• Title/Summary/Keyword: yield conditions

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Synthesis of Maltosyl-$\beta$-Cyclodextrin through the Reverse Reaction of Pullulanase (Pullulanase의 Reverse Reaction을 이용한 Maltosyl-$\beta$-Cyclodextrin의 합성)

  • 한일근;이용현
    • Microbiology and Biotechnology Letters
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    • v.19 no.5
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    • pp.444-449
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    • 1991
  • Synthesis of maltosyl-$\beta$-cyclodextrin using maltose ($G_2$) and $\beta$-cyclodextrin ($\beta$-CD) as substrates through the reverse reaction of pullulanase was investigated. The optimal conditions for the condensation reaction were as below: mixing ratio of maltose to $\beta$-CD of 12.7, mixed substrate concentration of 70% (w/w, 70 g/100 ml $H_2O$), and amount of pullulanse of 350 units/100 ml. The concentration of synthesized maltosyl-P-CD concentration was reached up to 2.31 g/100 rnl at above reaction conditions, which corresponded the conversion yield of 43% (w/w, g of branched-CD/g of CD). The synthesis of maltosyl-$\alpha >\gamma >\beta$-CD was also attempted, and conversion yield was in the order of a>y>J3-CDs. Condensation reaction between various maltooligosaccharides ($G-1\sim G_6$ showed that maltose was the most effective oligorner for condensation reaction with $\beta$-CD. To increase the conversion yield various alcohols were added into the reaction mixture, amyl alcohol was found to be the most acceptable alcohol for increasement of convesion yield which increased from 43.0 to 83.0% upon addition of same volume of amyl alcohol into the reaction mixture.

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Processing Optimization of Gelatin from Rockfish Skin Based on Yield

  • Kim, Hyung-Jun;Yoon, Min-Seok;Park, Kwon-Hyun;Shin, Joon-Ho;Heu, Min-Soo;Kim, Jin-Soo
    • Fisheries and Aquatic Sciences
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    • v.13 no.1
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    • pp.1-11
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    • 2010
  • The study was performed to optimize the processing conditions (alkali concentration, extraction time, and temperature) for rockfish skin gelatin based on yield using response surface methodology and comparison of the physicochemical properties with those of rockfish skin gelatin pretreated and extracted under ordinary conditions (alkali treatment concentration: 1.0 M; extraction time: 2 hr; extraction temperature: $60^{\circ}C$). Predicted maximum gelatin yield of 19.1% and gelatin content of 87.8% were obtained by extraction at $106.6^{\circ}C$ for 69.0 min after pretreatment with 1.1 M calcium hydroxide. Yield of gelatin extracted under high temperature/high pressure (G-HT/HP) was 54% higher than that extracted under ordinary temperature/time (G-OT/T). However, G-HT/HP was inferior in gel strength and gelling point to (G-OT/T), but comparable in transmission. Based on the physicochemical properties, G-HT/HP was unsuitable for use in products requiring higher physical properties, but could be useful for health-functional foods.

Factors Influencing Siderophore Production by Plant Growth Promoting Rhizopseudomonas Strains (식물생장촉진 근권 Pseudomonas의 Siderophore 생성에 관여하는 요인)

  • Seong, Ki-Young
    • Korean Journal of Soil Science and Fertilizer
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    • v.28 no.3
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    • pp.287-294
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    • 1995
  • This study demonstrates the influence of environmental conditions, other than iron, on pyoverdin production by fluorescent Pseudomosonas. In slightly acidic pH conditions(pH 6), cell yield was reduced while the siderophore production per cell yield was increased. The optimum temperatures for the siderophore production and cell yield was $19^{\circ}C$ and $28^{\circ}C$ for 7NSK2 and $12^{\circ}C$ and $19^{\circ}C$ for ANP15. The carbon and nitrogen balance showed that at low C : N ratio of the growth medium (higher nitrogen concentration), both cell yield and siderophore production was reduced. Use of different carbon sources revealed that citrate as a carbon source facilitated iron uptake and resulted in a significant reduction in siderophore production. However, at the late exponential phase, the iron content in the cell biomass was not significantly different from those grown in glucose or succinate. From these results it can be suggested that the environmental factors other than iron may also influence siderophore production by fluorescent pseudomonas.

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Morphology, Morphometry, Growth Performance and Carcass Characteristics of Pekin, Nageswari and Their F1 Crossbred Ducks under Intensive Management

  • Ahmad, Md. Tanvir;Nandita, Drishti;Maruf, Tanvir Mohammad;Pabitra, Mohammad Hasanuzzaman;Mony, Sabrina Islam;Ali, Md. Shawkat;Ahmed, Md. Sarwar;Bhuiyan, Mohammad Shamsul Alam
    • Korean Journal of Poultry Science
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    • v.48 no.2
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    • pp.59-67
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    • 2021
  • This study investigated the morphological features, growth, and meat yield performance of Pekin (P), Nageswari (N), and their reciprocal F1 crossbreds (P♂×N♀ and N♂×P♀). A total of 301-day-old ducklings were reared in four different pens up to 20 weeks of age under intensive management conditions. Feeding and management practices were similar for all individuals throughout the experimental period. The morphology and plumage pattern of F1 crossbreds were similar to those of indigenous Nageswari ducks because of the dominant inheritance of the extended Black allele (E locus). Genotype had significant differences (P<0.05) among the four genotypes in morphometric measurements, except wing and shank length. Growth performance was highly significant among the four genotypes (P<0.001) from one-day to 12 weeks of age. The average live weights of P, N, P♂×N♀ and N♂×P♀ crossbred genotypes at 12 weeks of age were 2038.35±29.74, 1542.44±33.61, 1851.85±28.59 and 1691.08±27.80 g, respectively. Meat yield parameters varied significantly (P<0.05) among the different genotypes for all studied traits, except for liver and gizzard weight. Moreover, no significant differences (P>0.05) were observed between P and P♂×N♀ crossbred for important meat yield traits such as hot carcass weight, dressing%, back half weight, drumstick with thigh weight and breast meat weight. Remarkably, the P♂×N♀ crossbreed possesses 50% native inheritance, which contributes to better adaptation in a hot-humid environment. Our results revealed that the P♂×N♀ genotype could be suitable for higher meat production with better adaptability in the agro-climatic conditions of Bangladesh.

Pathway enrichment and protein interaction network analysis for milk yield, fat yield and age at first calving in a Thai multibreed dairy population

  • Laodim, Thawee;Elzo, Mauricio A.;Koonawootrittriron, Skorn;Suwanasopee, Thanathip;Jattawa, Danai
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.4
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    • pp.508-518
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    • 2019
  • Objective: This research aimed to determine biological pathways and protein-protein interaction (PPI) networks for 305-d milk yield (MY), 305-d fat yield (FY), and age at first calving (AFC) in the Thai multibreed dairy population. Methods: Genotypic information contained 75,776 imputed and actual single nucleotide polymorphisms (SNP) from 2,661 animals. Single-step genomic best linear unbiased predictions were utilized to estimate SNP genetic variances for MY, FY, and AFC. Fixed effects included herd-year-season, breed regression and heterosis regression effects. Random effects were animal additive genetic and residual. Individual SNP explaining at least 0.001% of the genetic variance for each trait were used to identify nearby genes in the National Center for Biotechnology Information database. Pathway enrichment analysis was performed. The PPI of genes were identified and visualized of the PPI network. Results: Identified genes were involved in 16 enriched pathways related to MY, FY, and AFC. Most genes had two or more connections with other genes in the PPI network. Genes associated with MY, FY, and AFC based on the biological pathways and PPI were primarily involved in cellular processes. The percent of the genetic variance explained by genes in enriched pathways (303) was 2.63% for MY, 2.59% for FY, and 2.49% for AFC. Genes in the PPI network (265) explained 2.28% of the genetic variance for MY, 2.26% for FY, and 2.12% for AFC. Conclusion: These sets of SNP associated with genes in the set enriched pathways and the PPI network could be used as genomic selection targets in the Thai multibreed dairy population. This study should be continued both in this and other populations subject to a variety of environmental conditions because predicted SNP values will likely differ across populations subject to different environmental conditions and changes over time.

Moment redistribution of RC continuous beams: Re-examination of code provisions

  • Da Luo;Zhongwen Zhang;Bing Li
    • Structural Engineering and Mechanics
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    • v.85 no.5
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    • pp.679-691
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    • 2023
  • Many codes allow designers to use the bending moment diagram computed by elastic analysis and modify it by a certain amount of moment redistribution (MR) to account for plastic behaviour of continuous beams. However, several researchers indicated that the MR at the ultimate limit state (𝛽u) for some beams deviate significantly from the specified values of various codes. This paper examines the applicability of the provisions on 𝛽u in ACI 318-19 and Eurocode 2 through numerical investigations and comprehensively explores the influencing factors. The results show that some parameters not considered in those codes influence 𝛽u to a certain extent, where the ratio of tensile reinforcement ratio at intermediate support to tensile reinforcement ratio at midspan (𝜌s1/𝜌s2) and load type are crucial parameters to consider. The specific combination of these two parameters may make the codes overestimate or significantly underestimate the 𝛽u. On the other hand, the yield state of both critical sections is found to have an important influence on the influence degree of each parameter on 𝛽u. The yield conditions are investigated, and an empirical judgment equation is proposed. In addition, the influence laws of the critical parameters on 𝛽u have been further proved by theoretical derivation. Finally, due to 𝜀t is found to have a better linear correlation with 𝛽u than xu/d, equations as a function of 𝜀t for predicting the 𝛽u of continuous beams under the two loads are proposed, respectively.

The Effect of Phosphorus, Potassium and Calcium Application on Root Activity and Grain Yield of Paddy Rice on Different Soil Conditions (토양 조건별 인산, 가리 및 석회 시용이 수도의 근활력 및 수량에 미치는 영향)

  • Si-Yung Ahn
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.22 no.1
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    • pp.110-134
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    • 1977
  • Effects of phosphorus, potassium and calcium applications on root activity and agronomic characters of paddy rice were studied in submerged paddy soil on which starch was applied to accelerate soil reduction and production of toxic substance during 1971~1973. Root activity at heading date was decreased in reduction accelerated soil by starch application. Effect of calcium application on root activity was differed along soil conditions, phosphorus or potassium levels. Single effects of phosphorus or potassium application were not significant on every observed characters. Calcium application when phosphorus or potassium was not used as basal but used as top dressing increased yield and yield components, especially filled grain ratio. Effect of calcium application along soil conditions was differed on agronomic characters, but calcium application with phosphorus or potassium was more effective to increase yield components and grain yield. Sufficient application of phosphorus, potassium and calcium could improve the nutritional situation of rice plant for panicle development and grain maturity in reduction accelerated paddy soil in which poor growing of rice plant after panicle formation is anticipated by the production of toxic substance from soil.

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Synthesis of Trehalose by Pleurotus spp. -Cultural conditions­- (느타리버섯균의 Trehalose 합성(合成)(I) -배양(培養) 조건(條件)-)

  • Hong, Jai-Sik;Yun, Se-Eok;Kim, Young-Soo;Lee, Jong-Bae
    • The Korean Journal of Mycology
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    • v.15 no.2
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    • pp.108-115
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    • 1987
  • Nutritional characteristics and cultural conditions for trehalose synthesis and mycelial yield of Pleurotus sajor-caju and Pleurotus ostreatus 201 in submerged culture were investigated. The results were as follows: Among the carbon sources, glucose was most excellent for trehalose synthesis and mycelial yield. The optimal concentration of glucose was 1%. Among the nitrogen sources, peptone was most excellent for trehalose synthesis and mycelial yield. The optimal concentration of peptone was 0.05%. The optimal concentration of $KH_2PO_4$ and $MgSO_47H_2O$ for trehalose synthesis and mycelial yield was 0.1%,0.04% and 0.2%,0.04-0.08%, respective­ly. The optimal temperature and pH for trehalose synthesis were $25^{\circ}C$ and pH 5.5. but optimal temperature and pH for mycelial yield were $30^{\circ}C$ and pH 5.5. The maximum yield of trehalose was obtanined after 10 day cultivation.

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Extraction Yield and Anti-Yeast Activity of Extract from Green Tea Seeds by Pretreatment and Extraction Conditions (녹차씨 전처리와 추출 조건에 따른 녹차씨 추출물의 추출 수율 및 항효모 활성)

  • Yang, Eun Ju;Seon, Yoo Kyung;Wee, Ji-Hyang
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.9
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    • pp.1351-1357
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    • 2016
  • Green tea (Camellia sinensis) seed extract (GTSE) was prepared under various pretreatment conditions and used to investigate its extraction yield and anti-yeast activity. Anti-yeast activity of GTSE from seeds with or without the coats was the same, whereas the extraction yield was slightly higher in extract from seeds without the coat. Anti-yeast activity of GTSE from seeds with different water contents or particle sizes was the same, whereas the extraction yield was highest in extract from seeds with 7.3% moisture or a smaller particle size. Anti-yeast activity of defatted green tea seed extract (DGTSE) was the same as that of GTSE. Extraction yield was higher in DGTSE from defatted seeds by the oil press machine compared to hexane extraction. Defatted green tea seed (DGTS), a by-product from the oil extraction process, is a good natural source of anti-yeast preservative. The extraction yield and anti-yeast activity of DGTSE were investigated using various extraction solvents, temperatures, and times. The results show that water was an economic extraction solvent, and anti-yeast activity of DGTSE was unstable at $90^{\circ}C$. These results suggest that water, extraction temperature of $50^{\circ}C$, and extraction time of 4 h were the most efficient for extraction of anti-yeast compounds from DGTS.

A Description of Thermomechanical Behavior Using a Rheological Model (리올러지 모델을 이용한 열적 기계적 변형 거동 모사)

  • Lee Keum-Oh;Hong Seong-Gu;Lee Soon-Bok
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.30 no.7 s.250
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    • pp.757-764
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    • 2006
  • Isothermal cyclic stress-strain deformation and thermomechanical deformation (TMD) of 429EM stainless steel were analyzed using a rheological model employing a bi-linear model. The proposed model was composed of three parameters: elastic modulus, yield stress and tangent modulus. Monotonic stress-strain curves at various temperatures were used to construct the model. The yield stress in the model was nearly same as 0.2% offset yield stress. Hardening relation factor, m, was proposed to relate cyclic hardening to kinematic hardening. Isothermal cyclic stress-strain deformation could be described well by the proposed model. The model was extended to describe TMD. The results revealed that the hi-linear thermomechanical model overestimates the experimental data under both in-phase and out-of-phase conditions in the temperature range of $350-500^{\circ}C$ and it was due to the enhanced dynamic recovery effect.