• Journal of Life Science
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• v.18 no.1
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• pp.120-128
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• 2008

The death ligand tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/ Apo1L is a cytokine that activates apoptosis through cell surface death receptors. TRAIL has sparked growing interest in oncology due to its reported ability to selectively trigger cancer cell death. Euphorbiae humifusae Wind has been used in traditional Oriental medicine as a folk remedy used for the treatment of cancer. However, the mechanism responsible for the anticancer effects of E. humifusae not clearly understood. Here, we show that treatment with subtoxic doses of water extract of E. humifusae (WEEH) in combination with TRAIL induces apoptosis in TRAIL-resistant human gastric carcinoma AGS cells. Combined treatment with WEEH and TRAIL induced chromatin condensation and sub-G1 phase DNA content. These indicators of apoptosis were correlated with the induction of caspase activity that resulted in the cleavage of poly (ADP-ribose) polymerase. Combined treatment also triggered the loss of mitochondrial membrane potential. Furthermore, co-treatment with WEEH and TRAIL down-regulated the protein levels of the anti-apoptotic proteins such as Bcl-2, Bcl-xL, XIAP and cIAP-1. Although more study will be needed to examine the detailed mechanisms, this combined treatment may offer an attractive strategy for safely treating gastric adenocarcinomas and the results provide important new insights into the possible molecular mechanisms of the anticancer activity of E. humifusae.

• Journal of Korean Society of Forest Science
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• v.90 no.1
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• pp.90-104
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• 2001

This study is aimed to analyze and to evaluate the revegetation and soil conservation technology in desertification-affected sandy land, resulting from the project of "Studies on the desertification combating and sand industry development". Main native plants for combating desertification : The general characteristics of vegetation distribution in desertified regions are partially concentrated vegetation distribution types including the a) desert plants in low zone of desert or sanddune of depressed basin, b) salt-resistant plants around saline lakes, c) grouped vegetation with Poplar and Chinese Tamarix of freshwater-lakes, saline-lakes and river-banks, d) gobi vegetation of gravel desert and e) grassland and oasis-woods around the alluvial fan of rivers, etc. Generally, Tamarix ehinensis Lour., Haloxylon ammodendron Bunge., Calligonum spp., Populus euphratica Oliver., Elaeagnus angustifolia L., Ulmus pumila L., Salix spp., Hedysarum spp., Caragana spp., Xanthoceras sorbifolia Bunge., Nitraria tangutorum Bobr., Lespedeza bicolor, Alhagi sparsifolia Shap., Capparis spinosa L., Artemisia arenaria DC., etc. are widely distributed in desertified regions. It is necessary for conducting research in the native plants in desertified regions. Analysis of intensive revegetation technology system for combating desertification : In the wind erosion region, the experimental research projects of rational farming systems (regional planning, shelterbelts system, protection system of oasis, establishment of irrigation-channel networks and management technology of enormous farmlands, etc.), rational utilization technology of plant resources (fuelwood, medicinal plants, grazing and grassland management, etc.), utilization technology of water resources (management and planning of watershed, construction of channel and technology of water saving and irrigation, etc.), establishment of sheltetbelts, control of population increase and increased production technology of agricultural forest, fuelwood and feed, etc. are preponderantly being promoted. And in water erosion region, the experimental research projects of development of rational utilization technology of land and vegetation, engineering technology and protection technology of crops, etc. are being promoted in priority. And also, the experimental researches on the methods of utilization of water (irrigation, drainage, washing and rice cultivation, etc.), agricultural methods (reclamation of land, agronomy, fertilization, seeding, crop rotation, mixed-cultivation and soil dressing works, etc.) and biological methods (cultivation of salt-resistant crops and green manure and tree plantation, etc.) for improvement of saline soil and alkaline soil in desertified-lands are actively being promoted. And the international cooperations on the revegetation technology development projects of desertified-lands are sincerely being required.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.288-291
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• 2009

A new commercial rice variety "Donghaejinmi" is a japonica rice (Oryza sativa L.) with lodging resistance and high grain quality. It has been developed by the rice breeding team of Yeongdeog Substation, National Institute of Crop Science (NICS), RDA. This variety was derived from a cross between "Milyang 64" as a resistance source of brown planthopper (Bph) and "Milyang 165" as grain quality source. The donor parent, "Milyang64" has been backcrossed three times with recurrent parent, "Milyang165" and selected by the pedigree breeding method. The pedigree of "Donghaejinmi", designated as "Yeongdeog 41" in 2003, was YR21259-B-B-68-1. It has a short culm length with 69 cm and medium-late growth time. This variety is resistant to stripe virus and moderately resistant to leaf blast disease with durable resistance. It also has tolerance to unfavorable environment such as cold, dried wind and storm. Milled rice kernel of "Donghaejinmi" is translucent, clear in chalkness and good at eating quality in panel test. The merit of this variety is high head rice ratio, which is essential element to produce an article of superior quality rice brand. The yield potential of "Donhaejinmi" in milled rice is about 6.05 MT/ha at ordinary fertilizer level of local adaptability test. This cultivar would be adaptable to Yeongnam inland plains and eastern costal area of Yeongnam province.

• Korean Journal of Breeding Science
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• v.42 no.6
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• pp.638-644
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• 2010

A new rice variety 'Haeoreumi' is a japonica rice (Oryza sativa L.) with lodging tolerance, resistance to rice stripe virus (RSV) and bacterial leaf blight (BLB), and high grain quality. It was developed by the rice breeding team of Yeongdeog Substation, National Institute of Crop Science (NICS), RDA in 2008. This variety was derived from a cross between 'Milyang165' with good grain quality and lodging resistance, and 'Haepyeongbyeo' with wind tolerance in winter season of 2000/2001. A promising line, YR22375-B-B-1, selected by pedigree breeding method, was designated as the name of 'Yeongdeog46' in 2005. 'Yeongdeog46' was released as the name of 'Haeoreumi' in 2008 after the local adaptability test that was carried out at nine locations from 2006 to 2008. 'Haeoreumi' has 74 cm short culm length as and medium maturating growth duration. This variety showed resistance to $K_1,\;K_2$, and $K_3$ races of bacterial blight, and stripe virus and moderate resistant to leaf blast disease with durable resistance, and also has tolerance to unfavorable environment such as cold, dry and cold salty wind. 'Haeoreumi' has translucent and clear milled rice kernel without white core and white belly rice, and good eating quality as a result of panel test. The yield potential of 'Haeoreumi' in milled rice is about 5.58MT/ha at ordinary fertilizer level of local adaptability test. This cultivar would be adaptable to Middle plain, mid-west costal area, and east-south coastal area.

• Research in Plant Disease
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• v.16 no.1
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• pp.101-105
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• 2010

This study was aimed to understand conidial disperse of the pepper anthracnose fungus Colletotrichum acutatum, elapse time for the disease development, and inoculum potentials on infected fruits. Most (99.2%) conidia of the fungus disseminated from inoculum source on the rainy day, while only 0.8% conidia dispersed on the sunny day. Among the conidia 93.3% were caught under 60 cm height at 30 cm distance; however, conidia were detected at 120 cm height at the distance. Relatively susceptible pepper fruits to anthracnose showed first visible symptoms at 4 days after infection under a mimic field condition. However, it seemed that over 10 days are required for the disease to develop on moderately resistant pepper fruits under unfavorable conditions. The number of conidia formed on a lesion was positively correlated with the lesion size ($R^{2}=0.88$). Over 10 millions of conidia were formed at a normal lesion size 1.5 cm in length. In some large coalesced lesions ca. 4cm in length produced over 100 millions of the fungal conidia. Results further confirmed that the rainfall is the key factor for the inoculum disperse of the pepper anthracnose pathogen, Colletotrichum acutatum, and a long distance dissemination is plausible according to rain and wind intensity. Consequently, rain-proof structures are ideal to avoid the disease, and removal of infected fruits and timely chemical spray are indispensible to reduce the inoculum potential in the field.

• Korean Journal of Environment and Ecology
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• v.23 no.1
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• pp.90-98
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• 2009

Every spring, Korea is always plagued by sandy dust from the western region of China and Mongolia. Yellow sand is causing an environmental problem to Japan and far into the American continent, let alone Korea. At present, the western region of China is going under desertification at a great speed due to climatic change and humans' damaging activities. To cope with this, each country including China is considering ecological restoration of deserts through planting. Accordingly, this research conducted a vegetation survey on Baicheng district which is a representative dry land of western China to obtain a basic data for ecological restoration of a desert. The survey revealed that Setaria viridis which invaded an arid land made a succession into Setaria viridis-Cannabis sativa var. fruderalis community together with Artemisia mongolica-Setaria viridis community due to the increase in salt concentration and alkalization subsequent to dryness. It was also found out that there finally formed Artemisia mongolica community on a flat intense in harsh wind and dryness with the continuous worsening of environmental conditions. There appeared a different type of vegetation on hilly districts where sporadic shade could come into being because the air humidity could be available relatively there. Frequently, typically appearing at the whole survey area, the Tributlus terrestris community was found to make succession into Tribulus terrestris-Cleisrogenes squarrosa community due to the aggravation of soil environment. In addition, with the worsening of the environment at hilly districts, there formed Clesirogenes squarrosa community resistant to dryness, salinity in soil and strong alkalinity. Further, there appeared higher plant life totalling to 62 taxa comprising 58 species and 4 varieties with 27 families and 49 genuses at the whole survey area. Among these, Compositae plants excellent in resistance to environment was surveyed the most, accounting for 27%.

• Journal of Korean Society of Forest Science
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• v.94 no.6
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• pp.402-409
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• 2005

This study was undertaken to compare and estimate the severity of pitch canker of individual trees of Pinus rigida and Pinus rigida ${\times}$ P. taeda in two seed orchards in Jeju island, in which the orchards have been damaged by the pitch canker for seven years. Wind-pollinated two-year-old seedlings of P. rigida and P. rigida ${\times}$ P. taeda, in which the seedlings of P. rigida ${\times}$ P. taeda were from seeds of phenotypically selected, uninfected(but untested) trees, were inoculated with the pathogenic fungus, Fusarium circinatum, isolated from P. rigida and P. thunbergii. The virulence of the isolates was also identified. Statistically significant difference was found in 'stem cankers'(SC; ${\chi}^2=7.76$, P=0.05) among 4 plantations of P. rigida ${\times}$ P. taeda of two seed orchards. P. rigida was higher in 'top kill' (TK) and 'branch tip symptoms' (BT) than those of P. rigida ${\times}$ P. taeda. In artificial inoculation tests, mortality of the seedlings from the resistant candidates was 14% higher than that of the seedlings from the susceptible candidates. This result may becaused by unknown pollen trees and/or candidate tree selection based only on phenotype. Two of five fungal isolates, C-6-L(9) and C-6-L(19), showed significantly higher mortality (68% and 60%, respectively) than others, suggesting that these isolates can be used as virulent isolates for a mass artificial inoculation. Resistance candidate seedlings that were selected from this study can be utilized as useful materials for fundamental studies of genetics and biochemistry to breed resistance varieties to pitch canker.