• Horticultural Science & Technology
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• v.18 no.6
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• pp.808-810
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• 2000

The chemical components of xylem sap collected from kiwifruit (Actinidia chinensis Planch cv. Hayward) trees were analyzed and processing of xylem sap drink were accomplished to increase utilization of xylem sap as a drink. Water content, soluble solids, viscosity, and pH of the xylem sap were 99.60%, 0.90%, 1.01, and 6.50, respectively. In the xylem sap, fructose was the highest among free sugars followed by glucose, sucrose, galactose, and mannitol. The major inorganic components in the sap were calcium, potassium, and magnesium, and calcium was the dominant among them. Ten kinds of amino acids in the xylem sap were detected by amino acid analyzer, and the major amino acids were glutamic acid, lysine, and isoleucine. Glutamic acid was the most dominant amino acid in the xylem sap. Major compositions of xylem sap drink were 74.5% xylem sap, 15% kiwifruit puree and 10% high fructose. Nutritional facts in drink (252 mL) processed by using the xylem sap were 21.8 g sugar, 23.1 mg calcium, 14.1 mg potassium, 554.5 mg amino acid, and 15.6 mg ascorbic acid.

• Journal of Life Science
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• v.8 no.1
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• pp.97-101
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• 1998

Experiments were conducted to investigate the bleeding xylem sap characteristics, mineral contents and endogenous plant hormon contents in cucumbeer and origental melen plant grafted onto different rootstocks and various cucubitaceous crops developed for rootstocks. The volume of xylem sap, expressed as milliliter per 100g plant flesh weight, was high in the Heukzong rootstock plant as compared to the other rootstock plant,. Acidity(pH) and electric conductivity(EC) values in breeding xylem sap were not much influenced by the kind of rootstocks. Incucumber plant grafted onto different rootstocks, the contents of xylem sap in total nitrogen, phosphorus and K were increased by grafting, whereas those of Ca and Mg were decreased. Rootstocks had no influence on the total nitrogen contents in xylen sap of ‘Sineuncheon’, but decreased K Contents. Conecntration of cytokinin-like substances in origental melon xylem sap was a little higher in Sintozwa and ungrafted.

• Horticultural Science & Technology
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• v.17 no.6
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• pp.742-746
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• 1999

Contents of mineral element and cytokinin in the xylem sap of 'Keumdongee' and 'Tongilhwang' oriental melons were compared with those in oriental melons grafted onto 8 rootstocks. The effect of grafting on the fruit quality of oriental melon was also investigated. Flesh firmness varied with rootstocks. Soluble solids contents in the placenta tissue of grafted 'Tongilhwang' were higher than that in the 'Keumdongee'. Electric conductivity of the xylem sap in own-rooted plants was higher in 'Keumdongee' than in 'Tongilhwang', but it increased in 'Tongilhwang' once they were grafted. The sap volume per plant was greater in 'Keumdongee' than in 'Tongilhwang'. The mineral concentrations varied considerably depending on the rootstock used. Xylem sap of grafted oriental melons contained a higher amount of mineral ions, especially $NO_3{^-}$ and $PO_4{^-}$, than did the sap in own-rooted plants. The increase in the mineral levels in sap due to grafting was most apparent in 'Tongilhwang'. Xylem sap from both 'Keumdongee' and 'Tongilhwang' contained trans-zeatin (t-Z), trans-zeatin riboside (t-ZR), and dihydrozeatin riboside (DHZR). Small amounts of isopentenyl adenine (IPA) and isopentenyl adenine riboside (IPAR) were also detected. Trans-zeatin riboside was the most abundant, followed by t-Z. Cytokinin concentration in 'Keumdongee' was not significantly influenced by rootstock type used, although the highest concentration of cytokinins in 'Keumdongee' was obtained with 'Chamtozwa' rootstock. However, the cytokinin concentration in 'Tongilhwang' increased with grafting irrespective of rootstock type used.

• Food Science and Preservation
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• v.14 no.3
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• pp.294-300
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• 2007

Three varieties of soy sauce were prepared using saps of bamboo, xylem and gorosoe, with sea salt and bamboo salt, in May. Soy sauce was fermented in the traditional manner. Homemade soy sauce was analyzed after 3 months of aging. The pH and amino-type nitrogen content of soy sauce fermented with xylem sap and sea salt were very high, and malic acid was dominant among four types of organic acid. The content of calcium in soy sauce fermented with bamboo sap and sea salt was fourfold greater than that of the control. The contents of total amino acids in the soy sauces fermented with xylem sap and gorosoe, with the addition of sea salt instead of water, were 2-fold and 1.4-fold greater than that of ordinary soy sauce, respectively. In the DPPH assay, the antioxidant effect of soy sauce fermented with bamboo sap and sea salt was higher than that of other samples. Sensory evaluation showed that the overall acceptability of soy sauce fermented with gorosoe and sea salt was best, among the samples tested.

• Korean Journal of Environmental Agriculture
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• v.25 no.2
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• pp.164-169
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• 2006

This study was conducted for 10 days from 17 July to 26 July in 2005 to measure the amount of xylem sap flow under short-term variation of soil moisture regimes at -20 kPa, -50 kPa and -80 kPa in eight-year-old 'Fuji'/M.9 apple trees with different fruit loads. Fruit load was adjusted as three different treatments with standard (100%), 1/2 times (50%) and 2 times (200%) on the basis of optimum fruiting number per tree as the standard fruit load of Fuji cultivar. Trees with standard fruit load during the experimental period showed higher xylem sap flow at -50 kPa of soil moisture regimes than those of trees with 1/2 times and 2 times fruit load. Trees with 1/2 times and 2 times fruit load had similar patterns of the diurnal changes of xylem sap flow, vapor pressure deficit (VPD), and maximum evapotranspiration (ETm). However, trees with 2 times fruit load at -50 kPa and -80 kPa of soil moisture regimes produced lower amount of xylem sap flow than ETm. Trees with standard fruit load produced $1.06{\sim}3.93$ L/tree more amount of xylem sap flow than ETm at all soil moisture regimes. But xylem sap flow of tees with 2 times fruit load had 21% lower at -50 kPa and $31{\sim}36%$ lower at -20 kPa and -80 kPa of soil moisture regimes, respectively than that of trees with standard fruit load. Shoot growth and leaf area were significantly the highest in trees with standard fruit load while those of trees with 2 times fruit load recorded significantly lowest. Leaf water potential of trees with standard fruit load was lower than that of trees with 1/2 times and 2 times fruit load. It indicated that tees with standard fruit load had higher water use for transpiration than other treatments and tees with 2 times fruit load received more stress for the transpiration process under low soil moisture regimes. Consequently, 'Fuji'/M.9 apple trees, the fruit load and soil moisture should be maintained optimum to increase xylem sap flow and transpiration during higher growth period.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.910-919
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• 2012

The role of inorganic nitrogen assimilation in the production of amino acids, organic acids and soluble sugars is one of the most important biochemical processes in plants, and, in order to achieve normally, nitrate uptake and assimilation is essential. For this reason, the characterization of nitrate assimilation and metabolite composition from leaves, roots and xylem sap of tomato (Solanum lycopersicum) was investigated under different nitrate levels in media. Tomato plants were grown hydroponically in liquid culture under five different nitrate regimes: deficient (0.25 and 0.75 mM $NO_3{^-}$), normal (2.5 mM $NO_3{^-}$) and excessive (5.0 and 10.0 mM $NO_3{^-}$). All samples, leaves, roots and xylem sap, were collected after 7 and 14 days after treatment. The levels of amino acids, soluble sugars and organic acids were significantly decreased by N-deficiency whereas, interestingly, they remained higher in xylem sap as compared with N-normal and -surplus. The N-excessive condition did not exert any significant changes in metabolites composition, and thus their levels were similar with N-normal. The gene expression and enzyme activity of nitrate reductase (NR), nitrite reductase (NIR) and glutamine synthetase (GS) were greatly influenced by nitrate. The data presented here suggest that metabolites, as a signal messenger, existed in xylem sap seem to play a crucial role to acquire nitrate, and, in addition, an increase in ${\alpha}$-ketoglutarate pathway-derived amino acids under N-deficiency may help to better understand plant C/N metabolism.

• Journal of Plant Biology
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• v.36 no.4
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• pp.363-370
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• 1993

Experiments with non-nodulated alfalfa (Medicago sativa L. cv, Europe) plants grown in hydroponic cuiture, were carried out to estimate the remobiJization of nitrogen (N) reserves and to investigate the transported forms of reduced soluble-N in xylem during regrowth following shoot removal. Endogenous N remobilization were estimated by $^{15}N$ labelling and amino acids in xylem sap were analysed. The, $^{15}N$ contents of ~egr9wing leaves and stems increased as a result of remobilization of N reserves mainly from root ,system, Regrowing leaves were a stronger sink than regrowing stems, with about tWo-thirds of remobilized 15N being recoved in leaves. Endogenous N in lateral roots accounted for about 46% of the total N reserves used for regrowth, while tap roots accounted for 23%, About 72% of total endogenous N remobilized to regrowing shoot, occurred during the first 10 days of regrowth, The outflow of reduced soluble-N (mainly amino acids) was greater than that of protein-N, while the latter was the largest storage pool in tap root and lateral roots. It is suggested that amino acids-N was the most readily avaiable form of N reserves. Asparagine, which repre5ented about 75% of amino acids-N in xylem sap, was the main transported form of reduced N. Its relative contents, during the first 10 days of regrowth, decreased from 75% to 59%. This decline was accompanied by compensatory increase in the relative contents of asparatate and glutamine.

• 한국가시화정보학회:학술대회논문집
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• 2003.11a
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• pp.83-86
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• 2003

Synchrotron X-ray micro imaging technique was employed to non-invasively monitor the water flow inside xylem vessels in a bamboo leaf. The phase contrast X-ray images clearly visualized plant anatomy and the rise of a water front inside the vessels. Consecutive X-ray images taken for 60 seconds revealed water rise kinetics against gravity in the xylem of a cut dry leaf taken from a bamboo tree. For the first time, traces of water rise, variation of contact angle between water and xylem wall as well as the internal structure of xylem were obtained. In xylem vessels, a repeating flow pattern has a typical flow velocity of $30.7{\mu}m/s$ and faster flow is established intermittently. It is concluded that the transmission type of X-ray micro imaging can be used as a powerful tool to investigate the ascent of sap in the xylem vessels at a resolution higher than that of MRI.

• Journal of the Korean Society of Visualization
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• v.5 no.1
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• pp.30-36
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• 2007

The global environment is deteriorating at an alarming rate, despite of enhanced international environmental regulation. Many studies have been performed to reduce toxic pollutants. Recently, plant-based phytoremediation technology for moving toxic contaminants from soil and water has been receiving large attention. Arsenic-contaminated soil is one of the major pollutant sources for drinking water. Pteris erotica has been known as a hyper-accumulator of arsenic from soils. In this study, we investigated the effect of arsenic absorption on sap flow inside xylem vessels of Pteris. The synchrotron X-ray micro-imaging technique was employed to monitor the refilling process of water containing arsenic inside the xylem vessels of Pteris's leaves and stems non-invasively. The captured phase-contrast X-ray images show both anatomy of internal structure and transport of water inside Pteris. The exposure of Pteris to arsenic solution was found to increase largely the water raise speed in xylem vessels. The present results would provide important information needed for understanding the mechanisms of accumulation and transportation of toxic materials in plants.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.11
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• pp.1612-1617
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• 2003

Synchrotron X-ray micro imaging technique was employed to non-invasively monitor the water flow inside xylem vessels in a bamboo leaf. The phase contrast X-ray images clearly visualized plant anatomy and the rise of a water front inside the vessels. Consecutive X-ray images taken for 60 seconds revealed water rise kinetics against gravity in the xylem of a cut dry leaf taken from a bamboo tree. For the first time, traces of water rise, variation of contact angle between water and xylem wall as well as the internal structure of xylem were obtained. In xylem vessels, a repeating flow pattern has a typical flow velocity of 30.7$\mu\textrm{m}$/s and faster flow is established intermittently. It is concluded that the transmission type of X-ray micro imaging can be used as a powerful tool to investigate the ascent of sap in the xylem vessels at a resolution higher than that of MRI.