• 제목/요약/키워드: yield conditions

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Optimization of Epoxide Hydrolase-Catalyzed Enantioselective Hydrolysis of Racemic Styrene Oxide (Rhodotorula sp. CL-83 유래의 에폭사이드 가수분해효소를 이용한 라세믹 Styrene Oxide 입체특이성 가수분해 조건 최적화)

  • 이은열
    • Journal of Life Science
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    • v.12 no.6
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    • pp.765-768
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    • 2002
  • Enantioselective hydrolysis of racemic styrene oxide by Rhodotorula sp. CL-82 was investigated. Reaction conditions including pH, temperature, and volume ratio of organic cosolvent were optimized using response surface methodology, and the optimal conditions of pH, temperature, and the volume ratio of cosolvent were determined to be 7.64, $33.26^{\circ}C$, and 3.09 %(v/v), respectively. Chiral (S)-phenyl oxirane could be obtained with high enantiomeric purity (ee > 99%) and 20% yield (theoretical yield = 50%) at the optimal rendition.

Evaluation of Optimum Conditions for Bacteriocin Production from Lactobacillus sp. JB-42 Isolated from Kimchi

  • Jo, Young Bae;Kyung Mi Bae;Sung Koo Kim;Hong Ki Jun
    • Journal of Microbiology and Biotechnology
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    • v.6 no.1
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    • pp.63-67
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    • 1996
  • Bacteriocin-producing microorganism was isolated from Kimchi. The microorganism was identified as a Lactobacillus sp. and named Lactobacillus sp. JB-42. The optimum conditions for the bacteriocin production from the isolated microorganism were evaluated. For the maximum yield of bacteriocin from Lactobacillus sp. JB-42, the cell should be harvested at the early stationary phase and the temperature, pH and NaCl concentration should be $30^{\circ}C$, pH 7 and without the addition of NaCl, respectively. Sucrose, glucose or fructose should be used as a carbon source and organic nitrogen such as tryptone should be used as a nitrogen source for the best yield. The production of bacteriocin was related to the cell growth of Lactobacillus sp. JB-42 indicating the role of Lactobacillus in the Kimchi fermentation process.

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The Effects of Heat-treatment Conditions and Alloy Compositions on Tensile Properties in Al-Mg Alloys for Automobile Body Panels (차체 판넬용 Al-Mg합금에서 열처리조건 및 조성변화가 인장특성에 미치는 영향)

  • Kang, S.B.;Lim, C.Y.;Kim, H.W.
    • Transactions of the Korean Society of Automotive Engineers
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    • v.2 no.2
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    • pp.95-102
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    • 1994
  • Aluminum sheet application to automobile body panels has now become an important objective to meet the requirements of automobile weight reduction. As the Mg content in Al-Mg based alloys increased up to 7.19%, the strength and elongation increased. For instance. Al-7.19Mg alloy had a high strength of 305MPa and a high elongation of 35%. A study was also made to investigate the interrelation between grain size and tensile properties with varying the contents of Mg, Ti and Zr elements and annealing conditions. The yield stress decreased as the grain size increased, which increased the uniform elongation. The strain hardening exponents n increased as the Mg content increased, which depended on the increasing difficulties of the cross slip of dislocation.

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Kinetics and Optimization of Dimethyl Carbonate Synthesis by Transesterification using Design of Experiment

  • Lee, Kilwoo;Yoo, Kye Sang
    • Korean Chemical Engineering Research
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    • v.56 no.3
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    • pp.416-420
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    • 2018
  • A comprehensive kinetic study has been conducted on dimethyl carbonate synthesis by transesterification reaction of ethylene carbonate with methanol. An alkali base metal (KOH) was used as catalyst in the synthesis of DMC, and its catalytic ability was investigated in terms of kinetics. The experiment was performed in a batch reactor at atmospheric pressure. The reaction orders, the activation energy and the rate constants were determined for both forward and backward reactions. The reaction order for forward and backward reactions was 0.87 and 2.15, and the activation energy was 12.73 and 29.28 kJ/mol, respectively. Using the general factor analysis in the design of experiments, we analyzed the main effects and interactions according to the MeOH/EC, reaction temperature and KOH concentration. DMC yield with various reaction conditions was presented for all ranges using surface and contour plot. Furthermore, the optimal conditions for DMC yield were determined using response surface method.

The Influence of Interphase Condition on Mechanical Properties of Short-Fiber Reinforced Rubber (계면상 조건이 단섬유 강화고무의 기계적 성질에 미치는 영향)

  • Ryu, Sang-Yeol;Lee, Dong-Ju
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.24 no.3 s.174
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    • pp.625-633
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    • 2000
  • The mechanical and curing properties of short nylon66 fiber reinforced Chloroprene rubber have been investigated as functions of interphase conditions and fiber content. The tensile strength exhibits a dilution effect at a low fiber content in each interphase. It is found that the interphase conditions have an important affect on the dilution ratio and critical fiber content. Double coatings of bonding agent 402 and rubber solution become the best interphase model in this study. The yield strength, tensile modulus, tear strength and fracture toughness at rupture, Jr are significantly improved due to fiber concentration.

Effect of Graft Copolymerization Conditions on Grafting Yield of AA and MA onto Polyester Fiber

  • Park, Myung-Ja
    • The International Journal of Costume Culture
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    • v.6 no.2
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    • pp.134-146
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    • 2003
  • Polyester of linear homopolymer poly(ethylene terephthalate)(PET) was chemically modified through the formation of branched copolymer to improve the undesirable properties of fiber. Photo-induced graft copolymerization of the acrylic monomers acrylic acid (AA) and methyl acrylate (MA) in the liquid and vapor phase, respectively, onto N,N-dimetylformamide (DMF)-pretreated PET fibers was carried out. The effect of various synthesis conditions and DMF pretreatment of the PET on the graft yield was investigated. Grafting mechanism was analysed. The grafting was promoted by increasing DMF pretreatment temperature and amount of DMF retention in the fiber. The increasing biacetyl and monomer flow time, and irradiation time enhanced the grafting up to a certain amount and thereafter it decreased.

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Deformation-based Strut-and-Tie Model for flexural members subject to transverse loading

  • Hong, Sung-Gul;Lee, Soo-Gon;Hong, Seongwon;Kang, Thomas H.K.
    • Computers and Concrete
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    • v.18 no.6
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    • pp.1213-1234
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    • 2016
  • This paper describes a deformation-based strut-and-tie model for the flexural members at post-yield state. Boundary deformation conditions by flexural post-yield response are chosen in terms of the flexural bar strains as the main factor influenced on the shear strength. The main purpose of the proposed model is to predict the shear capacities of the flexural members associated with the given flexural deformation conditions. To verify the proposed strut-and-tie model, the estimated shear strengths depending on the flexural deformation are compared with the experimental results. The experimental data are in good agreement with the values obtained by the proposed model.

Production of Ginsenoside Rd from Ginsenoside Rc by ${\alpha}-{\small{L}}$-Arabinofuranosidase from Caldicellulosiruptor saccharolyticus

  • Shin, Kyung-Chul;Lee, Gi-Woong;Oh, Deok-Kun
    • Journal of Microbiology and Biotechnology
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    • v.23 no.4
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    • pp.483-488
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    • 2013
  • Ginsenoside Rd was produced from ginsenoside Rc using a thermostable recombinant ${\alpha}-{\small{L}}$-arabinofuranosidase from Caldicellulosiruptor saccharolyticus. The optimal reaction conditions for the production of ginsenoside Rd from Rc were pH 5.5, $80^{\circ}C$, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% (v/v) n-hexane. Under these conditions, the enzyme produced 7.0 g/l ginsenoside Rd after 30 min, with a molar yield of 100% and a productivity of 14 g $l^{-1}\;h^{-1}$. The conversion yield and productivity of ginsenoside Rd are the highest reported thus far among enzymatic transformations.

Planar plastic flow of polymers near very rough walls

  • Lyamina, Elena A.;Date, Prashant P.
    • Structural Engineering and Mechanics
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    • v.58 no.4
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    • pp.707-718
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    • 2016
  • The main objective of the present paper is to investigate, by means of a boundary value problem permitting a semi-analytic solution, qualitative behaviour of solutions for two pressure-dependent yield criteria used for plastically incompressible polymers. The study mainly focuses on the regime of friction (sticking and sliding). It is shown that the existence of the solution satisfying the regime of sticking depends on other boundary conditions. In particular, there is such a class of boundary conditions depending on the yield criterion adopted that the regime of sliding is required for the existence of the solution independently of the friction law.

Optimization of Lactic Acid Production in SSF by Lactobacillus amylovorus NRRL B-4542 Using Taguchi Methodology

  • Nagarijun Pyde Acharya;Rao Ravella Sreenivas;Rajesham Swargam;Rao Linga Venkateswar
    • Journal of Microbiology
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    • v.43 no.1
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    • pp.38-43
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    • 2005
  • Lactic acid production parameter optimization using Lactobacillus amylovorus NRRL B-4542 was performed using the design of experiments (DOE) available in the form of an orthogonal array and a software for automatic design and analysis of the experiments, both based on Taguchi protocol. Optimal levels of physical parameters and key media components namely temperature, pH, inoculum size, moisture, yeast extract, $MgSO_4{\cdot}7H_20$, Tween 80, and corn steep liquor (CSL) were determined. Among the physical parameters, temperature contributed higher influence, and among media components, yeast extract, $MgSO_4{\cdot}7H_20$, and Tween 80 played important roles in the conversion of starch to lactic acid. The expected yield of lactic acid under these optimal conditions was 95.80% and the actual yield at optimum conditions was 93.50%.