• The Journal of Korean Medicine
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• v.30 no.4
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• pp.1-12
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• 2009

Objectives: This study was to investigate the anti-oxidation and anti-cancer activity of Chaga mushroom extract. Extraction condition optimization and beta-glucan analysis and anti-cancer activity tests were also done. Methods: Optimum extraction conditions for Chaga mushroom extract were at a temperature of $90^{\circ}C$ and 2hrs with 10 times of water. Extraction yield and economics were best under these conditions. Results: Anti-oxidation activity was the highest with the fraction of 100,000 MWCO and $IC_{50}$ value was $13{\mu}g/ml$ and this value was comparable to that of vitamin E, alpha-tocopherol. Among the fractions from various organic solvents, ethyl acetate fraction showed the highest anti-oxidation activity with $IC_{50}$ value of $7{\mu}g/ml$. For anti-cancer activity, chloroform fraction showed little anti-cancer activity and ethyl acetate fraction showed the best anti-cancer activity with $IC_{50}$ $1.5{\mu}g/ml$ for stomach cancer cells. Anti-cancer activities for different molecular weight fractions were the best in the fraction of molecular weight less than 100,000Da, and $IC_{50}$ values for stomach cancer cells and liver cancer cells were 1.7 and $1.4{\mu}g/ml$, respectively. Conclusions: From these results, we can conclude that the extract of Chaga mushroom could be a good source for functional food and natural anti-cancer medicine.

• Microbiology and Biotechnology Letters
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• v.5 no.3
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• pp.119-125
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• 1977

The cultivation of C. flavigena KIST 321, capable of utilizing cellulosic resources, was carried out in a 500 L fermentor by the batch process and the productivities of cellulosic SCP have been investigated by establishing the optimal conditions and levels of cellulosic material and others as medium components. The highest yield of the cell mass in the batch process was atttained under tile conditions at 30$^{\circ}C$, pH 7.4, 0.4∼0.6 VVM of aeration and at 130 rpm of agitation. According to the material balance of cellulosic SCP production using tile pretreated rice straw as a carbon source, more than 25 percent of rice straw on the base of drying weight was recovered in the form of cell mass.

• Microbiology and Biotechnology Letters
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• v.17 no.3
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• pp.224-230
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• 1989

For the production of catechol from benzene, bacteria capable of assimilating benzene as a sole carbon and energy source were isolated from soils. Among them, newly isolated strain, KY-114 hay-ing the best ability of producing catechol from benzene was selected and a mutant Pseudomonas sp. HW-103 was developed from Pseudomonas sp. KY-114 by using mutagenesis induced by N-methyl - N'- nitro - N -nitrobo guanidine. The catechol reduction from benzone by Pseudomonas sp. HW-103 was investigated under various conditions. The highest catechol concentration (0.61 g/$\ell$) was obtained in the growth medium (pH 6.5) containing 1% sodium citrate, 0.75% (NH$_4$)$_2$SO$_4$, 0.15% benzene and other minerals at 3$0^{\circ}C$ after incubating of 15hrs. In the catechol production through the reaction with resting rolls, 2.5 g/1 of catechol was produced from 4 g/$\ell$ of benzene after incubation of 10 hrs under the optium conditions, which correponds to 45% of theoretical catechol yield.

• Journal of the Korean Wood Science and Technology
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• v.44 no.4
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• pp.477-493
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• 2016

A single process using hot water (0% green liquor) and green liquor (GL) was investigated for pre-pulping extraction on two types of raw material. The GL was applied at different alkali charges of 0-5% on a dry wood weight basis. The extractions were performed at an H-factor 900 at $180^{\circ}C$. The 0% and 3% GL extraction detected acetic acid (AA) at 10.02 and $9.94g/{\ell}$, extracts derived from hardwood, 2.46 and $3.76g/{\ell}$, extracts derived from softwood, respectively. The single liquid-liquid extraction (LLE) was studied using tri-n-alkylphosphine oxide (TAPO). Response surface methodology (RSM) was employed as an efficient approach for predictive model building and optimization of AA recovery conditions. The extraction of AA was evaluated with a three-level factorial design. Three independent variables, pH (0.5-3.5), temperature ($25-65^{\circ}C$), and residence time (24-48 min) were investigated. Applying the RSM models obtained, the optimal conditions selected of extracts derived from hard- and softwood with a 3% GL were approximately pH 1.4, $26.6^{\circ}C$, 43.8 min and approximately pH 0.7, $25.2^{\circ}C$, 24.6 min, respectively. The predicted and experimental values of AA recovery yield were similar whilst sugar retention was 100%.

• Journal of Mushroom
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• v.9 no.2
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• pp.80-83
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• 2011

Sparassis crispa(Cauliflower mushroom) was an edible mushroom that shows remarkably high contents of ${\beta}$-glucan compared to other edible mushroom. In this study, we aimed to select suitable conditions and materials for mycelial growth and fruit body production. The longer saccharification time of barely result in higher sugar content for eight hours. The optimal sugar content of media for mycelial growth was showed at level of 6 $^{\circ}Brix$. Optimum temperature and pH for mycelial growth in liquid spawn were $25^{\circ}C$ and pH 5.0~6.0, respectively. In case of sawdust was used Larix kaempferi as main material, the fruit body yield and cultivation period of supplemented with 10% wheat flour and 20% corn flour were highest and fastest, respectively.

• Journal of Pharmaceutical Investigation
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• v.26 no.3
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• pp.221-232
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• 1996

An abrasive, calcium hydrogen phosphate dihydrate (DCPD), was synthesized in a Box-wilson experimental design by reactions between phosphoric acid and milk of lime, and calcium chloride and sodium phosphate solutions, and stabilized with TSPP and TMP. The optimum conditions for preparation of DCPD from phosphoric acid with milk of lime were such as; reaction temp.; $51.9^{\circ}C$, conc. of lime; 25.9%, conc. of phosphoric acd; 77.9%, drying temp.; $60.2^{\circ}C$ and final pH; 6.46. The physico-chemical and pharmaceutical properties of DCPD were showed as follows: glycerin absorption value(68 ml/100g), whiteness(99.5%), particle size(10.9 nm), pH(7.8), and set test(pass). XRD and SEM of DCPD indicated a monoclinic system crystallographically. $N_2$ adsorption isotherm curve by BET showed non porous type II form. The micromeritic parameters of DCPD showed that surface area was $3.27{\sim}4.6\;cm^{2}/g$ and pore volume, pore area and pore radius were negligible. The rheogram of the toothpaste containing DCPD showed pseudoplastic flow with yield value of 321, and thixotropic behavior forming hysteresis loop. These results meet the requirements as abrasive standard, and sythesized DCPD is expected as a good dental abrasive such as a high quality grade in practice.

• Journal of Bio-Environment Control
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• v.15 no.1
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• pp.107-113
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• 2006

These studies were carried out to investigate the quality of green tea processed by different pan-firing hand rolling and to select the best conditions during the procedure of green tea processing the results were as follows. At harvestion tea plant leaf, tea plants had 4 leaves. each leaf size was 7.5 cm'$\square$2.6 cm, and weight of 100 buds 46.6 g. The increase of pan-fired time decreased in the moisture content and yield of green tea. After processing green tea, 5 times pan-fired one had much more contents of total nitrogen (T-N:5.31%), caffeine (2.00%) and total amino acid (T.A.A. : 3,046 mg%) and less content of tannin (13.41%) than the other treatments. but 3 times pan-fired one had the highest contents of vitamin C (204.3 mg%) and chlorophyll (365.0 mg%) among three kinds pan-firing method. Sixteen kinds of free amino acid including theanine as a good taste component of pan-fired green tea were isolated and measured by HPLC. Free amino acid (FAA) contents were in the range of $2,683{\sim}2,925$ mg% and among various pan-firing methods, 5 tims pan-fired one had the highest content of F.A.A. Among these free amino acids, theanine occupied as much as $56.4{\sim}61.3%$ of F.A.A. Lightness, yellowness and redness of 5 times pan-fired green tea were middle value among three different treatments. Besides, it gained the highest score of sensory evaluation among them. These above results suggested that 5 times pan-fired green tea was considered to be the best level as pan-firing times of green tea processing.

• Korean Journal of Environmental Agriculture
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• v.39 no.2
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• pp.138-141
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• 2020

BACKGROUND: Fresh weight is one of the major quality measurement factors in determining the quality of fresh fruits. A practical method has been developed for rapid and non-destructive measurement using the Chlorophyll Fluorescence Image (CFI) technique to estimate changes in fresh weight of post-harvest products. METHODS AND RESULTS: Kiwifruit (Actinidia deliciosa) was used and measured for the fresh weight and CFI under different temperature conditions at 0, 10, and 20℃, from 0 to 21 days after storage (DAS). We observed the fresh weight of kiwifruit and measured the surface image for determining F_v/F_m value in terms of maximum quantum yield on each day. To estimate freshness of kiwifruit we applied linear regression between the measured fruit weights and F_v/F_m values. Results showed that fruit weights were reduced by 4% at 0℃, 6% at 10℃, and 14% at 20℃ for 21 days, respectively. And also, the value of Fv/Fm was shown as decreasing trend at all temperature conditions, especially at 20 ℃. Fv/Fm values showed highly significant correlation (R²>0.9) with fresh weight of kiwifruit at all different storage temperatures. CONCLUSION: Thus, CFI technique can be useful to estimate the fresh weight of kiwifruit.

• KSBB Journal
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• v.17 no.6
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• pp.543-548
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• 2002

The enantioselective esterification of racemic ibuprofen catalyzed by a Candida rugosa lipase was studied according to reaction conditions such as a lipase concentration, reaction temperature, alcohol chain length and alcohol concentration. The S-(+)-ibuprofen alkyl esters prepared were converted to S-(+)-ibuprofen by hydrolysis with sulfuric acid as a catalyst. High conversions in the esterifications were obtained at 60$^{\circ}C$ and an equimolar ratio of octanol to ibuprofen. The initial reaction rate of the esterification decreased with increasing octanol concentration. Conversion and initial reaction rate increased with increasing alcohol chain length. Values of enantiomeric excess(ee) according to esterification reaction conditions did not change below 60$^{\circ}C$. On the other hand, values of conversion and ee for the chemical hydrolysis of S-(+)-ibuprofen alkyl esters were independent of alcohol alkyl chain length. Optical resolution of racemic ibuprofen was achieved by lipase catalyzed esterification and chemical hydrolysis. The separation method provided a high yield and enantioselectivity for the production of S-(+)-ibuprofen from racemic ibuprofen.

• KSBB Journal
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• v.22 no.4
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• pp.197-200
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• 2007

This study examined the conditions of filtration and concentration of methanol extract from biomass. Filtration efficiency was improved by adding diatomaceous earth as a filter aid. The optimal amount of diatomaceous earth was 6% (w/w) to reduce the filtration time. The filtration time was reduced by 4.2% in first extraction, 30.0% in second extraction, 22.8% in third extraction, and 19.0% in fourth extraction, respectively. The optimal temperature of water bath was below 50$^{\circ}C$ for preventing paclitaxel degradation during concentration of methanol extract using a rotary evaporator. The temperature of concentrated solution in rotary evaporator was relatively low compared to bath temperature because of latent heat of evaporation. The stopping point of concentration in rotary evaporator for the following step was at a specific gravity of 0.96 of the concentrated solution in terms of the purity and yield of paclitaxel. This information is very useful for mass extraction of biomass for the recovery of paclitaxel from plant cell culture.