• Korean Journal of Veterinary Research
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• v.41 no.4
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• pp.467-475
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• 2001

Cardiac arrest, hypoxia, shock or seizure has been known to induce cerebral ischemia. This study was designed to investigate the effect of ischemia on hippocampal pyramidal layer induced by transient bilateral occlusion of the common carotid arteries. Mature Mongolian gerbils were sacrificed at days 2, 4, and 7 after carotid occlusion for 10 minutes. Sham-operated gerbils of control group were subjected to the same protocol except for carotid occlusion. During operation for ischemia, body temperature was maintained $37{\pm}0.5^{\circ}C$ in all gerbils. Paraffin-embedded brain tissue blocks were cut into coronal slices and stained with H-E stain or immunostain by TUNEL method. Neurons with the oval and prominent nucleus and without the eosinophilic cytoplasm in the subfield of hippocamapal pyramidal layer were calculated as to be viable neurons. Their chromatins were condensed or clumped. Their nuclei appeared multiangular or irregularly shrinked. The width of the pyramidal layer was reduced due to the loss of nuclei. At day 2 after reperfusion, some neurons in the CA1 subfield were slightly eosinophilic. But most neurons in the CA2 subfield were strongly eosinophilic. At day 4 day, most neurons in the CA1 subfield were severely damaged and at day 7 day, only a few survived neurons were observed. Survived neurons per longitudinal 1mm sector in the CA1, CA2, CA3, and CA4 subfields of pyramidal layer were investigated. At day 2, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 104.5/mm (54.3%), 51.0/mm (33.8%), 105.5/mm (85.6%), and 124.3/mm (93.5%) compared to the nonischemic control group, respectively. At day 4, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfields were 3.2/mm (1.7%), 51.5/mm(34.2%), 95.3/mm (77.4%), and 112.5/mm (84.6%), respectively. At day 7, the mean numbers of pyramidal neurons in CA1, CA2, CA3, and CA4 subfiedls were 0.8/mm (0.4%), 5.7/mm(3.8%), 9.8/mm (8.0%), and 5.0/mm (3.7%), respectively. The mean numbers of apoptotic positive neurons in the CA1 subfield at day 2, 4, and 7 after reperfusion were 67.8/mm, 153.2/mm and 123.7/mm, respectively. These results suggest that the transient cerebral ischemia cause severe damages in most neurons at day 7 and that the prosminent apoptotic positive neurons in hippocampal pyramidal layer are the delayed neuronal death induced by ischemia.

• Korean Journal of Organic Agriculture
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• v.11 no.3
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• pp.65-73
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• 2003

This study was conducted to investigate the effect of microbial liquid manure(MLM) on the growth and yield characteristics of Altari radish (Raphanus sativus L.). MLM 1,000mg$.$L$^{-1}$ MLM 2,000mg$.$L$^{-1}$ and MLM 1,000mg L$^{-1}$ + microbial fermentation compost(MFC) 600kg/10a were treated. MLM was sprayed on leaves at 10, 20, and 40 days after planting. Leaf length and width of radish in all microbial fertilizer treatments were more vigorous than that of control significantly. Number of leaves was great in order of 1,000 mg$.$L$^{-1}$ MLM+600kg/10a MFC 〉2,000mg$.$L$^{-1}$ MLM〉1.000mg$.$L$^{-1}$ MLM. SPAD reading value was increased with treatments of 2,000mg$.$L$^{-1}$ MLM and 1,000mg$.$L$^{-1}$ MLM+600kg/10a MFC. Root length and weight of all the microbial fertilizer treatments tended to increase compared with control, and 1,000mg$.$L$^{-1}$ MLM+600kg/10a MFC treatment was the most effective.

• Journal of Environmental Science International
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• v.22 no.1
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• pp.17-23
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• 2013

In order to evaluate the effect of various organic soil conditioner ratios on the soil moisture content and growth of Cotoneaster horizontalis, a container type for wall-planting experiment was conducted in a greenhouse at Konkuk University under non-irrigation. The experimental planting grounds were prepared with different organic soil conditioner ratios ($A_1L_0$, $A_8L_1$, $A_4L_1$ $A_2L_1$ and $A_1L_1$), and a drought-tolerant ornamental variety of Cotoneaster horizontalis was planted. The change in soil moisture content, plant height, number of branches, number of dead leaves, number of leaves, number of shoots, length of node, length of leaf, width of leaf, root-collar caliper, chlorophyll content, and survival rate were investigated, from April to Jun 2010. The results of soil moisture content measurements were analyzed with weight units in the container type for wall-planting during the dry summer season. The soil moisture contents were significantly enhanced in the container type for wall-planting in increasing order as the amount of soil conditioner level was increased ($A_1L_1$ > $A_2L_1$ > $A_4L_1$ > $A_8L_1$ > $A_1L_0$). Compared to the control treatment application (amended soil with 100% + organic soil conditioner 0%), the highest plant growth was observed in the treatment of $A_1L_1$ application (amended soil with 50% + organic soil conditioner 50%). However, the differences between the organic soil conditioner ratio treatments of $A_1L_1$, $A_4L_1$, and $A_8L_1$ organic soil conditioner application were mostly not significant. The survival rate increased with the increasing application of organic soil conditioner, but in the control treatment application all the plants died. The experimental results from clearly demonstrated that the organic soil conditioner improved the survival rate more than the growth of Cotoneaster horizontalis. Therefore, Cotoneaster horizontalis is expected to be a highly valuable shrub for green wall systems, when considered for us in integration system or for increasing soil water contents in planting grounds.

• Journal of Bio-Environment Control
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• v.20 no.2
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• pp.139-143
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• 2011

This study was conducted to investigate correlation between external factors of chicory root and chicon fresh weight, whereby, to provide basic information of external yardstick of producing standardized chicon. For correlation between external factors (root diameter, root weight) of chicory root and chicon fresh weight were highly correlated. The relationship between external factors (root diameter, root weight) of chicory root and chicon fresh weight, root diameter was strongly affecting chicon fresh weight due to significant at its regression coefficient ($6.06^{***}$). In order to verity this correlation, the root diameter (x) of chicory that were 3 different varieties were based on the 4 different root diameter treatments, such as x ${\leq}$ 30 mm, 30 < x ${\leq}$ 40 mm, 40 < x ${\leq}$ 50 mm and x > 50 mm. The chicon production increased as root diameter increased and the chicon fresh weight, chicon width, and the number of leaves increased as well. Therefore, the measure of root diameter of chicory instead of the internal factors (carbohydrate, and so on.) can be used to predict the chicon fresh weight.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.20-25
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• 2008

This study was conducted to develop the industrial producing technology of sprout vegetables using the cut twigs of woody plants. We have cut the twigs of wild mulberry tree on 13 April,2006 to examine the sprouting rate as affected by the storage conditions on relative humidity of $40{\sim}50%$ and $80{\sim}90%$ under $4{\sim}6^{\circ}C$ temperature. And we have also investigated the sprouting growth following those storage period of twigs cut with $1{\sim}3$ buds. The longer storage periods, the less survival rate of wild mulberry twigs cut. The sprouting rate of cut twigs gathered on the day which had not stored at low temperature was 97.4%. And The sprouting rate at the relative humidity of $40{\sim}50%$ after storing at low temperature for 122 days were 34.2%, and that of at $80{\sim}90%$ relative humidity was 85.7%. The longer storage periods, the more the number, length and width of sprouts. Therefore, the twigs of a wild mulberry trees cut with $1{\sim}3$ buds were able to produce the sprout vegetables under the condition of temperature $4{\sim}6^{\circ}C$ and relative humidity $80{\sim}90%$.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.9-13
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• 2008

The wasabi was planted in glasshouse to investigate the effect shade level (0, 10, 30, 70%) on growth and photosynthetic response. The net photosynthetic rate and stomatal conductance were the highest under 10% shading rate in greenhouse. The lowest transpiration rate was in the non-shading treatment. 70% shading rate significantly reduced in photosynthetic characteristics. The number of leaf was decreased with increasing of shading rate. Leaf and petiole weight was the highest 10% shading and then followed by the non-shading treatment. Number of leaves, leaf, petiole and total weight were severely declined in 70% shading treatment. Shading rate (0%, 10%, and 30%) did not significantly influenced on the leaf length, leaf width and root growth. 10% shading level was the most effective for wasabi leaf production in hydroponics.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.1-8
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• 2008

Four cover plants such as Phlox subulata, Glechoma hederacea var. longituba, Sedum middendorffianum and Saxifraga laciniata were evaluated to investigate the effect of companion planting on reducing soil erosion in Chinese cabbage cultivated highland. The experiment was conducted using lysimeters of $5\;m{\times}2\;m$ (length$\times$width) with 5, 15 and 30% slopes. Companion plants except Sedum middendorffianum did not interface with growth of Chinese cabbage Glechoma hederacea var. longituba and Sedum middendorffianum grew faster than Phlox subulata and Saxifraga laciniata in the early growth stage suer transplanting, resulting in fast ground covering, but the ground covering by Phlox subulata and Saxifraga laciniata was delayed because growth suppression by high air temperature during summer season. Soil erosion became severe as increasing degree of slope. Assessments of the four cover plants were conducted in relation to soil conservation characteristic of scenery, endurance to the environment stress, plant growth and weed suppression. From the assessments, Phlox subulata was superior to other intercropping crops tested for reducing soil erosion in highland cultivation of Chinese cabbage.

• Journal of Bio-Environment Control
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• v.22 no.3
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• pp.228-233
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• 2013

This study was conducted to examine the effect of light intensity and photoperiod of white LEDs as the artificial light source on the growth of leaf lettuce (Lactuca sativa L.) 'Seonhong Jeokchukmyeon' in a closed-type plant production system. Seedlings, transplanted at a density of $20cm{\times}20cm$ in a completely randomized design, were grown under white LEDs (FC Poibe Co. Ltd., Korea), at one of the 3 light intensities (100, 200, or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), and each with one of 3 photoperiods [12/12, 18/6, or 24/0 (Light/Dark)]. Plants were cultured for 22 days under the condition of $21{\pm}2^{\circ}C$, $60{\pm}10%$ RH, and $400{\pm}50{\mu}mol{\cdot}mol^{-1}\;CO_2$. The greatest leaf length and width, fresh and dry weights, and total anthocyanin content were obtained in the 24/0 photoperiod, regardless of the light intensity. Length of the longest root, fresh and dry weights, and number of leaves were greater in light intensity of $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ than 100 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Chlorophyll value was the greatest in the photoperiod 12/12 than 18/6 or 24/0. The results obtained suggest that plant grew the best kept by light intensity at 200 or $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and photoperiod of 12/12 or 18/6.

• Environmental Analysis Health and Toxicology
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• v.15 no.4
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• pp.123-130
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• 2000

2-Bromopropane, important industrial chemical, specially in electronic industry at Yangsan in Korea has been reported to cause amenorrhea for female and azoospermia, oligozoospermia or reduced sperm motility for male. 2-BP was investigated through 21 days of repeated dose in male Sprague-Dawley rats. The dose levels per body weight were 0 (control), 250,500 and 1,000 mg/kg. 2-BP dissolved in vehicle olive oil was injected into the intraperitoneum 6 times per week for 3 weeks, but 1,000 mg/kg dose group was 2 weeks because of serious illness. Male rats showed significant decreases in body weight and right and left testis showed typical weight losses depending on the 2-BP. The number of white blood cell and red blood cell , percentage of monocytes, and hemoglobin decreased significantly in high dose (P< 0.05). Red cell volume distribution width increased significantly in the high dose (P< 0.05). Histopathological findings of testes showed a decrease of spermatogenic cells, exfoliation of spermatid and spermatocyte, vacuolization of Sertoli cells and hyperplasia of Leydig cells. Protein band density between 113,000 dalton ($\beta$-galactosidase) and 53,900 dalton (ovalbumin) has decreased in 250 mg/kg dose group, but it has gradually increased to the higher density in 1,000 mg/kg dose group than in control group.

• Journal of Conservation Science
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• v.32 no.3
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• pp.395-402
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• 2016

The impact of a large water control project on surrounding environment(temperature and relative humidity, precipitation, wind speed, present weather and visibility) was monitored. The survey have targeted on Silleuksa temple which is located in the waterside. The number of foggy days emerged as an environmental factor that can cause damage to cultural heritage. Under construction of weir since 2011, monthly relative humidity of Yeoju and Icheon was dropped to 9.6%. It depends on decrease in the number of raining days and precipitation of each year. Silleuksa temple is contiguous to Namhan River and only 100 m away from the waterside. Average wind speed is 0.5 m/s. Silleuksa has a site environmental factors which is often foggy. The number of foggy days of Silleuksa temple declined to 53 days soon after weir's completion. In case of fog, relative humidity of outside was higher than that of outside and inside of Josadang Shrine. Relative humidity difference of outside and inside of Josadang Shrine was 5.4% on average. Relative humidity of the number of foggy days is 6.3% higher on average than that of fine days. The width and dimensions of Namhan River increased by 1.45 times after weir's construction. It can change the number of foggy days. A long-term monitoring is positively necessary on fog occurrence and relative humidity.