• 대한족부족관절학회지
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• 제21권3호
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• pp.113-116
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• 2017

Synovial fistula of the ankle joint is an uncommon complication from an ankle sprain. This may likely result in an unhealed wound around the ankle joint due to continuous leakage of joint fluid. However, in the event of an open wound on the lower leg, and not on the joint, it may be difficult to consider synovial fistula as a cause of the open wound. We experienced an interesting case with an unhealed open wound on the lower leg due to a synovial fistula of the ankle joint following an ankle sprain. We obtained good results after a treatment using a self-produced suction drainage device on the unhealed open wound.

• Journal of Applied Biological Chemistry
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• 제61권4호
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• pp.351-355
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• 2018

Treatment towards wound healing, a complex and dynamic process, has been given a great deal of efforts in the last few decades. Focus has been imposed on developing wound dressings that meet the requirements for proper wound healing. In this study, hydrogel made from blends of poly (vinyl alcohol) and ${\beta}$-1,6-branched-${\beta}$-1,3-glucan (beta-glucan) were synthesized by modified solvent casting method for wound dressing application. Optimization of hydrogel composition and analysis of wound dressing parameters such as stability and fluid uptake capacity (in the presence of water, saline and different pH solutions) has been studied. The result indicated that the PVA/beta-glucan hydrogel hold its structural integrity even at alkaline pH (pH~9) and upholds fluids four times of its original weight. Thus, the developed hydrogel is expected to be a promising candidate as wound dressing.

• 대한수의학회지
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• 제46권4호
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• pp.305-313
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• 2006

The primary goal of the wound healing is rapid wound closure. Recent advances in cellular and molecular biology have greatly expanded our understanding of the biologic processes involved in wound repair and tissue regeneration. This study was conducted to develop a new sponge type of biomaterial to be used for either wound dressing or scaffold for tissue engineering. We designed to make a comparative study of the wound healing effect of silk fibroin/hyaluronic acid (SF/HA) blend sponge in full-thickness dermal injury model of rat. Two full-thickness excisions were made on the back of the experimental animals. The excised wound was covered with either the silk fibroin (SF), hyaluronic acid (HA) or SF/HA (7 : 3 or 5 : 5 ratio) blend sponge. On the postoperative days of 3, 7, 10 and 14, the wound area was calculated by image analysis software. Simultaneously, the tissues were stained with Hematoxylin-Eosin and Masson's trichrome methods to measure the area of regenerated epithelium and collagen deposition. In addition, we evaluated the degree of the epithelial cell proliferation using immunohistochemistry for proliferating cell nuclear antigen (PCNA). We found that the half healing time ($HT_{50}$) of SF/HA blend sponge treated groups were significantly decreased as compared with either those of SF or HA treatment group. Furthermore, SF/HA blend sponges significantly increased the size of epithelialization and collagen deposition as well as the number of PCNA positive cells on epidermal basement membrane as compared with those of control treatment. Especially, the 5 : 5 ratio group of SF/HA among all treatment groups was most effective on wound healing rate and histological studies. These results suggest that SF/HA blend sponges could accelerate the wound healing process through the increase of epithelialization, collagen deposition and basal cell proliferation in full thickness skin injury.

• 동의생리병리학회지
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• 제31권6호
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• pp.334-340
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• 2017

Wound healing is the restoration in injured skin tissue and one of the most important therapeutic targets. The process consists of inflammation, proliferation, and remodeling. There have been reported multiple factors that accelerate and delay wound healing. In this study we tested the hypothesis that Sophorae Radix extract would improve the recovery of wound healing after full-thickness skin wound in rats. Twenty young male Sprague-Dawley rats were used for the studies. A full-thickness skin wound was made on the dorsal skin of the rats. Either Sophorae Radix water extract (SR) or saline (Control) was orally administrated every day. The wound area was measured and the percentages of wound contraction, wound healed and wound epithelization were evaluated. After 15 days, the skin tissues were excised and examined by histopathological and immunohistochemistrical method. In results, wound area in SR group was significantly decreased to compared with Control. SR group showed the significant enhancements in the percentages of wound contraction, wound healed and wound epithelization. Histopathological examination revealed that SR induces neo-vascularization potential in wound healing process. SR treatment in rats significantly accelerated cutaneous wound healing in the angiogenesis process by increasing VEGF and TGF-${\beta}1$ synthesis. These results suggest that Sophorae Radix enhance skin wound repair by increasing the angiogenic agents such as VEGF and TGF-${\beta}1$.

• The Journal of Korean Physical Therapy
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• 제9권1호
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• pp.1-18
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• 1997

The purpose of this study is to investigate the effects of the low power Helium Neon-Infra Red(HeNe-IR) laser and the special electromagnetic therapeutic apparatus stimulation, which is usually designated as TDP by using the initial of Tending Diancibo Pu which is the Chinese inscribed with English, on wound healing in rat. The seventy-five Sprague-Dawley adult female and male rats were assigned to the experimental and control groups. Each rat was anesthetized with pentobarbital sodium, and three full-thickness incisions with 12mm length wert made on the back of the half cf the rats and three deep second degree burns were made on the back of the remainder of the rats. From 34 hours after being injured, the rats of the experimental laser group were irradiated with the 157mW electric power HeNe-IR laser for 2 minutes every day and the rats of the experimental TDP group were stimulated with TDP irradiation with the 4km spot distance for 20 minutes every day during the 17 days. The rats were sacrificed and the wound parts of its were incised on the first day, 4th day, 7th day, 10th day and 17th day respectively after the beginning of wound treatment with laser and TDP irradiation. The incised wound parts were processed appropriately for the light microscopic and electron microscopic examination. The length of incised wound was measured with microcaliper before the wound part was incised. There was a significant decrease in the length of the incised wound of the experimental laser and TDP group, compared with that of control group at 4th day, 7th day and 10th day(p<0.01) after surgery. Through the histological examination of the wound site, the more rapid epithelialization and collagen formation in experimental groups were showed, compared with control group. The histological results were analyzed and summarized as the follows; The epidermis begins to be regenerated and the granulation tissue begins to be changed to the mature pattern in the H-E stained incised skin of the laser and TDP treatment group on the 4th day. The epidermis shows the complete regeneration and the granulation tissue in the dermis in mostly to be matured in the laser and TDP treatment group on the 7th day, compared with control group. The chronic inflammatory cells are oberved and the necrosis of the collagen fibers are partially observed in control group on the 10th day. The dermis of the laser and TOP treatment group reveals relatively compactly arranged collgen bundles with the mature collagen fibers on the 10th day. The epidermis and dennis of the laser and TDP group are repaired normally and the hair follicles are well regenerated on the 17th day. The mild edema and the granulation tissue is observed in the dermis of the control groups and the delayed treatment process is observed on the 17th day. The Most of proliferated collagen fibrils are found to be compact and regular in electron micrograph of burn skin of the laser treatment group on the 10th day hut the interstitial eadema and some inflammatory cells are found in the control group. The above results suggest that through the visual and histological examination the epithelized epithelium and the proliferation of the collagen liters in the dermis occur very effectively with the low power laser treatment and the TDP treatment in the incised wound healing and the burn wound healing.

• Archives of Plastic Surgery
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• 제36권5호
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• pp.548-554
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• 2009

Purpose: To evaluate clinical efficacy and safety of hyaluronic acid based autologous dermal fibroblasts (Hyalograft 3D) in the treatment of diabetic foot ulcers. Methods: A total of 28 patients with diabetic ulcers were randomized to either the control group with nonadherent foam dressings(n=14) or the treatment group with autologous tissue - engineered grafts(n=14). Weekly assessment contained vital sign checks, ulcer size measurements, and wound photos. At 12th week, percentages of complete wound healing and mean healing times were compared. Safety was also monitored by adverse events. Results: Complete wound healing was achieved in 84.6% of the treatment group and 23.1% of the control group (p<0.005). The mean times of closures for the treatment versus control groups were 6.1 weeks and 10.9 weeks, respectively. No adverse events related to the study treatment occurred. Conclusion: The use of hyaluronic acid based autologous fibroblast grafts was found to be a safe and effective treatment for diabetic foot ulcers.

• 한국임상수의학회지
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• 제11권1호
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• pp.427-436
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• 1994

To investigate the effect of yeast glucan on wound healing in rabbits, histopathological findings and wound contraction after topical application of glucan on the open wound were observed. Formalin fixation and embedding of tissues at wound were prepared for histopathological findings at the other day after wounding. Wound size was measured for contraction. Macrophages appeared in the deeper site under the scab at day 1 after wounding in the glucan treated group, but not in the control. A number of macrophages, fibroblasts and a small number of neutrophils were observed at day 3 after wounding In the glucan treated group as compared with the control. Histopathological findings at day 5 after wounding in control were similar with those at day 3 in the glucan treated group. Glucan like granules were scattered in the cytoplasm of macrophages at day 5 in the glucan treated group. A great portion of wound reepithelialized at day 5 in the glucan treated group but a small portion of wound in the control. The Present study indicated that the treatment of 5,000$\mu\textrm{g}$/$m\ell$ of glucan has an effect on wound heal. ins in respect to migration of macrophage into the wound site, granulation tissue formation and epithelialization.

• Journal of Animal Science and Technology
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• 제64권5호
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• pp.997-1007
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• 2022

Delayed healing associated with distal limb wounds is highly challenging in equine clinical practice. This study aimed to evaluate healing rates between chronic non-granulating wounds of horse distal limbs that were treated with maltodextrin/ascorbic acid gel alone or in combination with povidone-iodine 1% solution and those treated with povidone-iodine 1% only throughout the study period (35 days) in clinical settings. The study was conducted on 18 adult horses (3-15 years old). Based on the treatment regimen utilized, the horses were divided into three groups (n = 6), with each group having a similar mean wound area. The percentages of wound contraction, epithelialization, and overall wound healing were determined weekly for each wound. By the end of the study, the total wound healing percentage was significantly increased between the study groups (p < 0.05). The use of maltodextrin/ascorbic acid gel resulted in considerable wound contraction, rapid epithelialization, and complication-free wound healing. Based on the findings of this study, maltodextrin/ascorbic acid gel, independently or in combination with a 1% povidone-iodine solution, might be applied as a safe and effective wound healing promoting agent in horses with chronic non-granulating wounds.

• 농업과학연구
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• 제48권3호
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• pp.567-574
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• 2021

In this study, we evaluated the wound healing rate and, inflammatory cells effects of by Abeliophyllum distichum Nakai (ADN) extract in mice. We also assessed the stability of the ADN extract upon exposure to sunlight. Treatments were as follows: 1) CON (only saline solution), T1 (CON + 0.0125% ADN extract), T2 (CON + 0.05% ADN extract), and T3 (CON + 0.5% ADN extract). A 4 mm punch was used in the central part of the dorsal area to separate it from the subcutaneous tissue, causing a full-thickness skin wound. An amount of 1 mL of each sample was sprayed onto the treatment section of the wound with a pipette every day from the day of wound creation, with proper application ensured using brush. In the stability test, the pH was measured at 1, 4, and 8 weeks after exposing the samples of each treatment section to sunlight considering, the higher concentrations of the ADN extract. The results of this study indicate that the effectiveness of the wound contraction rate in the mice to which the ADN extract was applied was low. Moreover, the stability of the sample containing a high concentration of the ADN extract could not be verified. In addition, no significant results were obtained in the inflammatory reaction assessment. Therefore, additional research focusing on wound contraction, stability, and inflammatory cell outcomes of the ADN extract is needed.

• Journal of Ginseng Research
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• 제43권2호
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• pp.226-235
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• 2019

Background: Panax ginseng is regarded as one of the best compounds for promoting health, and it has been used traditionally as a medicinal herb. Recently, Korean Red Ginseng (RG) has been shown to protect skin from aging and wrinkling; it can also relieve atopic dermatitis and allergy symptoms. This study aimed to evaluate RG's effects on the regeneration of the full-thickness skin wounds in rat. Methods: Full-thickness skin wounds were generated in rats, and then RG was administered either orally or topically. The wound-healing effects of RG were investigated by assessing wound size, mRNA expression patterns of genes related to wound healing, histological staining, and measurements of lipid, moisture, and elasticity in skin tissues. Results: The wound size was smaller, and tissue regeneration rate was faster in the RG-treated group than that in the control group on days 15 and 20 after initiating treatment. On postoperative day 20, skin lipid and moisture content had increased significantly in the RG-treated group. Significant increases in the gene expression levels of transforming growth $factor-{\beta}1$ and vascular endothelial growth factor were found in the RG group during the early stages of wound healing. Matrix metalloproteinase-1 and matrix metalloproteinase-9 showed significant increases in gene expression levels on day 20. Conclusion: The results suggested that RG may promote healing of full-thickness skin wounds in rats. They also provided basic insights into the effects of RG on skin regeneration, supporting its use as a dressing material for wound treatment and its development as a functional food.