• Title/Summary/Keyword: yield conditions

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Screening and Characterization of Flocculent Yeast, Candida sp. HY200, for the Production of Xylitol from D-Xylose

  • KANG HEUI YUN;KIM YONG SUNG;KIM GEUN JOONG;SEO JIN HO;RYU YEON WOO
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.362-367
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    • 2005
  • On the basis of high osmotic tolerance and xylitol production, a novel yeast strain was screened from soils of rice farming. The isolated strain HY200 was systematically characterized by using general approaches of Biolog Microlog$^{TM}$ and 18S rRNA sequence analyses, and consequently was designated as Candida tropicalis HY200. Under formulated culture conditions, relatively high xylitol yield ($77\%$) and productivity (2.57 g/l$\codt$h) were obtained, in practice, when 200 g/l of xylose was supplemented. In the utilization of nitrogen, inorganic compounds could not serve as nitrogen sources. As a promising phenotype, HY200 steadily flocculated during and/or after growing in the formulated medium. The extent of flocculation was partly affected by nitrogen sources. However, regardless of the kinds of carbon source fed, the flocculent cells were always observed at the end of the exponential growth phase. These observations strongly suggest that the strain HY200 could effectively be used as a potential candidate for the production of xylitol from xylose, especially in repeated batch mode, because of its flocculation ability and tolerance to high substrate concentrations.

Alcohol Productivity Using Starchy Raw Material in Pilot Scale Multi-stage CSTR (Pilot Scale Multi-stage CSTR에서 전분질 원료를 이용한 알콜 생산)

  • Nam, Ki-Du;Lee, In-Ki;Cho, Hoon-Ho;Kim, Woon-Sik;Suh, Kuen-Hack;Ryu, Beung-Ho
    • Microbiology and Biotechnology Letters
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    • v.22 no.1
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    • pp.80-84
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    • 1994
  • In order to induce the rapid alcohol fermentation through the increases of the cell density in a continuous alcohol fermentation of naked barley, the single-cultivation with S. cerevisiae IS-019(SCM, ordinary control), mixed-cultivation with Saccharomyces uvarum IS-026 having a flocculent ability and S. cerevisiae IS-019(MCM), and mash recirculation by single-cultivation of S. cerevisiae IS-019(MRM) modes were investigated. The cell mass in the mixed-cultivation mode was about 10% higher than that of ordinary control but the final alcohol yield was slightlyl decreased. When recycled the mash with the flow rate of 7 l/h from V$_{6}$ to V$_{5}$ fermentors under the ordinary control, the cell density was distributed at 140~170$\times $10$^{6}$ cell/ml depending upon the fermentorsorders, higher about 20% than that of the ordinary control. Under these conditions the alcohol productivity of the maximum and the overall was 12.16 g/l$\cdot $h with an alcohol of 7.6% at the V$_{5}$ fermentor and 1.19 g/l$\cdot $h with an alcohol of 8.94%, respectively. For higher cell mass it was more effective to apply the mash recirculation mode with the single-cultivation of S. cerevisiae IS-019 in a pilot scale multi-stage CSTR.

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Legal Aspects for Quality Evaluation Standard of Plant Variety and Seed (식물 품종 및 종자의 품질평가 기준에 대한 규정현황)

  • 최근진
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47
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    • pp.200-215
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    • 2002
  • There are some international standards and organization controling the quality characteristics of varieties and seeds such as UPOV, OECD, ISTA, AOSA. The National List system to check variety performance such as quality characteristics of plant varieties and seeds was established by each countries. The UPOV (International Union for the Protection of New varieties of Plant) regulates the requirement of plant variety protection that is distinctness (D), uniformity (U), stability (S) (here in after called DUS), Novelty and Denomination, and some quality characteristics are used to examine the requirement of plant variety protection by each crops. OECD (Organization for Economic Cooperation Development) seed scheme regulates the seed certification for seed trade between countries. ISTA (International Seed Testing Association) and AOSA(Association for Official Seed Analyst) regulates the seed analysis standard and methods. Most of the countries in Europe has the National List system, which check the value for cultivation and use(VCU), that is to say, variety performance such as yield, quality and stress to environment. All the seeds should be enlisted in the National List before sell the seed in their country. All the quality characteristics checked variety performance are for instance, amylose and amylopectin content in rice, molting quality, $\beta$-glucan, protein in barley, protein and sugar content in soybean, sugar and amylopectin content in corn etc. Conditions for the protection of new variety of plant are DUS. Quality characteristics may be the important characteristics and used to check DUS in crop by crop. It is very important to develop a new characteristics and establish standard method fur examine the VCU and DUS test for each crop.

Efficient Fault Detection Method for a Degaussing Coil System Based on an Analytical Sensitivity Formula

  • Choi, Nak-Sun;Kim, Dong-Wook;Yang, Chang-Seob;Chung, Hyun-Ju;Kim, Heung-Geun;Kim, Dong-Hun
    • Journal of Magnetics
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    • v.18 no.2
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    • pp.135-141
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    • 2013
  • This paper proposes an efficient fault detection method for onboard degaussing coils which are installed to minimize underwater magnetic fields due to the ferromagnetic hull. To achieve this, the method basically uses field signals measured at specific magnetic treatment facilities instead of time-consuming numerical field solutions in a three-dimensional analysis space. In addition, an analytical design sensitivity formula and the linear property of degaussing coil fields is being exploited for detecting fault coil positions and assessing individual degaussing coil currents. Such peculiar features make it possible to yield fast and accurate results on the fault detection of degaussing coils. For foreseeable fault conditions, the proposed method is tested with a model ship equipped with 20 degaussing coils.

Practical Process Development of AMCA from Recyclable Source MFB (재사용이 가능한 MFB로부터 AMCA의 합성 공정 개발)

  • Kim, Jong Cheon;Ryu, Young;Kim, Seok Chan
    • Applied Chemistry for Engineering
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    • v.27 no.5
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    • pp.508-511
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    • 2016
  • A facile synthesis of 4-(aminomethyl)cyclohexanecarboxylic acid (AMCA) from recyclable methyl 4-formylbenzoate (MFB) was described. In particular, we investigated the best process variables such as catalyst, hydrogen pressure, reaction temperature, and reaction time for AMCA conversion from AMBA through hydrogenation reaction. The best conditions were found to be as follows: the catalyst as [5% Pd/C, 5% Pt/C, 5% Rh/C (1 : 1 : 1)] which is a composite catalyst, the pressure as 30 bar, the reaction time as 8 hours and the reaction temperature at $130^{\circ}C$. Under the condition, a 90% yield (purity 99.9%) for the mass production of AMCA was achieved.

Longitudinal and Vertical Variations of Long-term Water Quality along with Annual Patterns in Daecheong Reservoir

  • Lee, Sang-Jae;Shin, Jae-Ki;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.43 no.2
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    • pp.199-211
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    • 2010
  • The objectives for this study were to evaluate spatial and temporal characteristics of water quality, based on long-term water quality monitoring data during 1993~2008. We found that physico-chemical and ecological conditions in the Daecheong Reservoir (DR) were modified by the construction of upper dam (i.e., Yongdam Reservoir). total phosphorus (TP), Secchi depth (SD), and chlorophyll-a (CHL) in the DR showed significant longitudinal decreases along the headwater-to-the downlake, indicating a large spatial variation, and this gradient was more intensified during the high-flow season (monsoon). Nutrient-rich water containing high nitrogen and phosphorus in the monsoon season (July~August) passed through the reservoir as a density current in the metalimnetic depth, and also high suspended solids increased in the metalimnetic depth, especially during the monsoon. According to the deviation analysis of Trophic State Index (TSI), >50% of TSI (CHL)-TSI (SD) and TSI (CHL)-TSI (TP) values were negatives, so that inorganic suspended solids (non-votatile solids) influenced the underwater light regime against phytoplankton growth. Also, ratios of CHL:TP after the dam construction evidently increased, compared to the values before the upper dam constructions, indicating a greater yield of phytoplankton in the unit phosphorus. Overall data showed that ecological and functional changes in Daecheong Reservoir occurred after the construction of upper dam (Yongdam Reservoir).

Systemic Statistical Optimization of Astaxanthin Inducing Methods in Haematococcus pluvialis cells -Statistical Optimization of Astaxanthin Production in Haematococcus

  • Kim, Sun-Hyoung;Jeong, Sung Eun;Hong, Seong-Joo;Lee, Choul-Gyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.6 no.1
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    • pp.31-40
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    • 2014
  • The production of astaxanthin in the microalga Haematococcus pluvialis has been investigated using a sequential methodology based on the application of two types of statistical designs. The employed preliminary experiment was a fractional factorial design $2^6$ in which the factors studied were: excessive irradiance and nitrate starvation, phosphate deficiency, acetate supplementation, salt stress, and elevated temperature. The experimental results indicate that the amount of astaxanthin accumulation in the cells can be enhanced by excessive irradiance and nitrate starvation whereas the other factors tested did not yield any enhancement. In the subsequent experiment, a central composite design was applied with four variables, light intensity, nitrate, phosphate, and acetate, at five levels each. The optimal conditions for the highest astaxanthin production were found to be $1040{\mu}E/(m^2{\cdot}s)$ light intensity, 0.04 g/L nitrate, 0.31 g/L phosphate, 0.05 g/L acetate concentration.

Optimization of Biodiesel Production from Waste Frying Oil using Response Surface Method (Response Surface Method를 이용한 폐식용유로부터 바이오디젤 생산의 최적화)

  • 이세진;김의용
    • KSBB Journal
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    • v.17 no.4
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    • pp.396-402
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    • 2002
  • Biodiesel has attracted considerable attention during the past decade as a biodegradable, nontoxic, and renewable fuel, Several processes for the production of biodiesel have been developed, among which transesterification under alkali-catalysis gives high level yield of methyl esters in short reaction times. In this research, response surface method was applied to optimize the transesterification reaction under alkali-catalysis. It was found that reaction temperature, reaction time, and agitation rate of reactor had profound effects among the seven variables affecting on biodiesel conversion. The optimal temperature, reaction time, and agitation speed were 67$^{\circ}C$, 68 minutes, and 94 rpm, respectively. Under the optimal conditions, the experimental value of biodiesel conversion was 99.7%.

The Separation and Recovery of Nickel and Lithium from the Sulfate Leach Liquor of Spent Lithium Ion Batteries using PC-88A

  • Nguyen, Viet Tu;Lee, Jae-Chun;Jeong, Jinki;Kim, Byung-Su;Pandey, B.D.
    • Korean Chemical Engineering Research
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    • v.53 no.2
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    • pp.137-144
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    • 2015
  • The present paper deals with the extractive separation and selective recovery of nickel and lithium from the sulfate leachate of cathode scrap generated during the manufacture of LIBs. The conditions for extraction, scrubbing and stripping of nickel from lithium were optimized with an aqueous feed containing $2.54kg{\cdot}m^{-3}$ Ni and $4.82kg{\cdot}m^{-3}$ Li using PC-88A. Over 99.6% nickel was extracted with $0.15kmol{\cdot}m^{-3}$ PC-88A in two counter-current stages at O/A=1 and pH=6.5. Effective scrubbing Li from loaded organic was systematically studied with a dilute $Na_2CO_3$ solution ($0.10kmol{\cdot}m^{-3}$). The McCabe-Thiele diagram suggests two counter-current scrubbing stages are required at O/A=2/3 to yield lithium-scrubbing efficiency of 99.6%. The proposed process showed advantages of simplicity, and high purity (99.9%) nickel sulfate recovery along with lithium to ensure the complete recycling of the waste from LIBs manufacturing process.

Accelerated the environmental stress cracking (ESC) study of polymer materials using a plastic ruler and a PVC eraser (플라스틱 자와 지우개를 이용한 고분자재료의 환경응력파괴(ESC) 가속실험 방법에 관한 연구)

  • Park, Joon Hyung;Ahn, Won Bae;Yoo, Jin Seong;Kim, Kyoung Mun;Nam, Kyoung Hyun
    • Journal of Applied Reliability
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    • v.13 no.2
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    • pp.141-151
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    • 2013
  • The study of environmental stress cracking (ESC) by contact with plastic rulers and PVC erasers which was artificially added to the external stress was carried out in order to accelerate the chemicals crack. To analyze a blooming plasticizer that migrates from the inside of blended eraser to the surface of the eraser, the ESC experiments were carried out at room temperature and $50^{\circ}C$ conditions. The chemicals crack shape caused by the plasticizer and the brittle fracture shape resulted from the external stress were observed in consequence with the cross-sectional surface analysis of the ruler crack by the ESC. The bending strength of the plastic rulers were fractured prior to the yield point and it had low bending flexure stress. We presented that ESC of polymer materials was affected by the polarity of the chemicals and polymer, the exposure time to chemicals, the exposure temperature and the level of strain on the polymer.