• Title/Summary/Keyword: y-linolenic acid

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Determination of Saturated and Unsaturated Fatty Acids Amount in Leukocyte Membranes from Subjects Fed with Solid and Fluid Oils

  • Erman, Fazilet;Aydin, Suleyman;Demir, Yasar;Akcay, Fatih;Bakan, Ebubekir
    • BMB Reports
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    • v.39 no.5
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    • pp.516-521
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    • 2006
  • Modifications in dietary fatty acid intake might lead to a modification in membrane phospholipid fatty acid composition. The purpose of this study was to investigate relationship between different type of oil consumption and leukocyte membrane phospholipid composition. This study was carried out in subjects utilizing butter (n = 15), margarine (n = 15), fluid oil (n = 15) and mixed types of oils (n = 15) in total 60 subjects. Leukocytes were separated from total blood by dextran sedimentation method. Membrane lipids and proteins were isolated following the cell disruption. Fatty acids of membrane phospholipids were isolated by hydrolysation with phospholipase B under ultrasonic dismembranator. Free fatty acids were identified with gas chromatography at chloroform phase. The results obtained were compared with data obtained by chromatograms of the standards. Results more prominent values of arachidic, dihomo-$\gamma$-linolenic and palmitoleic acids were found in butter-or mixed oil-user groups; eicosadienoic, eicosamonoenoic, dihomo-$\gamma$-linolenic and behenic acids in fluid oil; heptanoic, valeric, eicosadienoic and linolenic acids in margarine groups. The fatty acid composition of mixed oil was similar to butter, while other two oils were so different. From this study, it was concluded that the type of oil consumption might have an influence on phospholipid components of plasma membranes.

Chemical Components of Acanthopanax divaricatus and Anticancer Effect in Leaves (개오가피의 성분 및 항암효과)

  • Yook, Chang-Soo;Rho, Young-Soo;Seo, Seong-Hoon;Leem, Jae-Yoon;Han, Dug-Ryong
    • YAKHAK HOEJI
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    • v.40 no.3
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    • pp.251-261
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    • 1996
  • Acanthopanax divaricatus Seem. is a medicinal plant growing widely through out Korea, Japan and China. The plant material of Acanthopanax spp. is used as analgesic , tonic, sedative drug as well as for the treatment of hypertension, rheumatism and diabetes. From the stem barks and root barks of A. divaricatus, diterpenoid compound was isolated and identified as pamaric acid ($C_{20}H_{30}O_2,\;mp\;164^{\circ}C$), lignan compounds were isolated and identified as d-sesamin ($C_{20}H_{18}O_6,\;mp\;123{\sim}124^{\circ}C$), eletheroside E ($C_{34}H_{46}O_{18},\;mp\;257{\sim}259^{\circ}C$), three sterol compounds were identified as ${\beta}$-sitosterol, campesterol, stigmasterol, and six fatty acid compounds were identified as stearic acid, palmitic acid, oleic acid, linolenic acid, arachidonic acid and behenic acid. And also leaves of A. divaricatus, chiisanoside were identified, one of secotriterpenoidal compound(white amorphorous powder crystal, mp $228^{\circ}C$). Anticancer activity and nephrotoxicity were tested by MTT assay. Anticancer effect of chiisanoside was much lower than that of cisplatin.

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Total Lipid Content and Fatty Acid Composition in Setaria italica, Panicum miliaceum and Sorghum bicolor (조, 기장, 수수의 지방질과 지방산 조성)

  • 하영득;소한섭;이삼빈
    • Journal of the East Asian Society of Dietary Life
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    • v.12 no.2
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    • pp.123-128
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    • 2002
  • Fatty acid compositions of Setaria italica, Panirum miliaceum and Sorghum bicolor were determined with total lipids extracted. Total lipid content of Setaria italica, Panium miliaceum and Sorghum bicolor were 3.9%(w/w), 2.7%(w/w) and 2.3%(w/w), respectively. Total lipids were fractionated into neutral lipids, glycolipids and phospholipids by the silicic acid column chromatography. Neutral lipids were the most abundant component. Among fatty acids separated by GC, linoleic acid was determined as a major fraction in Setaria italica, Panicum miliaceum and Sorghum bicolor. Palmitic acid and oleic acid were also separated as second major components. In Setaria italica, behenic acid was separated from the phospholipid fraction and myristic acid from the neutral lipid fraction. Linolenic acid was not detected in all the samples.

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Effects of Dietary Fatty Acids on Serum Lipids and Fatty Acid Composition of Serum Phospholipids in Men

  • Joo, Oh-Eun;Sook, Kwon-Jong;Kyung, Chang-Yu
    • Journal of Nutrition and Health
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    • v.30 no.4
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    • pp.415-424
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    • 1997
  • Serum lipid levels and fatty acid composition of serum phospholipids(PL) were investigated in 24 healthy male subjects who consumed either corn oil(CO) rich In linoleic acid(LA), perilla oil(PO) rich in $\alpha$-linolenic acid($\alpha$-LNA), or canola oil acid(OA) as a major fat source fir 5 weeks. The PO and the CNO groups showed significant(P <0.05) increases in serum high density lipoprotein-cholesterol(HDL-C) levels and in ratios of HDL- C/total cholesterol(TC) compared with initial values measured at the beginning of the study. Significantly(p <0.05) increased concentrations of serum triglycerides(TG) ware observed after 5 weeks of the CO based diet compared with both its intial value and the concentration observed after 5 weeks with the PO-based diet. fatty acid composition of serum PL reflected changes in dietary fatty acid composition and metabolism. Compared with the initial levels, significandy increased contents of eicosapentaenoic acid(EPA) and docosahexaenoic acid(DHA) were observed in serum PL of the PO group and significantly increased contents of $\alpha$-LNA and EPA were observed in the CNO group. Arachidonic acid(AA) content of serum PL did not change in the CO group during the study period, although, the increase in LA was significant(P <0.05). Compared with the CO-based diet, both the PO and the CNO-based diets seem to have beneficial effects on atherosclerosis by influencing the serum lipid profile and fatty acid composition of serum PL.

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Analysis of Chemical Composition in leaf and foot of Acrorus calamus L. (창포(Acorus calamus L. var. angustatus Bess.) 잎과 뿌리 중의 성분 분석)

  • Kim, Hye-Ja;Kim, Se-Won;Shin, Chang-Sik
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.37-41
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    • 2000
  • This study has been carried out to investigate the chemical composition of Acorus calamus L., a wild plant (Chang Po) which has long been used as a medicine or a hair rinse in Korea and China. Potassium was found to be the most predominant mineral in leaf and root, followed by calcium, magnesium, and phosphorus, in a decreasing order. Contents of potassium in leaf and root account for 54a.65 mg% and 242.87 mg%, respectively. Contents of these minerals in leaf were higher value than that in root. Glucose and fructose were found to be the major free sugars in leaf and root. Malic acid was the most abundant organic acid of leaf and root, and followed by citric acid. Fourteen different kinds of fatty acids were identified from the leaf and root. Content of unsaturated fatty acids. such as linolenic, and linoleic acid ware higher than that of saturated fatty acids. Content of total amino acids was 3 times higher in leaf than that in root. Leucine was the major amino acid in leaf and glutamic acid in root.

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Studies on the development of cocoon pupas for food materials (번데기의 식품용도개발(食品用途開發)에 관(關)한 연구(硏究))

  • Yu, Tae-Jong;Lee, Kwang-Yul;Lee, Sang-Keun
    • Journal of Nutrition and Health
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    • v.11 no.1
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    • pp.39-43
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    • 1978
  • These experiments were made to investigate the characteristics of cocoon pupas. The proximate composition and fatty acids were analyzed. In order to eliminate the unfavorable odor of cocoon pupas, alkaline treatment and extraction of fat were conducted. The results were as follows. 1) The unfavorable odor can be eliminated through the extraction of fat. Boiling with n-hexane for 1 hour was the best. 2) The cocoon pupas were contaminated with $10^8$ bacterial counts/g at the first eating state. When they were stored at room temperature for 6 days, bacterial counts did not increase inure than $10^8$, but they were putrefacted with bad odor. 3) The powder of defatted cocoon pupas prepared for food material contained around 1.3% moisture, 76.0% protein, 0.8% crude fat, and 4.8% ash. 4) It was not efficient to eliminate unfavorable odor by alkaline treatment. 5) The fatty acids of cocoon pupas are composed of 0.35% myristic acid, 20.90% palmitic acid, 0.5% palmitoleic acid, 7.11% stearic acid, 32.20% oleic acid, 5.48% linoleic acid and 30.31% linolenic acid.

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The Nutritional Components of Aerial Whole Plant and Juice of Angelica keiskei Koidz (명일엽 전초 및 생즙의 영양성분 분석)

  • Kim, Ok-Kyung;Kung, Sung-Sil;Park, Won-Bong;Lee, Myung-Whan;Ham, Seung-Shi
    • Korean Journal of Food Science and Technology
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    • v.24 no.6
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    • pp.592-596
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    • 1992
  • This study was undertaken to analyze proximate compositions, minerals, vitamins, amino acids and fatty acids of Angelica keiskei Koidz. The contents of raw leaf and stem juice were compared with those of aerial whole plant. The aerial whole plant was relatively rich in crude fiber. The leaf juice was rich in lipid, protein, minerals, vitamins and amino acids whereas the stem juice was rich only in sugar and some kinds of fatty acids.

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Biological activities of an entomogenous fungus, Paecilomyces tenuipes grown on silk worm (눈꽃동충하초의 생리활성에 관한 연구)

  • Hur, Hyeon;Lee, Min-Woong;Hong, In-Pyo
    • Journal of Mushroom
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    • v.3 no.2
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    • pp.90-94
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    • 2005
  • This study was carried out to investigate the biological activities of P. tenuipes formed on silkworm (Bombyx mori) variety. The mean content of total amino acid in the fruiting bodies of P. tenuipes was $1.03{\mu}mole/g$. The distribution rate of amino acid components decreased in the order of Arginine(12.2%) > Glycine(10.5%) > Proline(9.6) > Tyrosine(8.9%) > Serine > Leucine > Threonine. The most abundant amino acid in the fruiting bodies of the Baegokjam, Chilbojam and Hachojam infected with P. tenuipes was arginine, while Yangwonjam was Glycine. The most abundant fatty acid in P. tenuipes was Oleic acid on a dry weight basis. The unsaturated fatty acids such as Oleic acid, Linoleic acid and Linolenic acid accounted for more than 78% of the total fatty acids.

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Comparison of Fatty Acid and Nutritional Composition of Korean Native Black Cattle and Hanwoo (한국 재래흑우 및 한우의 지방산 조성 및 영양성분 비교)

  • Park, Kyung-Sook;Park, Hyun-Suk;Choi, Young-Joon;Lee, Jae-Sang;Park, Sung-Suk;Jung, In-Chul
    • Korean journal of food and cookery science
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    • v.30 no.5
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    • pp.556-563
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    • 2014
  • The nutritional composition of Korean native black cattle (KNBC) and Hanwoo beef were compared. According to the results, there were no significant differences in moisture, protein, ash, calorie and collagen content, but the fat content of KNBC was significantly higher than that of Hanwoo (p<0.05). The P, Na and Cu content of KNBC were significantly higher than that of Hanwoo (p<0.05). The KNBC and Hanwoo had total mineral amounts of 4,052.34 and 3,214.44 ppm, respectively, with the KNBC being significantly higher than Hanwoo (p<0.05). The vitamin B1 content of KNBC was significantly higher than that of Hanwoo (p<0.05), but there was no significant difference in B2 content between the samples. The total structural amino acid contents was not significantly different between KNBC (12.35%) and Hanwoo (12.58%). The KNBC and Hanwoo had total free amino acid amounts of 752.698 and 661.795 ppm, respectively, with the KNBC being significantly higher than Hanwoo (p<0.05). The stearic acid (10.631%), linoleic acid (2.271%), linolenic acid (0.065%) and tricosanoic acid (0.038%) contents of KNBC were lower than those of Hanwoo (p<0.05). However, their palmitoleic acid (5.292%), oleic acid (48.815%), ${\gamma}-linolenic$ acid (0.094%), eicosenoic acid (0.042%) and docosadienoic acid (0.099%) contents were higher than those of Hanwoo (p<0.05). There was no significant difference between the KNBC and Hanwoo in the saturated fatty acid : unsaturated fatty acid ratio.

Breeding for Improvement of Fatty Acid Composition in Rapeseed Brassica napus L. XVI. Effect of Fertilizer level on the Oil Content and Fatty Acid Composition of Rapeseed (유채 지방산조성 개량육종에 관한 연구 - 제 16 보 유채 시비수준이 유지함량 및 지방산조성에 미치는 영향-)

  • Kwon, B.S.;Lee,J.I.;Kim, S.K.;Chee, Y.A.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.2
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    • pp.198-202
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    • 1984
  • This experiment was conducted to investigate the oil content, change of fatty acid composition affected by ferlilizer levels in upland and paddy field cultivation of rapeseed, The oil content with the fatty acid such as palmitic, linoleic and linolenic was increased in the winter crop on drained paddy field compared with those of upland field in which oleic and stearic fatty acid was increased. Unsaturated, good quality fatty acid content such as oleic and linoleic acid in the cultivation of upland field was higher by 2-5% than those of paddy field. Oleic and linoleic fatty acid contents showed increased with increment of nitrogen fertilizer up to 15kg/10a, and showed same trend until 80kg/ha fertilization level of phosphate and potassium in upland field but there was no effect in paddy field cultivation.

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