• Title/Summary/Keyword: wildlife disease

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Toxoplasmosis in a Pet Peach-Faced Lovebird (Agapornis roseicollis)

  • Cooper, Madalyn K.;Slapeta, Jan;Donahoe, Shannon L.;Phalen, David N.
    • Parasites, Hosts and Diseases
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    • v.53 no.6
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    • pp.749-753
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    • 2015
  • Toxoplasma gondii atypical type II genotype was diagnosed in a pet peach-faced lovebird (Agapornis roseicollis) based on histopathology, immunohistochemistry, and multilocus DNA typing. The bird presented with severe neurological signs, and hematology was suggestive of chronic granulomatous disease. Gross post-mortem examination revealed cerebral hemorrhage, splenomegaly, hepatitis, and thickening of the right ventricular free wall. Histologic sections of the most significant lesions in the brain revealed intralesional protozoan organisms associated with malacia, spongiform changes, and a mild histiocytic response, indicative of diffuse, non-suppurative encephalitis. Immunohistochemistry confirmed the causative organisms to be T. gondii. DNA isolated from the brain was used to confirm the presence of T. gondii DNA. Multilocus genotyping based on SAG1, altSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico markers demonstrated the presence of ToxoDB PCR-RFLP genotype #3 and B1 gene as atypical T. gondii type II. The atypical type II strain has been previously documented in Australian wildlife, indicating an environmental transmission route.

Perspectives provided by leopard and other cat genomes: how diet determined the evolutionary history of carnivores, omnivores, and herbivores

  • Kim, Soonok;Cho, Yun Sung;Bhak, Jong;O'Brian, Stephen J.;Yeo, Joo-Hong
    • BMB Reports
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    • v.50 no.1
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    • pp.3-4
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    • 2017
  • Recent advances in genome sequencing technologies have enabled humans to generate and investigate the genomes of wild species. This includes the big cat family, such as tigers, lions, and leopards. Adding the first high quality leopard genome, we have performed an in-depth comparative analysis to identify the genomic signatures in the evolution of felid to become the top predators on land. Our study focused on how the carnivore genomes, as compared to the omnivore or herbivore genomes, shared evolutionary adaptations in genes associated with nutrient metabolism, muscle strength, agility, and other traits responsible for hunting and meat digestion. We found genetic evidence that genomes represent what animals eat through modifying genes. Highly conserved genetically relevant regions were discovered in genomes at the family level. Also, the Felidae family genomes exhibited low levels of genetic diversity associated with decreased population sizes, presumably because of their strict diet, suggesting their vulnerability and critical conservation status. Our findings can be used for human health enhancement, since we share the same genes as cats with some variation. This is an example how wildlife genomes can be a critical resource for human evolution, providing key genetic marker information for disease treatment.

A survey on gastrointestinal parasites of Korean water deer (Hydropotes inermis agyropus) in Chungbuk province (충북지역 야생 한국고라니의 내부기생충 감염률 조사)

  • Choe, Seong-Jun;Na, Ki-Jeong;Jee, Cha-Ho
    • Korean Journal of Veterinary Service
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    • v.34 no.3
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    • pp.259-263
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    • 2011
  • Wildlife has been recognized that has important role as reservoir hosts of disease affecting both people and livestocks. However, information intestinal helminths of Korean water deer were limited. We obtained 20 feces from Korean water deers that were transferred to veterinary hospital in Chungbuk National University from May 2010 to June 2011. Parasite ova were collected using saturated zinc sulfate floatation method. Parasite eggs were examined and measured using light microscope. The identification of parasitic eggs was done by morphological characters. The overall infection rate of parasite was 85% (n=17). The eggs were observed as being Nematodirus spp. (n=14), strongyles egg (n=11), Capillaria spp. (n=9), Trichuris spp. (n=7), Eimeria spp. (n=4), Ogmocotyle spp. (n=3), and Strongyloides (n=1). Mixed infection rate was observed as being single 15% (n=3), double 20% (n=3), triple 15% (n=3), quadruple 30% (n=6), and quintuple 5% (n=1). The Ogmocotyle spp. was first reported in Korea.

Complete genome of methicillin resistant Staphylococcus epidermidis Z0117SE0041 isolated from human nasal mucosa (사람 코점막에서 분리된 메티실린 내성 Staphylococcus epidermidis Z0117SE0041의 유전체 염기서열)

  • Patil, Kishor Sureshbhai;Oh, Jae-Young;Han, Jae-Ik;Song, Wonkeun;Park, Hee-Myung;Chae, Jong-Chan
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.474-476
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    • 2018
  • Methicillin resistant Staphylococcus epidermidis Z0117SE0041 was isolated from nasal mucosa of human who raised companion dog. The complete genome of strain Z0117SE0041 consists of a 2.5 Mb chromosome and three circular plasmids with about 47, 36, and 11 kb in size, respectively. Since strain Z0117SE0041 raises concerns due to its potential to cause a disease and disseminate antibiotic resistance, further genome analysis is required in detail.

Infection by a Filarial Nematode from the Family Onchocercidae in the Wild Bird Anas falcata

  • Kim, Young Ji;Jang, Jin Ho;Kim, Min Chan;Park, Young-Seok;Kim, Hye Kwon
    • Proceedings of the National Institute of Ecology of the Republic of Korea
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    • v.3 no.4
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    • pp.221-226
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    • 2022
  • A filarial nematode was found in a blood sample of an Anas falcata individual collected in South Korea in 2018. Phylogenetic analysis based on partial cytochrome C oxidase subunit I (COI) sequences placed the nematode as a novel genus of the family Onchocercidae and as closely related to Mansonella species, Chandlerella quiscali, and filarial nematodes recently reported in avian species. However, different phylogenetic relationship was observed in the NADH dehydrogenase subunit 5 and 12S rRNA-based phylogenetic trees, which might indicate the filarial nematode found in this study was not defined to belong to the known specific genera of the family Onchocercidae. The screening of 105 additional avian blood samples retrieved only one 12S rRNA-targeting polymerase chain reaction (PCR)-positive sample, which indicates that filarial nematode infection is rare in wild birds or that it occurs below the detection limit of PCR in blood samples. Nevertheless, considering the recent findings about ancient interactions between birds and human pathogenic filarial nematodes and their pathogenic potential in several avian species, additional exploration of novel filarial nematodes in wild birds remains necessary.

Prevalence of Dirofillaria immitis in Raccoon Dogs (Nyctereutes procyonoides) in Korea (한국 너구리(Nyctereutes procyonoides)의 심장사상충감염에 대한 유병률)

  • Nam, Hyo-Seung;Kim, Jong-Taek;Yang, Dong-Keun;Hyun, Changbaig
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.453-455
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    • 2013
  • Heartworm infection is a fatal disease causing heart failure and pulmonary diseases in dogs. This heartworm infection can also occur in wild carnivores including Raccoon dogs. Recent study found that relatively high prevalence rate in wild Raccoon dog population. Therefore, this study was designed to evaluate the prevalence rate of D. immitis in free-roaming Raccoon dogs and the recovery rate of microfilariae in infected Raccoon dogs in Korea. Overall prevalence rate of D. immitis in Korean Raccoon dogs was 17.8%. Prevalence rate in male Raccoon dogs was 21.8%, while that in female Raccoon dogs was 12.8%. Microfilariae were not detected in 17 Raccoon dogs having positive in heartworm antigen test. Our study result suggested that the prevalence rate of D. immitis in Korea is twice higher than that of Japan. In addition, microfilaremia is rare in Raccoon dogs as commonly noticed in cats.

Effect of culture-promoting ingredients (CPI-107) on the culture of Mycobacterium tuberculosis (결핵균 배양에 대한 배양촉진물질(CPI-107)의 효과)

  • Seung Cheol Kim;Sezim Monoldorova;Bo-Young Jeon
    • Korean Journal of Veterinary Service
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    • v.46 no.1
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    • pp.29-34
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    • 2023
  • Mycobacterium tuberculosis complex (M. tuberculosis complex) is a causative agent of contagious chronic disease in a wide range of mammalian hosts, mainly cattle, goat, pigs, wildlife, and humans. The definite diagnosis of tuberculosis is made based on culture of M. tuberculosis, but it takes a long time. In the present study, we analyzed whether the detection time of M. tuberculosis could be reduced when cultured in the medium containing the culture-promoting ingredients-107 (CPI-107) using the BacT/Alert 3D system, an automatic culture system. The time to detection (TTD) tended to decrease as the added concentration of CPI-107 increase. In the case of low numbers of M. tuberculosis, it decreased by 21.0% at 1.2 mg/mL of CPI-107 and by 15.9% in the case of high numbers of M. tuberculosis. In the culture using clinically isolated M. tuberculosis strains, the shortening of the culture time by CPI was more evident. In conclusion, the detection time of M. tuberculosis was shortened in the medium added with CPI-107, and this could be used for isolation, culture and drug susceptibility test of M. tuberculosis.

U.S. Forest Service Research : Its Administration and Management

  • Krugman, Stanley L.
    • Journal of Korean Society of Forest Science
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    • v.76 no.3
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    • pp.243-248
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    • 1987
  • The U.S. Forest Service administers the world's largest forestry research organization. From its modest beginning in 1876, some 30 years before the United States national forest system was established, the research branch has devoted its effort to meet current and future information needs of the forestry community of the United States, not just for the U.S. Forest Service. The research branch is one of three major administrative units of the U.S. Forest Service. The others being the National Forest System and State and Private Forestry. Currently the National Forest System comprises 155 national forests, 19 national grasslands, and 18 utilization projects located in 44 states. Puerto Rico, and the Virgin Islands. The National Forest System manages these areas for a large array of uses and benefits including timber, water, forage, wildlife, recreation, minerals, and wilderness. It is through the State and Private Forestry branch that the U.S. Forest Service cooperates and coordinates forestry activities and programs with state and local governments, forest industries, and private landowners. These activities include financial and technical assistance in disease, insect, and fire protection ; plan forestry programs ; improve harvesting and marketing practices ; and transfer forestry research results to user groups. Forestry research is carried out through eight regional Forest Experiment Stations and the Forest Product Laboratory. Studies are maintained at 70 administrative sites, and at 115 experimental forest and grasslands. All of the current sciences that composed modern forestry are included in the research program. These range from forest biology (i. e. silviculture, ecology, physiology, and genetics) to the physical, mathematical, engineering, managerial, and social sciences. The levels of research range from application, developmental, and basic research. Research planning and priority identification is an ongoing process with elements of the research program changing to meet short-term critical information needs(i. e. protection research) to long-term opportunities(i. e. biotechnology). Research planning and priority setting is done in cooperation with National Forest Systems, forest industries, universities, and individual groups such as environmental, wilderness, or wildlife organizations. There is an ongoing review process of research administration, organization, and science content to maintain quality of research. In the U.S. Forest Service the research responsibility is not completed until the new information is being applied by the various user group : I. e. technology transfer program. Research planning and development in the U.S. Forest Service is a dynamic activity. Porgrams for the year 2000 and beyond are now in the planning stage.

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Investigation of Variation in Bacterial Community Structure in Endangered Korean Fir Tree by Habitats (멸종위기종 구상나무 서식지별 세균 군집 구조 변이 조사)

  • Young Min Ko;Geun-Hye Gang;Dae Ho Jung;Youn-Sig Kwak
    • Research in Plant Disease
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    • v.30 no.2
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    • pp.165-175
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    • 2024
  • The Korean fir tree (Abies koreana), an endemic species of South Korea, is experiencing a severe decline in population due to climate change. Studies on the conservation of Korean fir have been extensive, yet research regarding its correlation with rhizosphere bacterial communities remains scarce, warranting further investigation. In this study, metagenome amplicon sequencing targeting the 16S rRNA V4 region was conducted to examine the presence of specific bacterial communities in Korean fir and to investigate potential differences based on habitat types (rhizosphere of native or cultivated trees, soil of dead trees, and bulk soil) and seasonal variations (April, June, September, November). Here we show that although we could not identify specific taxa highly specifically with Korean fir, the rhizosphere bacterial community in native trees exhibited less variability in response to seasonal changes compared to that in bulk soils. Suggesting the establishment of relatively stable bacterial populations around the Korean fir natural habitat. Further research on other types of rhizosphere and/or microbes is necessary to investigate the distinct relationship of Korean fir with microbial communities.

Evaluation of Electrocardiographic Index in Healthy Raccoon Dogs (Nyctereutes procyonoides) (건강한 너구리(Nyctereutes procyonoides)들의 심전계 지표에 대한 평가)

  • Hong, Won-Woo;Kim, Jong-Taek;Yang, Dong-Keun;Nam, Hyo-Seung;Hyun, Changbaig
    • Journal of Veterinary Clinics
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    • v.30 no.6
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    • pp.456-458
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    • 2013
  • The aim of this study is to evaluate the reference value for electrocardiogram in healthy captive raccoon dogs. Forty-one free-ranging adult raccoon dogs rescued from Wildlife rescue centre, Kangwon National University were enrolled in this study. The 6-lead electrocardiogram was obtained in all raccoon dogs without any chemical restraints. The mean heart rate was $146.10{\pm}43.31$ beats/min (95% confidence interval 132.84~159.36 beats/min). The mean respiration rate was $35.73{\pm}11.56$ breaths/min (95% confidence interval 32.19~39.27 breaths/min). The mean systolic blood pressure was $136{\pm}29.26$ mmHg (95% confidence interval 127.99~145.91 mmHg). Electrocardiographical features were also evaluated in all raccoon dogs. The mean duration and amplitude of P-wave were $38.2{\pm}4.0$ ms (range 28-40 ms) and $0.128{\pm}0.039$ mV (range 0.09~0.20). The mean duration and amplitude of QRS complexes were $48.5{\pm}7.2ms$ (range 36-60 ms) and $1.330{\pm}0.650$ mV (range 0.15~2.30). The range of the mean electrical (QRS) axis was $-91^{\circ}{\sim}+96^{\circ}$ ($10^{\circ}{\sim}60^{\circ}$; 95% of confidence interval). The mean corrected QT (QTc) interval was $273.7{\pm}32.7ms$ (range 212-333 ms), while the mean PR interval was $76.1{\pm}10.0ms$ (range 50-82 ms). To the authors' knowledge, this is the first study to provide references in electrocardiogram (ECG) in healthy captive raccoon dogs.