• 제목/요약/키워드: within-individual variation

검색결과 142건 처리시간 0.026초

A phylogenetic analysis of the Korean endemic species Paraphlomis koreana (Lamiaceae) inferred from nuclear and plastid DNA sequences

  • Eun-Kyeong HAN;Jung-Hyun KIM;Jin-Seok KIM;Chang Woo HYUN;Dong Chan SON;Gyu Young CHUNG;Amarsanaa GANTSETSEG;Jung-Hyun LEE;In-Su CHOI
    • 식물분류학회지
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    • 제53권2호
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    • pp.157-165
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    • 2023
  • Paraphlomis koreana (Lamiaceae) was newly named and added to Korean flora in 2014. Paraphlomis belongs to the tribe Paraphlomideae, along with Ajugoides and Matsumurella. However, a recent study has suggested that P. koreana is morphologically similar to Matsumurella chinensis, making them difficult to distinguish from each other. Therefore, we aimed to examine the phylogenetic placement of P. koreana within the tribe and compare its genetic relationship with M. chinensis. We sequenced an additional complete plastid genome for an individual of P. koreana and generated sequences of nuclear ribosomal (nr) DNA regions of internal and external transcribed spacers (ITS and ETS) for two individuals of P. koreana. Maximum likelihood analyses based on two nrDNA regions (ITS and ETS) and four plastid DNA markers (rpl16 intron, rpl32-trnL, rps16 intron, and trnL-F) covering 13 Paraphlomis species and M. chinensis were conducted. Phylogenetic analyses concordantly supported that P. koreana forms a monophyletic group with M. chinensis. Moreover, our study revealed that P. koreana includes nrDNA sequences of M. chinensis as minor intra-individual variants, suggesting that the genetic divergence between the two taxa is incomplete and may represent intraspecific variation rather than distinct species. In conclusion, our findings suggest that the independent species status of P. koreana within Paraphlomis should be reconsidered.

고유시점 재구성을 이용한 방향 변화에 강인한 게이트 인식 (Robust Gait Recognition for Directional Variation Using Canonical View Synthesis)

  • 정승도;최병욱
    • 전자공학회논문지CI
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    • 제41권5호
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    • pp.59-67
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    • 2004
  • 게이트는 사람의 걷는 방법 혹은 그 특성을 나타내는 용어로써, 최근 컴퓨터 비젼 기술을 이용하여 개개인을 분별하기 위한 게이트 특징정보를 추출하고자 하는 연구가 활발히 진행되고 있다 게이트 인식의 경우 카메라가 고정되어 있다고 하더라도 사람이 걸어가는 방향에 따라 영상을 기반으로 추출한 게이트 정보가 달라지는 단점을 가지고 있다. 본 논문에서는 영상내의 정보만을 이용하여, 걷는 방향에 종속적인 게이트 인식의 단점을 보완할 수 있는 방안을 제안한다. 먼저 게이트 영상으로부터 걷는 방향을 찾고, 간단한 연산을 통해 평면 호모그래피를 추정한다. 추정된 호모그래피를 이용하여 고유시점의 영상으로 재구성하면, 게이트 방향에 따른 시점 변화를 보정할 수 있다. 본 논문에서는 게이트 실루엣 영상을 영역별로 나누고 각 영역의 평균 정보와 변화량을 특징으로 사용하여 인식 실험을 진행하였다. 실험 결과, 제안한 방법을 적용했을 경우, 그렇지 않은 경우에 비하여 게이트 방향 변화에 강인한 인식 결과를 보임을 확인하였다.

체외 순환시간의 경과에 따른 Heparin과 Protamine의 적정량에 관한 연구 (Adequate Heparin-protamine Neutralization on using Blood Cardioplegic Solution during Extracorporeal Circulation)

  • 변형섭
    • Journal of Chest Surgery
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    • 제21권2호
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    • pp.203-210
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    • 1988
  • The clinical experience with the activated clotting time[A.C.T.] for the control of heparin and protamine therapy during cardiopulmonary bypass in 40 patients between April, 1987 and September, 1987 is reviewed retrospectively. All of patients used with cold blood potassium cardioplegia for myocardial protection under standard cardiopulmonary bypass, priming and perfusate techniques respectively. This study was divided into 2 groups of patients followed by cardiopulmonary bypass time. Twenty patients, within 60 minutes of cardiopulmonary bypass time[group A] were compared with twenty patients, from 60 to 120 minutes of cardiopulmonary bypass time[group B]. Using blood cardioplegia for myocardial protection, Author observed wide variation of A.C.T. in individual response to initial heparinization[2mg /kg] and no requirement of additional heparin during cardiopulmonary bypass until 120 minutes. Total heparin amount during cardiopulmonary bypass was not related to body weight and body surface area in the both groups. After cardiopulmonary bypass, amounts of protamine for neutralization of heparin were more required in group B.

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Sensitivity analysis of skull fracture

  • Vicini, Anthony;Goswami, Tarun
    • Biomaterials and Biomechanics in Bioengineering
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    • 제3권1호
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    • pp.47-57
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    • 2016
  • Results from multiple high profile experiments on the parameters influencing the impacts that cause skull fractures to the frontal, temporal, and parietal bones were gathered and analyzed. The location of the impact as a binary function of frontal or lateral strike, the velocity, the striking area of the impactor, and the force needed to cause skull fracture in each experiment were subjected to statistical analysis using the JMP statistical software pack. A novel neural network model predicting skull fracture threshold was developed with a high statistical correlation ($R^2=0.978$) and presented in this text. Despite variation within individual studies, the equation herein proposes a 3 kN greater resistance to fracture for the frontal bone when compared to the temporoparietal bones. Additionally, impacts with low velocities (<4.1 m/s) were more prone to cause fracture in the lateral regions of the skull when compared to similar velocity frontal impacts. Conversely, higher velocity impacts (>4.1 m/s) showed a greater frontal sensitivity.

수집종 쑥의 청색색소 카마줄렌 함량 변이 (Variation of Chamazulene Contents in Local Collections of Mugwort (Artemisia spp.))

  • 김현주;채영암
    • 한국약용작물학회지
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    • 제9권3호
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    • pp.181-185
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    • 2001
  • 본 연구에서는 청색색소 카마줄렌을 함유한 쑥 종류를 선발하고, 이 카마줄렌 함량이 집단 내에서 개체에 따라 달라지는 지를 알아보고자 조사 분석한 결과를 요약하면 다음과 같다. 1. 국내 수집종 쑥 5종 쑥(Artemisia indica), 물쑥(Artemisia selegensis), 실제비쑥 (Artemisia japonica var. angustissima), 뺑쑥(Artemisia feddel), 황해쑥(Artemisia argyi)의 정유성분을 분석한 결과 카마줄렌을 함유하고 있는 종은 Artemisia indica이었다. 2. 식물체 내에서 영양생장기와 생식생장기 모두 잎이 화기와 줄기보다 카마줄렌을 많이 함유하고 있었다. 영양생장기에는 상위 엽이 중, 하위 엽보다 2.5배 정도 많은 카마줄렌을, 생식생장기에는 상위 줄기가 중, 하위 줄기보다 5-8배 정도 많이 카마줄렌을 함유하였다. 3. 집단 내 50개체의 개체별 카마줄렌 함량의 변이를 조사한 결과 최소 0.015% 에서 최대 0.113%까지의 큰 변이를 보였으며, 전체 개체의 72%인 36 개체가 0.03% 이상의 카마줄렌을 함유한 것으로 나타났다. 4. 모본과 후대(clones)의 카마줄렌 함량을 비교한 결과 차이가 없었다.

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특정변화패턴 식별을 위한 염기서열 집단간의 다형성 분석 및 시각화 도구 (A Polymorphism Analysis and Visualization Tool for Specific Variation Pattern Identification in Groups of Nucleotide Sequences)

  • 이일섭;이건명
    • 융합정보논문지
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    • 제8권6호
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    • pp.201-207
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    • 2018
  • 유전체는 생명체가 가지고 있는 모든 유전적 정보를 담고 있다. 특정 종 내에서는 개체별로 고유의 특성이 나타나며, 이 특성은 유전체의 염기서열 분석을 통해 확인할 수 있다. 종내 개체들 사이에 조금씩 다른 염기에 대해 유전적 연관성을 규명 짓고, 더 나아가 질병과의 연관성을 찾는 전장유전체 연관분석 연구가 많이 진행되고 있다. 종 내의 조금씩 발생하는 염기변이를 파악하는 것은 개체의 다형성을 파악하기 위해 중요하다. 이 논문에서는 종 내 여러 개체의 염기서열에서 대립형질 빈도의 특정변화패턴을 쉽게 파악할 수 있는 분석 및 시각화 도구를 제안한다. 그리고 수두 대상포진 바이러스의 계대 배양한 pOka strain 염기서열 데이터를 이용해 실험하여 분석과 시각화의 실용성을 보인다. 본 제안도구를 통해 종 내의 대립형질 빈도의 변화를 탐색하고 유전적 요인을 찾는 연구효율의 증진을 기대할 수 있다.

Mitochondrial DNA Sequence Variation of the Mason Bee, Osmia cornifrons (Hymenoptera: Apidae)

  • Kim, Hwa-Young;Lee, Kyeong-Yong;Lee, Sang-Beom;Kim, Se-Ryeon;Hong, Mee-Yeon;Kim, Dong-Young;Kim, Ik-Soo
    • International Journal of Industrial Entomology and Biomaterials
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    • 제16권2호
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    • pp.75-86
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    • 2008
  • In order to understand geographic genetic variation and relationship among populations of the mason bee (Osmia cornifrons Radoszkowsky), which is used as pollinator for apple tree, we sequenced a portion of mitochondrial (mt) COI gene, which corresponds to "DNA Barcode" region (658 bp) from 81 O. cornifrons individuals collected over eight localities in Korea. The sequence data revealed overall moderate to low genetic diversity within species, with a maximum sequence divergence of 0.76%. Geographically, two haplotypes (BAROC01 and BAROC02) were widespread with a frequency of 82.7%, whereas several haplotypes were found in a locality as a single individual, suggesting that haplotype distribution can be summarized as coexistence of a few widespread haplotypes and several regionally restricted haplotypes. Overall, high rate of per generation female migration (Nm=$1.1{\sim}$infinite) and low level of geographic subdivision ($F_{ST}=0{\sim}0.315$) among localities were characteristic. Although two populations (p < 0.026) were genetically subdivided from the remaining localities, no clear polarity was observed. Taken together, the nature of genetic divergence of the mason bee populations is characterized as one that possessing moderate to low genetic diversity, high gene flow, and wide spread haplotypes with ahigh frequency, concordant with the capability of dispersal in connection with the lack of historical biogeographic barriers.

Differences in Temporal Variation of Ground Beetle Assemblages (Coleoptera: Carabidae) between Two Well-Preserved Areas in Mt. Sobaeksan National Park

  • Jung, Jong-Kook;Suk, Sang-Wook;Kim, Byeong-Young;Hong, EuiJeong;Kim, Youngjin;Jeong, Jong-Chul
    • Journal of Forest and Environmental Science
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    • 제33권2호
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    • pp.122-129
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    • 2017
  • Understanding how future climate conditions will be impact on the biodiversity and species composition is important, because biodiversity becomes more important in environment assessment. To understand the biological changes including diversity and species composition over time (temporal variation within a year), the species diversity and composition of ground beetles were investigated in two well-preserved areas in the Sobaeksan National Park using pitfall traps. In addition, relationships between ground beetles and environmental variables were studied by considering temporal variation. We collected 2,146 ground beetle specimens representing 45 species, and individual-based rarefaction curves indicated that similar species richness was found between Geumseon Valley (GV) and Namcheon Valley (NV). The Bray-Curtis matrix comparisons between study sites were characterized by similar ground beetles sample heterogeneity, while temporal variations in abundance, species richness, and ${\beta}-diversity$ of ground beetles showed rather difference over time according to location of study sites. In GV site, minimum temperature was selected as the best predictor for abundance, species richness, and ${\beta}-diversity$ of ground beetles, while those relationships in NV site were more complicated. In conclusion, our study suggests that understanding the different response of ground beetles to climatic variables related to local habitat conditions is important to predict the effect of climate change on biological communities.

Variation in trn-L/trn-V and trn-F/trn-T spacer regions of cpDNA in Abies koreana Wilson and A. nephrolepis Traut./Maxim

  • Kormutak, A.;Hong, Y.-P.;Kwon, H.-Y.;Kim, C.-S.
    • 한국산림과학회지
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    • 제96권2호
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    • pp.131-137
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    • 2007
  • The first evidence has been provided about the variation within trnL-trnV and trnF-trnT spacer regions of cpDNAs in Korean fir and Manchurian fir, revealed by PCR-RFLP analysis. Four cpDNA haplotypes have accordingly been recognized by being analyzed using the trnL-trnV/Tru11 primer-enzyme combination and 3 haplotypes using the trnF-trnT/TagI combination, which exhibited inter and intraspecific variation. A total of 6 cpDNA haplotypes were recognized by pooling the PCR-RFLP variants observed in both combinations. Haplotypes 2 and 3 were common for both species investigated, whereas haplotypes 1, 4, and 5 were detected only in Korean fir and haplotype 6 was detected only in Manchurian fir. Although haplotypes 2 and 3 were common in both species, haplotype 2 was major haplotype for Korean fir and haplotype 3 was one of the 2 major haplotypes for Manchurian fir. Restricted occurrence of haplotype 4 in Mt. Halla and haplotype 5 in Mt. Jiri of the Korean fir may represent the existence of geographic isolation by the sea between them. Diagnostic potential of individual haplotypes in discriminating between the two species as well as between their populations is discussed.

Dose metrology: TLD/OSL dose accuracy and energy response performance

  • Omaima Essaad Belhaj;Hamid Boukhal;El Mahjoub Chakir;Meryeme Bellahsaouia;Siham Belhaj;Younes Sadeq;Mohammed Tazi;Tahar El Khoukhi;Maryam Hadouachi;Khaoula Laazouzi
    • Nuclear Engineering and Technology
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    • 제55권2호
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    • pp.717-724
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    • 2023
  • An essential step in evaluating and comparing the performance of two passive radiation dosimeter types, thermosluminescent (TLD) and optically stimulated luminescence (OSL), used by workers in environments with ionizing radiation for individual radiological monitoring and control of external exposure at various times (cumulative dose for 1 month), is to compare the measured dose accuracy, energy response, and coefficient of variation. In fact this performance study consists in determining the accuracy of both R(10) and R(0.07) which are considered as the ratios of the measured dose (Hp(10) or Hp(0.07)) to the delivered dose (Hp(10) or Hp(0.07)) for each photon energy. The validity of the results of this test is based on the acceptance limits of the ICRP and the international standard IEC-62387. The relative energy response used is normalized to the 137Cs 662 keV energy to find which energy response is closest to the ideal case, and the coefficient of variation that allows to determine the statistical fluctuation of the Hp(10) and Hp(0.07) doses. The results of the accuracy test for the OSL and TLD dosimeters are acceptable because they fall within the ICRP limits. For the energy response, the OSL performs better than the TLD for Hp(10) and Hp(0.07), and for the coefficient of variation, the OSL satisfies the requirements of ISO 62387 for both Hp(10) and Hp(0.07), while the TLD satisfies these requirements only for the measurement of Hp (0.07).