• Title/Summary/Keyword: work condition

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Optimization of gibberellin production by Fusarium prolifertum KGL0401 and its involvement in waito-c rice growth (Fusarium prolifertum KGL0401의 지베렐린 생산 최적조건과 waito-c 생장에 미치는 영향)

  • Rim, Soon-Ok;Lee, Jin-Hyung;Lee, In-Jung;Rhee, In-Koo;Kim, Jong-Guk
    • Journal of Life Science
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    • v.17 no.1 s.81
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    • pp.120-124
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    • 2007
  • Fusarium proliferatum KGL0401 was previously isolated from Physalis alkekengi var. francheti plant roots and exhibited higher GA productivity than wild type Gibberella fujikuroi. The :tim of this work was to find out an optimal culture condition for GA production. Various carbon(fructose, glucose, lactose, maltose, sucrose) and nitrogen($KNO_3$, urea, glycine, $NaNO_3,\;NH_4Cl$) sources were used for this study. GAs activities were analysed by gas chromatography and mass spectrometry(GC-MS). The highest yield of $GA_3$ was found in the growth medium supplemented with sucrose as carbon source and $NH_4Cl$ as nitrogen source. The optimum carbon-nitrogen concentration for $GA_3$ production was found to be 0.5 M:0.17 M. Supernatant was prepared from the culture fluid of F. proliferatum KGL0401 cultured for 7 days at 3 0'E and the 10 ul supernatant was treated with 2 leaf-rice seedling.

Ethylene Biosynthesis of an Alkalophilic Bacillus sp. Alk-7 (알카리성 Bacillus sp. Alk-7에 의한 Ethylene 생합성과 그 경로)

  • Bae, Moo;Kim, Mi-Ye
    • Microbiology and Biotechnology Letters
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    • v.26 no.3
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    • pp.195-199
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    • 1998
  • AH alkalophilic Bacillus SP. AIk-7, isolated from soil, produced ethylene. The characteristics of this microorganism is the ability to grow well under the alkaline condition, at pH 10.3. This strain is similar to Bacillus alkalophilus in terms of morphological, physiological and biological characteristics. In observation of relationship of cell growth and ethylene production according to incubation times, the ethylene synthesis mostly occur from the late exponential phase to the death phase of growth. The purpose of this paper is to study the effects of various substrates on the biosynthesis of ethylene in the intact cell and the cell-free system by the Bacillus sp. AIk-7. In both intact cell and cell-free extract, optimum conditions for ethylene production was achieved at pH 10.3 and 3$0^{\circ}C$. Ethylene was effectively produced from L-Met and 1-aminocyclopropane-1-carboxylic acid (ACC). In this case, ACC as the substrate on ethylene production were two fold higher than L-met at each concentration of substrates. On the other hand, the cell-free ethylene-forming system was used as a tool for the elucidation of the biochemical reaction involved in the formation of ethylene by Bacillus sp. AIk-7. Ethylene production in the cell-free system required the presence of manganese and cobalt ion to be stimulated a little. The result obtained in this work suggests that L-met and ACC may be a precursor more directly related to bacterial ethylene production than any other substrates tested.

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Studies on Stability for the Quality of Ginseng Products -4. Determination of Sorption Properties and Optimum Moisture Contents in Red Ginseng, Red Ginseng Powder and Red Ginseng Powder Tablet- (인삼제품(人蔘製品)의 품질안정성(品質安定性)에 관한 연구(硏究) -4. 홍삼(紅蔘), 삼분(蔘粉) 및 타블렛의 흡습특성(吸濕特性)과 적정수분함량기준(適正水分含量基準) 설정(設定)-)

  • Choi, Jin-Ho;Byun, Dae-Seok;Park, Kil-Dong;Kim, Mu-Nam
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.3
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    • pp.247-250
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    • 1984
  • As a consecutive work on stability for the quality of ginseng products, the sorption properties of red ginseng (RG), red ginseng powder (RGP), and red ginseng powder tablet (RGPT) were studied in order to set the optimum moisture level for them. The levels of moisture at BET monolayer of RGP and RGPT ranged 5.97 to 7.27 % and 4.73 to 5.14 % in wet basis, respectively. Based on these values, the optimum moisture content for both of them would preferably be set at $6.7{\pm}0.7%$ and $4.9{\pm}0.2%$. From the results of storage study on microorganism growth under the maltreated condition, $40^{\circ}C$, and 75% RH, the optimum level of moisture for RG could be extended up to $14.0{\pm}0.5%$, which is much higher than the circulating values of 10.416%.

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Study for Fire Examples of LPG Leakage Including Fuel hose, Injector and Pressure Regulator Connector in Vehicle (자동차 연료호스, 인젝터 및 압력조절기 연결부에서 LP 가스 누출에 의한 화재사례 고찰)

  • Lee, Il Kwon;Kook, Chang Hoo;Suh, Moon Won;Jung, Dong Hwa
    • Journal of the Korean Institute of Gas
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    • v.17 no.3
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    • pp.8-13
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    • 2013
  • The purpose of this paper is to study for fire example by fuel leakage in LPG Vehicle. At first example, the car was repaired the fuel line that was connected with pressure hose between fuel regulator and injector in engine. But the service man was not very tighten with regular torque. At a result, the gas leaked on hot parts of engine. It verified the production of fire by engine heat. At second example, when the repair man, after replacement the injector, inserted the injector in a assembling part of it, he didn't the transform condition of fixing part. Therefore, the tearing phenomenon of O ring producted the controlled leakage of fuel by the injector deflection. It found the fact that the fuel leaked with gap of O ring. At third example. the fuel-cut solenoid valve was lined with pressure regulator unit. But the service man didn't throughly certify the leaked work of connected parts after repaired it. As a result, it certified the fire by engine heating leaked liquefied petroleum gas. Therefore it have to minimize the fire production that the driver should do no problem to throughly manage the fuel system.

Extraction of Carbohydrates and Minerals from Laminaria Using Organic Acid (다시마 뿌리로부터 유기산을 이용한 다당과 미네랄 추출)

  • Chun, Ji Yeon;Han, Cha Seong;Lee, Jung Shik;Kim, Young Suk;Park, Kwon Pil
    • Korean Chemical Engineering Research
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    • v.50 no.2
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    • pp.238-243
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    • 2012
  • Laminaria roots have not been practically used in Korea. In this study, the extraction process of carbohydrates and minerals from Laminaria roots was investigated and the properties of extracted components were measured. Hydrochloric acid generally used in carbohydrate extraction from seaweeds in order to obtain high extraction yield. But in this work, to utilize extracted components as a functional food material, organic acids such as citric acid were used. Organic acid as extraction solvent has low extraction yield compared to strong acids. Therefore optimum condition for maximum yield was investigated in carbohydrate extraction from Laminaria roots using organic acid. We measured the extraction yields of carbohydrate with variation of extraction temperature, extraction time, concentration of organic acid and particle size of samples. The extraction yield increased as the particle size decreased and temperature became high. The extraction yield was 19.0 wt% after 4.0 hours extraction with 0.2 wt% citric acid at $100^{\circ}C$. Potassium concentration was high compared other minerals in extraction solution, that is, the ratio of K/Na was about 3.0. Fucoidan from Laminaria roots had same carbohydrate composition and lower molecular weight compared that of Undaria pinnatifida.

Dynamic Optimization of a Reactive Distillation Column Producing Methyl Acetate (메틸 아세테이트 생산을 위한 반응증류 공정의 동적 최적화)

  • Kim, Jiyong;Kim, Junghwan;Moon, Il
    • Korean Chemical Engineering Research
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    • v.46 no.4
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    • pp.739-746
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    • 2008
  • The aim of this study is finding the optimal design parameters and the optimal operation variables of a reactive distillation column. Different from steady state optimization, dynamic optimization makes it possible considering operation ability as well as design problems at process design step. For performing dynamic optimization, dynamic simulation should be done first. If dynamic simulation is already finished, dynamic optimization can be performed with less effort than that of dynamic simulation.Reactive distillation systems involving reaction and separation in a single unit have the potential to reduce capital and operating costs, particularly when reaction have conversion constraint or when azeotropes exist making conventional separation difficult and expensive. This study here present work on the continuous distillation process, the homogeneous catalyzed esterification of methanol and acetic acid, the synthesis of methyl acetate. Based on an equilibrium stage model of a reactive distillation column a dynamic optimization problem was formulated and solved. And the results were verified by performing dynamic simulation and showing the variation of conversion and purity as the variation of the operation variables. As the results of dynamic optimization, this study found optimal feed ratio, reflux ratio and reboiler duty of this system. And as this study applied it to dynamic simulations the dynamic characteristics of a reactive distillation column are showed under optimal operating condition.

Catalytic Performance for the Production of Synthetic Natural Gas (SNG) on the Commercial Catalyst in Low Hydrogen Concentration; Influence of Steam and CO2 (낮은 수소농도에서 합성천연가스 생산을 위한 상업용 촉매의 반응특성; 스팀과 CO2에 대한 영향)

  • Kang, Suk-Hwan;Kim, Jin-Ho;Kim, Hyo-Sik;Ryu, Jae-Hong;Jeong, Ki-Jin;Yoo, Young-Don;Kim, Kwang-Jun
    • Clean Technology
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    • v.20 no.1
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    • pp.57-63
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    • 2014
  • In this work, we performed the methanation with steam and synthesis gas of a low $H_2/CO$ ratio to develop a process for producing SNG (synthetic natural gas). In this experiment conditions, the water gas shift reaction and the methanation reaction take place at the same time, and insufficient supply of steam might cause the deactivation of the catalyst. Therefore, the reaction characteristics with the amount of steam was performed, and the methanation on syngas containing $CO_2$ of the high concentration were studied. As a result, the temperature in the catalyst bed decreased by the supply of steam, and the methanation and the water gas shift reaction occurred at the same time. Although methane yield slightly decreased at the methanation using syngas containing $CO_2$ of the high concentration, the long-term operation (1,000 h) in the experimental conditions of this study indicates that this condition is suitable for the new commercial scale SNG process.

Development of a Crew Resource Management Training Program for Reduction of Human Errors in APR-1400 Nuclear Power Plant (국내 원자력발전소 인적오류 저감을 위한 Crew Resource Management 교육훈련체계 개발)

  • Kim, Sa-Kil;Byun, Seong-Nam;Lee, Dhong-Hoon;Jeong, Choong-Heui
    • Journal of the Ergonomics Society of Korea
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    • v.28 no.1
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    • pp.37-51
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    • 2009
  • The nuclear power industry in the world has recognized the importance of integrating non-technical and team skills training with the technical training given to its control room operators to reduce human errors since the Three Mile Island and Chernobyl accidents. The Nuclear power plant (NPP) industry in Korea has been also making efforts to reduce the human errors which largely have contributed to 120 nuclear reactor trips from the year 2001 to 2006. The Crew Resource Management (CRM) training was one of the efforts to reduce the human errors in the nuclear power industry. The CRM was developed as a response to new insights into the causes of aircraft accidents which followed from the introduction of flight recorders and cockpit voice recorders into modern jet aircraft. The CRM first became widely used in the commercial airline industry, but military aviation, shipboard crews, medical and surgical teams, offshore oil crews, and other high-consequence, high-risk, time-critical industry teams soon followed. This study aims to develop a CRM training program that helps to improve plant performance by reducing the number of reactor trips caused by the operators' errors in Korean NPP. The program is; firstly, based on the work we conducted to develop a human factors training from the applications to the Nuclear Power Plant; secondly, based on a number of guidelines from the current practicable literature; thirdly, focused on team skills, such as leadership, situational awareness, teamwork, and communication, which have been widely known to be critical for improving the operational performance and reducing human errors in Korean NPPs; lastly, similar to the event-based training approach that many researchers have applied in other domains: aircraft, medical operations, railroads, and offshore oilrigs. We conducted an experiment to test effectiveness of the CRM training program in a condition of simulated control room also. We found that the program made the operators' attitudes and behaviors be improved positively from the experimental results. The more implications of the finding were discussed further in detail.

The Paleovegetation at Dongdo of Is. Dokdo, Korea (한국 독도 동도의 고식생)

  • Yoon, Soon-Ock;Hwang, Sangill
    • Korean Journal of Plant Taxonomy
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    • v.38 no.4
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    • pp.583-599
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    • 2008
  • Dokdo, small island located in the eastern end of the Korean Peninsula, has been an uninhabited island for long time due to long distance from the land. Moreover, the steep slope of volcanic tuff at Dokdo is well drained for high permeability and Dokdo has few plain areas, swamps and very thin soil layers. In this study, pollen analysis at Dokdo was attempted on the profile of organic sandy soil for the first time in Korea owing to the marine climate environment with high humidity and precipitation including snowfalls in spite of unprofitable condition geologically or geomorphologically. While many historical, political researches have been accumulated for territorial problem of Dokdo with Japan, natural scientific researches with field work are not sufficient, and few paleoecological researches have been done. As a result of pollen analysis, the ratio of the NAP(Non-Arboreal Pollen) and spore was higher than AP(Arboreal Pollen), and the vegetation change existed by showing dramatic decrease of AP at the upper layer. AP was composed of almost Pinus, and a little Alnus, Quercus, Betula, Carpinus, Picea and Ulmus. NAP was composed of Gramineae, Compositae, Chenopodiaceae Cyperaceae, Caryophyllaceae, Liliaceae, Umbelliferae, Artemisia, Lobelia, Rumex, Polygonum. Increase of the NAP such as Gramineae, Artemisia and Chenopodiaceae, they would mostly have been transported from the other areas because agricultural activity is impossible at Dokdo. While one reason of Pinus thunbergii decrease could be regarded as environmental change, the other reason would be lumbering of pine trees by human activity which is adapted to Dokdo environment.

The effects of a 24 Weeks of combined exercise programs have on physical configuration, blood components and physical strength for normal and geriatric diseased senior citizens residing in the country side (중소도시 노인들의 24주간 복합운동 프로그램이 성인병 질환자 및 정상인의 신체구성, 혈액성분, 체력에 미치는 영향)

  • Kim, Young-Jin
    • Journal of Digital Convergence
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    • v.11 no.1
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    • pp.431-439
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    • 2013
  • This thesis is to research the before and after changes of physical configurations, blood components and physical strength for normal and geriatric diseased senior citizens at the end of 24 week of combined exercises constructed of aerobic and muscular strength training to create most suitable and effective complex exercise program for geriatric diseased patients. For this experiment 20 normal and 20 geriatric diseased patients in the age of 65 residing in "K" city were selected to carry out the 24 weeks of combined exercises in regularly. The result of the research showed that geriatric patients increased significantly in everything, but normal group showed significant change in only WHR. There was a slight improvement in the blood components for the average participants but it only differed slightly from the diseased participants so there were no major changes reflecting the outcomes from both before and after. After concluding the program both groups displayed positive improvements in stamina but no significant alterations in physical strength., agility, muscle endurance and balance. The positive factors for each groups could be that the norms were able to maintain their health and enhancement in stamina and diseased were able to prevent their condition from worsening. Additionally, over 50 percent of all senior citizens have one or more geriatric diseases but the participation of any physical activity is in the decrease. Henceforth, this is a field that still needs a lot of work and combined exercise programs should be created and followed through so it may enhance in the improvement of health and quality of life as well.