• Title/Summary/Keyword: yield conditions

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Optimum Extraction Conditions and Anticancer Effect of Functional Polysaccharide from Mycelia of Grifola frondosa (잎새버섯(Grifola frondosa) 균사체의 기능성 다당류 최적 추출방법 및 항암효과)

  • Park, Chan-Ho;Lee, Gyeong-Min;Nam, Eun-Jeong;Yu, Yeon-Hee;Kim, Yong-Hyun;Kwon, Hyun-Jung;Yoon, Ok-Hyun;Han, Man-Deuk
    • The Korean Journal of Food And Nutrition
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    • v.25 no.1
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    • pp.181-187
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    • 2012
  • Grifola frondosa has been used as an herbal medicine for the treatment of cancer, diabetes mellitus and high blood pressure. In this study, functional polysaccharide was obtained from Grifola frondosa using four different extraction methods: hot water(HwFP), homogenize(HgFP), acid(AcFP), and alkali(AlFP) extraction methods. The effects of these extracts on KB and HepG2 cell lines were then examined for any anti-cancer activity. Alkaline extraction produced a yield of 0.175% and the total sugar content of the extract was 54.97%. We were able to confirm that the polysaccharide extracts from the mushroom produce an anti-cancer effect. The cytotoxicity of AlFP and AcFP against HepG2 cells were 22.86% and 28.88%, respectively, and the cytotoxicity of AlFP against the KB cell lines was 47.76% at a concentration of 1,000 ${\mu}g/m{\ell}$. Therefore, these results suggest that the optimum method for extracting functional polysaccharides from G. frondosa is the alkali extraction method.

Preparation and Characterization of a Polar Milk Lipid-enriched Component from Whey Powder

  • Lee, Kwanhyoung;Kim, Ara;Hong, Ki-Bae;Suh, Hyung Joo;Jo, Kyungae
    • Food Science of Animal Resources
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    • v.40 no.2
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    • pp.209-220
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    • 2020
  • Milk fat globule membrane (MFGM) is a lipid carrier in mammals including humans that consists mainly of polar lipids, like phospholipids and glycolipids. In this study, a process to enrich polar lipids in commercial butter and whey powder, including polar lipids of MFGM, was developed. WPC (whey protein concentrate) 60 was selected as the most suitable raw material based on the yield, phospholipid, protein, and lactose content of the polar lipid fraction obtained by ethanol extraction of two WPC (WPC60 and WPC70) and two buttermilk (A and B). After fractionation under optimum conditions, the polar-lipid enriched fraction from WPC60 contained 38.56% phospholipids. The content of glycolipids, cerebroside, lactosylceramide, ganglioside GM3, ganglioside GD3, was 0.97%, 0.55%, 0.09%, and 0.14%, respectively. Rancimat results showed that the oxidation stability of fish oil increased with an increase in the polar-lipid fraction by more than 30 times. In addition, the secretion of IL-6 and TNF-α decreased in a concentration-dependent manner after treatment of RAW 264.7 cells with 0.1 to 100 ppm of the polar lipid fraction. In this study, polar lipid concentrates with antioxidant and anti-inflammatory activity, were prepared from milk processing by-products. The MFGM polar lipid concentrates made from by-products are not only additives for infants, but are also likely to be used as antioxidants in cooking oils and as active ingredients for functional foods.

Impact of Air Convection on H3PO4-Activated Biomass for Sequestration of Cu (II) and Cd (II) Ions

  • Girgis, Badie S.;Elkady, Ahmed A.;Attia, Amina A.;Fathy, Nady A.;Abdel Wahhab, M. A.
    • Carbon letters
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    • v.10 no.2
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    • pp.114-122
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    • 2009
  • Crushed, depitted peach stones were impregnated activated with 50% $H_3PO_4$ followed by pyrolysis at $500^{\circ}C$. Two activated carbons were produced, one under its own evolved gases during pyrolysis, and the second conducted with air flow throughout the carbonization stage. Physicochemical properties were investigated by several procedures; carbon yield, ash content, elemental chemical analysis, TG/DTG and FTIR spectra. Porosity characteristics were determined by the conventional $N_2$ adsorption at 77 K, and data analyzed to get the major texture parameters of surface area and pore volume. Highly developed activated carbons were obtained, essentially microporous, with slight effect of air on the porous structure. Oxygen was observed to be markedly incorporated in the carbon matrix during the air treatment process. Cation exchange capacity towards Cu (II) and Cd (II) was tested in batch single ion experimental mode, which proved to be slow and a function of carbon dose, time and initial ion concentration. Copper was up taken more favorably than cadmium, under same conditions, and adsorption of both cations was remarkably enhanced as a consequence of the air treatment procedure. Sequestration of the metal ions was explained on basis of the combined effect of the oxygen functional groups and the phosphorous-containing compounds; both contributing to the total surface acidity character.

Essential Oil Yields and Chemical Compositions of Chamaecyparis obtuse Obtained from Various Populations and Environmental Factors

  • Kang, Young Min;Min, Ji Yun;Choi, Myung Suk
    • Journal of Forest and Environmental Science
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    • v.30 no.3
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    • pp.285-292
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    • 2014
  • Essential oil yields and chemical compositions from 5 populations of Chamaecyparis obtusa with several environmental factors were investigated through essential oil extracted distillation apparatus and metabolite profiling by GC-MS analysis. Among the populations, content of essential oil at Gokseong was significantly higher than other populations. To compare the several environmental factors affecting on chemical composition and essential oil yields from C. obtuse at Gokseong, the environmental factors (soil condition, temperature, humidity, and moisture content) were measured during 1 year. The essential oils at Goksung based on humidity on March, July, and November was significantly different from other months. The essential oils at Goksung based on temperature on July and August was significantly different from other months. The essential oils at Goksung based on the moisture content on September were significantly different from other months. The percentage of T-N, OM, and yield of oil at Gokseong were significantly different on from other populations. The main constituents of C. obtusa at all populations were ${\alpha}$-pinene, ${\beta}$-pinene, ${\alpha}$-terpinene, ${\gamma}$-terpinene, terpinene-4-ol, isobonyl acetate, terpinyl acetate, and cedar acetate. Specially, Essential oil compositions (%) of ${\alpha}$-terpinene and cedar acetate were higher at Gokseong than at other populations. The chemical compositions of essential oils were variable depend on populations and environmental conditions. Therefore, this study might be used as fundamental research on study for selection of high productive terpenoids and for understanding about biosynthesis of essential oils in C. obtusa.

Revision of Modified Cam Clay Failure Surface Based on the Critical State Theory (한계 상태 기반 수정 Modified Cam Clay 파괴면)

  • Woo, Sang Inn
    • Journal of the Korean Geotechnical Society
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    • v.36 no.4
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    • pp.5-15
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    • 2020
  • This paper proposes a revised Modified Cam Clay type failure surface based on the critical state theory. In the plane of the mean effective and von Mises stresses, the original Modified Cam Clay model has an elliptic failure surface which leads the critical-state mean effective stress to be always half of the pre-consolidation mean effective stress without hardening and evolution rules. This feature does not agree with the real mechanical response of clay. In this study, the preconsolidation mean effective stress only reflects the consolidation history of the clay whereas the critical state mean effective stress only relies on the currenct void ratio of clay. Therefore, the proposed failure surface has a distorted elliptic shape without any fixed ratio between the preconsolidation and critical state mean effective stresses. Numerical simulations for various clays using failure surfaces as yield surface provide mechanical responses similar to the experimental data.

Hydraulic Design of Natural Gas Transmission Pipeline in the Artic Area (극한지 장거리 천연가스 배관의 유동 설계)

  • Kim, Young-Pyo;Kim, Ho-Yeon;Kim, Woo-Sik
    • Journal of the Korean Institute of Gas
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    • v.20 no.2
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    • pp.58-65
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    • 2016
  • Hydraulic analysis of the natural gas transmission pipeline is to determine whether adequate flow can be sustained throughout the design life of pipeline under all expected flow conditions. Many factors have to be considered in the hydraulic design of long-distance pipelines, including the nature, volume, temperature and pressure of fluid to be transported, the length and elevation of pipeline and the environment of terrain traversed. This study reviewed the available gas operation data provided by pipeline construction project in the arctic area and discussed the gas properties such as viscosity and compressibility factor that influence gas flow through a pipeline. Pipeline inside diameter was calculated using several flow equations and pipeline wall thickness was calculated from Barlow's equation applying a safety factor and including the yield strength of the pipe material. The AGA flow equation was used to calculate the pressure drop due to friction, gas temperature and pipeline elevation along the pipeline. The hydraulic design in this study was compared with the report of Alaska Pipeline Project.

Deformation analysis of high CFRD considering the scaling effects

  • Sukkarak, Raksiri;Pramthawee, Pornthap;Jongpradist, Pornkasem;Kongkitkul, Warat;Jamsawang, Pitthaya
    • Geomechanics and Engineering
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    • v.14 no.3
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    • pp.211-224
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    • 2018
  • In this paper, a predictive method accounting for the scaling effects of rockfill materials in the numerical deformation analysis of rockfill dams is developed. It aims to take into consideration the differences of engineering properties of rockfill materials between in situ and laboratory conditions in the deformation analysis. The developed method is based on the modification of model parameters used in the chosen material model, which is, in this study, an elasto-plastic model with double yield surfaces, i.e., the modified Hardening Soil model. Datasets of experimental tests are collected from previous studies, and a new dataset of the Nam Ngum 2 dam project for investigating the scaling effects of rockfill materials, including particle size, particle gradation and density, is obtained. To quantitatively consider the influence of particle gradation, the coarse-to-fine content (C/F) concept is proposed in this study. The simple relations between the model parameters and particle size, C/F and density are formulated, which enable us to predict the mechanical properties of prototype materials from laboratory tests. Subsequently, a 3D finite element analysis of the Nam Ngum 2 concrete face slab rockfill dam at the end of the construction stage is carried out using two sets of model parameters (1) based on the laboratory tests and (2) in accordance with the proposed method. Comparisons of the computed results with dam monitoring data indicate that the proposed method can provide a simple but effective framework to take account of the scaling effect in dam deformation analysis.

Effects of Maturing Stage of Corn Hybrids on Silage Yield, Feeding Value for Dairy Cows and Milk Production in a Cold Region of Japan

  • Oshita, Tomoko;Takayama, Hideki;Otsuka, Hiroshi;Igarashi, Hiroaki;Nonaka, Kazuhisa;Kume, Shinichi
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.4
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    • pp.511-516
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    • 2007
  • This experiment was conducted to evaluate the effects of differently maturing corn hybrids on silage production and milk production per unit area in the northern part of Japan, where grain development occurs under decreasing ambient temperature. Both hybrids were harvested at the same time. The stages of maturity for the early-maturing hybrids (EH; 80 d relative maturity) and the mid-maturing hybrids (MH; 93 d relative day) were early dent and late dough stage, respectively. The plant yields for MH were higher than those for EH. The dry matter (DM) content of MH was lower than that for EH, and the effluent loss for MH silage was greater than that for EH silage. Therefore, the DM yields of prepared silage per area were similar for both treatments. Twelve multiparous mid-lactation Holstein cows ($58{\pm}13$ days in milk) were fed diets based on EH or MH silage in a crossover design with two 3-week periods. Cows were fed 3 kg of hay crop silage (DM basis) and either EH or MH silage ad libitum, and concentrates were supplied to meet NRC requirement for dairy cows. Silage DM intake for EH was found to be higher (p<0.05) than that for MH (10.0 vs. 9.1 kg/day). Milk production and milk composition for EH were similar to those for MH. Feed efficiency per total feed intake was similar in both treatments, although the feed efficiency per concentrate intake tended to be higher for the EH than that for the MH diet. These results indicate that differences in maturation in corn hybrids affect the effluent production of silage and the silage intake of dairy cows. It may be advantageous to plant early hybrid corn with a reduction in effluent production of silage as well as a reduction in purchased feed costs for dairy cows under the climatic conditions of the northern part of Japan.

Performance and Carcass Composition of Broilers under Heat Stress : I. The Effects of Dietary Energy and Protein

  • Al-Batshan, H.A.;Hussein, E.O.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.6
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    • pp.914-922
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    • 1999
  • An experiment was conducted to determine the effects of dietary energy and protein on performance and carcass composition of broilers under heat stress during the growing period (3-6 weeks). A factorial arrangement of three levels of energy (3.0, 3.2 and 3.4 kcal/g), three levels of protein (18, 20 and 22%), an two rearing temperatures were used in this study. Groups of birds were kept under moderate temperature ($24{\pm}1^{\circ}C/24h$) or hot cycling temperature ($26-34^{\circ}C/6h$, $34{\pm}1^{\circ}C/12h$, and $34-26^{\circ}C/6h$). Body weight (BW), weight gain (WG), feed intake (FI), feed conversion (feed : gain) (FC), carcass weight (CW), carcass yield (YP), breast meat (BM), abdominal fat (AF), drumsticks (DS), and thighs (TH) percentages were determined at the end of the experiment. Hot cycling temperature significantly (p<0.05) decreased BW, WG, FI, CW, and BM, increased FC, YP, DS, and TH but did not affect AF. High energy significantly (p<0.05) increased BW, WG, CW, YP, AF, and TH, decreased FI and FC but did not affect BM or DS. This improvement was observed only under moderate temperature resulting in significant (p<0.05) energy by temperature interaction. High protein significantly (p<0.05) increased BW, WG, CW and BM, decreased AF but did not affect FI, FC, TP, DS, or TH. There were no significant protein by temperature interactions for any of the parameters tested except CW. It is concluded, under the conditions imposed in this experiment, that increasing dietary energy did not alleviate the depressing effect of heat stress while increasing dietary protein up to 22% improved the performance of broilers irrespective of rearing temperature.

Grafting of MMA onto MCC through free radical method and its application to all natural cellulose composite film preparation (Microcrystalline cellulose에 자유 라디칼을 이용한 methyl methacrylate의 그래프팅 반응과 이를 이용한 천연복합필름의 제조)

  • Lee, Soo;Park, Sang-Hee;Jin, Seok-Hwan;Lee, Sun-Young
    • Journal of the Korean Applied Science and Technology
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    • v.25 no.4
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    • pp.459-468
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    • 2008
  • Methyl methacrylate(MMA) was grafted onto microcrystalline cellulose(MCC) with ceric ammonium nitrate(CAN) as a redox initiator at the various conditions. The cellulose triacetate(CTA) composite films added MCC and MMA-grafted MCC powders were prepared on a glass plate. The graft yield(GY) and graft efficiency(GE) of the grafted MCC were calculated with the simple equations by the weight balance method. The double bond of C=O on the grafted MCC surfaces was confirmed by the fourier transform infrared spectroscopy with attenuated total reflection(FT-IT ATR) spectrophotometer. After grafting, the degree of crystallinity of cellulose powders was decresed by judging from x-ray diffraction(XRD) data. Scanning electron microscope(SEM) photos showed the only solvent and CAN solution could change the roughness of MCC powders and the effect of powder dispersions in composite matrix. The tensile strength of MCC/CTA composite films was decreased with increase of MCC powder contents. When 5% grafted MCC was added, the tensile strength of grafted MCC/CTA composite films was increased from 82.3 MPa to 97.2 MPa. The thermal property of powders was also analyzed by the thermogravimetric analysis(TGA).