase in the drying rate and a reduction in the grain temperature and total energy requirements. 2) an increase in the drying air temperature resulted in an increase in the drying rate and grain temperature. 3) an increase in air flow rate resulted in an radical increase in drying rate, fan power requirements and total energy requirements but an radical decrease in final head rice yield. 4) an increase in the bed depth resulted in an increase in fan power requirements and a lowering of the final head rice yield.

• Journal of the Korean Applied Science and Technology
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• v.23 no.1
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• pp.12-18
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• 2006

The transesterification of vegetable oils into Biodiesel at $60^{\circ}C$ was performed on the rotary viscometer. The overall yield(%) of fatty acid methyl ester from canola oil at optimum conditions was 95%. The viscosities of fatty acid methyl esters were predicted by Orrick and Erbarr's model. The overall yield increased as the viscosities of fatty acid methyl esters decreased. The limiting molar ratio of methanol to oil appeared to be 1:5. The content of sodium hydroxide as the optimum catalyst appeared to be 0.5wt%.

• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.399-403
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• 1999

For the production of trehalose, microorganisms capable of producing trehalose extracellularly were screened from the stock cultures in our laboratory. among them, Micrococcus luteus IFO 12708 showed the highest productivity of trehalose. For the increase of productivity, the mutant strai Hs-208 having higher trehalose production was selected with NTG(N-methyl-N'-nitrosoguanidine) mutagenesis, which led to the decrease of the specific activity of trehalose phosphorylase(3.2-fold) as compared to the wild strain. The optimum condition for the trehalose production was established as follows: 20g/l of glucose and 6g/l of tryptone were used as a sole carbon source and nitrogen source, respectively, and cultivations were carried out at 3$0^{\circ}C$ and pH 6.0. After 20hrs cultivation, addition of 20unit/ml penicillin G led to the higher conversion yield of trehalose. Under the optimum condition, 6.547g/l trehalose was produced with conversion yield of 32.7%.

• Proceedings of the Korean Environmental Health Society Conference
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• 2003.06a
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• pp.106-110
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• 2003

The study was carried out immobilized Aspergillus niger used of milk-wastewater. The purpose of investigation is to optimize the ermentational conditions of milk-wastewater The optimal pH, temperature and dilution rate were 3.0, 30$^{\circ}C$ and 0.025 h$\^$-1/. The maximum amount and yield of citric acid produced by immobilizes Aspergillus niger ATCC 9142 were 4.5g/1 and 70.3%. Compared to shake-flask culture, yield was increased about 20%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.1
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• pp.73-77
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• 1993

This study was intended to investigate the effects of solvents, particle size of a sample, sample to solvent ratio, temperature and time on the extraction of garlic oleoresin. Among eleven solvents used for oleoresin extraction from garlic, the optimal solvent was methyl alcohol. The most appropriate particle size of garlic, extracting temperature and mixing ratio of garlic to methyl alcohol were 20mesh, $25^{\circ}C$ and 1 to 3(w/w), respectively. On the basis of yield in oleoresin extraction, optimum extracting time was about 4 hours. The yield of oleoresin under the above-mentioned conditions was 21.3%. "L" and "b" as color appearance were decreased, whereas "a"was increased slightly during 60 days storage at 5$^{\circ}C$, $25^{\circ}C$ and 4$0^{\circ}C$. Changes in the pyruvate content showed close relation to pH value. During storage pyruvate content of garlic oleoresin was decreased as increasing storage temperature.

• BMB Reports
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• v.35 no.4
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• pp.428-431
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• 2002

The often-encountered problem of disrupting bacteria for the purpose of extracting soluble protein has generated various methods. Many require specialized equipment. Very often, especially during preliminary studies, investigators need a simple, fast, and inexpensive method for cell disruption that preserves biological activity. This paper compares some simple and inexpensive methods for cell disruption, such as bead-vortexing, freesing-thawing, French pressing, and sonication. It also provides some tips to increase protein yield and preserve biological activity. If performed under optimal conditions, bead-vortexing gives protein yields that are comparable to French pressing and sonication. It also preserves the activities of labile enzymes and releases periplasmic enzymes. Vortexing with glass beads appears to be the simplest method for cell disruption.

• Journal of the Korean Society for Precision Engineering
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• v.9 no.3
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• pp.61-66
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• 1992

This paper addresses on the determination of damping coefficients of an infinitely long squeeze film damper operating with an electro-rheological (ER) fluid. The ER fluid behaves as Bingham fluid with an electric field dependent yield shear stress. AS phenomenological model of the fluid is adopted for the relationship between the yield shear and the intensity of the electric field imposed on the fluid domain. The model is then incorporated with the governing equation and associated boundary conditions of the squeeze film damper executing a circula centered orbit for the expression of dimension- less damping coefficients. Numerical simulation is performed to evaluate the performance improvement of the proposed squeeze film damper.