• Parasites, Hosts and Diseases
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• 제54권4호
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• pp.423-429
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• 2016

Cryptosporidium is an important pathogen causing gastrointestinal disease in snakes and is distributed worldwide. The main objectives of this study were to detect and identify Cryptosporidium species in captive snakes from exotic pet shops and snake farms in Thailand. In total, 165 fecal samples were examined from 8 snake species, boa constrictor (Boa constrictor constrictor), corn snake (Elaphe guttata), ball python (Python regius), milk snake (Lampropeltis triangulum), king snake (Lampropeltis getula), rock python (Python sebae), rainbow boa (Epicrates cenchria), and carpet python (Morelia spilota). Cryptosporidium oocysts were examined using the dimethyl sulfoxide (DMSO)-modified acid-fast staining and a molecular method based on nested-PCR, PCR-RFLP analysis, and sequencing amplification of the SSU rRNA gene. DMSO-modified acid-fast staining revealed the presence of Cryptosporidium oocysts in 12 out of 165 (7.3%) samples, whereas PCR produced positive results in 40 (24.2%) samples. Molecular characterization indicated the presence of Cryptosporidium parvum (mouse genotype) as the most common species in 24 samples (60%) from 5 species of snake followed by Cryptosporidium serpentis in 9 samples (22.5%) from 2 species of snake and Cryptosporidium muris in 3 samples (7.5%) from P. regius.

• 한국어병학회지
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• 제30권2호
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• pp.135-148
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• 2017

Pathogen introductions associated with aquatic invasive species threaten ecosystems and biodiversity worldwide. Bigheaded carps (BHC), including Silver Carp Hypophthalmichthys molitrix, Bighead Carp H. nobilis, and their hybrids, are prolific, invasive pests in central US rivers. However, little is known about pathogen effects on invading BHC or how BHC affect the disease risk profile for native fishes in receiving ecosystems. We therefore conducted, from May 2013-December 2014, a systematic pathogen survey for BHC and native fishes in the Wabash River watershed, Indiana, USA. We found Pseudomonas fluorescens, P. putida, and Salmonella enterica DNA in BHC as well as native fishes, although none of these bacteria were exclusively present in BHC. DNA from other bacterial taxa was detected only in native fishes and Common Carp Cyprinus carpio. No gastrointestinal helminths were detected in BHC, although they were common in most native fishes examined. We also conducted in vitro studies on BHC tissues (skin, gill, fin, and fry) and found high sensitivity to Largemouth Bass virus, viral hemorrhagic septicemia virus, and infectious pancreatic necrosis virus. We conclude that BHC are not heavily burdened by bacteria, viruses and parasites in the invaded study ecosystems, although they do harbor native bacteria and show potential for high sensitivity to endemic viruses.

• 한국환경농학회:학술대회논문집
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• 한국환경농학회 2009년도 정기총회 및 국제심포지엄
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• pp.228-239
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• 2009

The observations on climate change show a clear increase in the temperature of the Earth's surface and the oceans, a reduction in the land snow cover, and melting of the sea ice and glaciers. The effects of climate change are likely to include more variable weather, heat waves, increased mean temperature, rains, flooding and droughts. The threat of climate change and global warming on human and animal health is now recognized as a global issue. This presentation is described an overview of the latest scientific knowledge on the impact of climate change on zoonotic diseases. Climate strongly affects agriculture and livestock production and influences animal diseases, vectors and pathogens, and their habitat. Global warming are likely to change the temporal and geographical distribution of infectious diseases, including those that are vector-borne such as West Nile fever, Rift Valley fever, Japanese encephalitis, bluetongue, malaria and visceral leishmaniasis, and other diarrheal diseases. The distribution and prevalence of vector-borne diseases may be the most significant effect of climate change. The impact of climate change on the emergence and re-emergence of animal diseases has been confirmed by a majority of countries. Emerging zoonotic diseases are increasingly recognized as a global and regional issue with potential serious human health and economic impacts and their current upward trends are likely to continue. Coordinated international responses are therefore essential across veterinary and human health sectors, regions and countries to control and prevent emerging zoonoses. A new early warning and alert systems is developing and introducing for enhancing surveillance and response to zoonotic diseases. And international networks that include public health, research, medical and veterinary laboratories working with zoonotic pathogens should be established and strengthened. Facing this challenging future, the long-term strategies for zoonotic diseases that may be affected by climate change is need for better prevention and control measures in susceptible livestock, wildlife and vectors in Korea. In conclusion, strengthening global, regional and national early warning systems is extremely important, as are coordinated research programmes and subsequent prevention and control measures, and need for the global surveillance network essential for early detection of zoonotic diseases.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.2151-2157
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• 2016

Scabraside D, a sulfated triterpene glycoside, was extracted from the sea cucumber Holothuria scabra. It shows anti-proliferation in many of cancer cell lines, but the function and mechanisms of action of scabraside D in human cholangiocarcinoma (HuCCA) have not previously determined. In this study, we investigated the activity of scabraside D on HuCCA cell apoptosis, lymphangiogenesis and metastasis in a nude mouse model. Scabraside D induced signs of apoptosis, such as cell shrinkage, nuclear condensation, nuclear fragmentation and DNA fragmentation on TUNEL assays, while effectively decreasing expression of BCl-2 but increasing caspase-3 gene level expression. Immunohistochemistry revealed that scabraside D significantly reduced lymphatic vessel density (LVD). Moreover, scabraside D treatment significantly decreased VEGF-C, MMP-9 and uPA gene expression, which play important roles in the lymphangiogenesis and invasion of cancer cells in metastasis processes. Quantitative real-time PCR showed that scabraside D significantly decreased iNOS and STAT-3 gene expression. This study demonstrated that scabraside D plays a role in activation of HuCCA tumor apoptosis and inhibition of lymphangiogenesis, invasion and metastasis through decreasing BCl-2, MMP-9, uPA and VEGF-C and increasing caspase-3 expression by suppression of iNOS and STAT-3 expression. Therefore, scabraside D could be a promising candidate for cholangiocarcinoma treatment.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.29-35
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• 2013

A Nodulisporium sp. (Hypoxylon sp.) has been isolated as an endophyte of Thelypteris angustifolia (Broadleaf Leaf Maiden Fern) in a rainforest region of Central America. It has been identified both on the basis of its morphological characteristics and by scanning electron microscopy as well as ITS sequence analysis. The endophyte produces volatile organic compounds (VOCs) that have both fuel (mycodiesel) and use for biological control of plant disease. When grown on potato dextrose agar, the organism uniquely produces a series of ketones, including acetone; 2-pentanone; 3-hexanone, 4-methyl; 3-hexanone, 2,4-dimethyl; 2-hexanone, 4-methyl, and 5-hepten, 2-one and these account for about 25% of the total VOCs. The most abundant identified VOC was 1,8 cineole, which is commonly detected in this group of organisms. Other prominent VOCs produced by this endophyte include 1-butanol, 2-methyl, and phenylethanol alcohol. Moreover, of interest was the presence of cyclohexane, propyl, which is a common ingredient of diesel fuel. Furthermore, the VOCs of this isolate of Nodulisporium sp. were selectively active against a number of plant pathogens, and upon a 24 h exposure caused death to Phytophthora palmivora, Rhizoctonia solani, and Sclerotinia sclerotiorum and 100% inhibition to Phytophthora cinnamomi with only slight to no inhibition of the other pathogens that were tested. From this work, it is becoming increasingly apparent that each isolate of this endophytic Nodulisporium spp., including the Daldina sp. and Hypoxylon spp. teleomorphs, seems to produce its own unique set of VOCs.

• Parasites, Hosts and Diseases
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• 제60권6호
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• pp.423-427
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• 2022

The long-tailed goral, Naemorhedus caudatus (Mammalia: Bovidae), is one of the endangered animals in the Republic of Korea (Korea). Sarcoptic mange mites infested in diverse species of mammals, including humans, but no case has been reported in long-tailed gorals. We report 2 cases of mange mite, Sarcoptes scabiei, infestation in longtailed gorals. Mange mites were sampled in the skin legions of the 2 long-tailed gorals, which were rescued in 2 different regions, Uljin-gun, Gyeongsangbuk-do and Cheorwon-gun, Gangwon-do, Korea. Our results showed that the ectoparasite was the itch mite that burrowed into skin and caused scabies on the morphological inspection and placed within the phylogenetic relations of the species. The present study confirmed for the first time in Korea that mange mites are pathogenic scabies of long-tailed goral. Closer surveillance of this pathogenic ectoparasite in zoonotic and infectious ecosystems is warranted.

• 한국동물위생학회지
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• 제46권4호
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• pp.293-302
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• 2023

This study provides a comprehensive analysis of the epidemiological trends and challenges in managing tuberculosis (TB) in livestock in Korea from 2013 to 2022. Tuberculosis, caused by the Mycobacterium tuberculosis complex, is a significant zoonotic disease affecting cattle, deer, and other domesticated animals. Despite the initiation of a test-and-slaughter eradication policy in 1964, TB has continued to persist in Korean livestock, particularly in cattle and deer. This study used data from the Korea Animal Health Integrated System and provincial animal health laboratories to analyze TB incidence in various livestock including different cattle breeds and deer species. The results from 2013 to 2022 showed a peak in TB cases in 2019 with a subsequent decline by 2022. The study highlighted a significant incidence of TB in Korean native cattle and the need for amore inclusive approach towards TB testing and control in different cattle breeds. Additionally, the study underscored the importance of addressing TB in other animals such as goats, wildlife, and companion animals for a holistic approach to TB eradication in Korea. The findings suggest that while the test-and-slaughter strategy has been historically effective, there is a need for adaptation to the current challenges, and learning from successful eradiation stories on other countries like Australia. A collaborative effort involving an expanded surveillance system, active private sector participation, and robust government support essential for the efficient eradication of TB in livestock in Korea.

• 한국임상수의학회지
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• 제24권1호
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• pp.1-4
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• 2007

고도병(High altitude disease)의 위험에도 불구하고, 고냉지에서 한우를 사육할 경우, 감염이나 스트레스 위험이 낮아서 한우의 육질을 개선하는데 도움이 된다고 한다. 하지만 어떤 고도가 고도병의 위험성이 없이 최적의 사육환경을 제공하는지에 대한 연구가 부족한 실정이다. 따라서 본 연구에서는 3가지 다른 고도(200m, 400m and 800m)에서 사육되고 있는 한우를 대상으로 혈액 검사, 혈청 생화학 검사 및 스트레스와 관련된 호르몬 검사를 실시하였다. 본 연구에서 적혈구 수치와 혈색소의 농도는 800m고지에서 사육된 한우에서 가장 높았고, 200m고지에서 사육된 한우에서 가장 낮게 나타났다. 이러한 결과는 저산소증에 따른 골수와 적혈구 신생 자극 반응에 기인한 것으로 보인다. 혈중의 AST, BUN과 cortisol농도는 800m고지에서 사육된 한우에서 가장 낮게 측정되었으며, 전반적인 건강상태도 200m지대에서 사육된 한우보다 800m지대에 사육된 한우 집단이 건강 상태가 양호한 것으로 나타났다. 이 결과는 한우의 건강에 영향을 주는 스트레스 요인이 800m고지에서 최소로 나타남을 의미하는 것 같다.

• 환경영향평가
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• 제31권5호
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• pp.310-320
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• 2022

본 연구는 국내 최대 소셜 네트워크인 블로그 글을 대상으로 텍스트마이닝 및 네트워크 분석을 통해 COVID-19 발생 후 '박쥐'에 대한 대중들의 인식 변화를 파악하였다. 국내에서 COVID-19 발생전 2019년부터 2020년까지 9,241건의 네이버 블로그 글을 수집하였다. 수집된 자료는 파이썬(Python)과 NetMiner 4.3.2으로 분석하였고, 시기별로 도출된 키워드와 키워드 간 연관성을 통해 박쥐에 대한 대중들의 인식을 심층적으로 분석하였다. 분석결과, 2020년 박쥐 키워드의 출현 빈도는 2019년에 비해 25배 이상 증가하였고, 중심성 수치 또한 3배 이상 증가되었다. 네트워크 분석 결과, '박쥐'에 대한 인식은 COVID-19 발생전과 후 차이를 나타냈다. COVID-19 이전에 박쥐는 야생동물의 한 종(Species)으로 인식되는 경향성이높았던 반면, COVID-19 발생 초기인 2020년 상반기에는 전염병 및 건강 분야와 연관시켜 인간사회를 위협할 수 있는 존재로 강하게 인식하였고, 하반기에는 생태 및 문화 유형 비중이 높아지면서 박쥐에 대한 관심영역이 확장된 것을 확인하였다. 본 연구는 COVID-19 발생 이후 질병 숙주로서 박쥐의 잠재적인 영향에 대한 대중들의 관심과 인식 변화에 대한 정보를 제공함으로써 질병연구의 확장과 공중보건 관리, 미래감염병 대응을 위한 방향을 제시하였다.

• 한국환경생태학회지
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• 제33권1호
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• pp.9-15
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• 2019

우제목은 짝수 개의 발굽을 갖는 포유동물로 다양한 종이 전 세계적으로 광범위하게 서식하고 있다. 최근 국내에서는 멧돼지, 고라니와 같은 야생 우제목 동물에 의한 농작물 피해, 로드킬 등의 급증과 산양, 사향노루 등 일부 종의 개체수 급감으로 사회적 관심을 받고 있다. 그러나 이러한 사회적 관심에도 불구하고 우제목 관련 국내 연구는 매우 부족하며, 국내 우제목의 연구 동향 분석도 이루어지지 않아 실질적인 문제점을 파악하는데 어려움이 있다. 최근 연구 동향분석에 있어 텍스트마이닝과 동시출현단어분석은 연구 문헌들에서 나타나는 주요 단어들을 추출하고 단어들 간의 연관성을 정량화하는데 활용되고 있으며, 연구 주제의 분류에 있어 객관성을 증가시킨다. 본 연구에서는 텍스트마이닝과 동시출현단어분석을 통해 한국, 중국, 일본 3국의 우제목 연구 논문을 분석하고 국가별 연구 주제를 비교하여, 국내 우제목 연구에서의 부족한 점과 향후 필요한 점을 알아보고자 하였다. 각 국가별로 우제목과 관련된 연구 논문을 검색하여 수집한 665편의 논문들에 대한 텍스트마이닝 결과, 총 199개 단어가 추출되었다. 추출된 단어들에 대한 동시출현단어분석 결과 3개의 단어군이 형성되었다. 각 단어군에 포함된 단어들을 살펴본 결과, 단어군1은 "서식환경/생태", 단어군2는 "질병", 단어군3은 "보전유전학/분자생태"와 관련 있는 것으로 판단된다. 국가별로 각 단어군의 비율을 살펴본 결과, 중국과 일본은 비교적 고른 단어군 비율을 나타낸 반면, 한국은 "질병"과 관련된 단어군2의 비율이 69%로 상당히 큰 편중을 나타내었다. 연도에 따른 각 단어군별 단어수 회귀 분석 결과에서도 중국과 일본은 3개의 단어군에 해당하는 단어수가 시간 경과에 따라 비교적 고르게 증가하였지만, 한국은 단어군2의 증가율이 나머지 단어군의 5배 이상을 나타냈다. 국내 우제목 연구는 중국과 일본에 비해 질병과 관련된 연구 위주로 진행된 것으로 판단되며, 서식 특성, 행동, 분자생태를 포함한 연구는 매우 적게 수행된 것으로 판단된다. 향후 국내 야생 우제목 동물에 의한 피해 조절과 멸종위기종 보호를 위한 합리적인 정책 수립을 위해, 야생 우제목에 대한 생태 연구를 집중적으로 실시하여 기초생태 자료를 축적시켜 나가야 할 것이다.