• Journal of Veterinary Science
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• 제21권2호
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• pp.31.1-31.5
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• 2020

In this study, whiteleg shrimp (Penaeus vannamei) imported from Vietnam were collected from South Korean markets, and examined for 2 viruses: infectious hypodermal and hematopoietic necrosis virus (IHHNV, recently classified as decapod penstyldensovirus-1), and white spot syndrome virus (WSSV). Among 58 samples, we detected IHHNV in 23 samples and WSSV in 2 samples, using polymerase chain reaction and sequencing analyses. This is the first report of IHHNV and WSSV detection in imported shrimp, suggesting that greater awareness and stricter quarantine policies regarding viruses infecting shrimp imported to South Korea are required.

• 한국어병학회지
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• 제35권1호
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• pp.129-133
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• 2022

The full genome sequence of a Korean white spot syndrome virus (WSSV, isolate: WSSV-GoC18) is presented here. We obtained a total of 12,320,554 reads with 291,172 bases, 170 gene, and 170 coding DNA sequence, which were assembled in 1 contig. Phylogenetic analysis revealed that the WSSV-GoC18 was closely related to Chinese isolate (WSSV-PC) and distinctly different with previously reported a Korean isolate (WSSV K-LV1). The complete genome sequence of WSSV isolates will be of great help in molecular epidemiological studies, contributing to molecular diagnosis and disease prevention in shrimp aquaculture.

• Journal of Microbiology and Biotechnology
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• 제17권3호
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• pp.511-515
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• 2007

White-spot syndrome virus (WSSV) is a devastating, infectious virus affecting shrimp. Although sensitive techniques involving PCR have been developed to assist farmers in screening shrimp (brood stock) for WSSV prior to stocking ponds, such practices have not yet been applied in Korea. Despite the rationality of implementing screening, there has been some doubt as to whether the stocking of WSSV-PCR-negative fly epidemiologically decreases white-spot disease outbreaks. Here, we report a retrospective analysis of data from shrimp farms in the western coast of Korea where WSSV-PCR-negative brood stocks were used to stock rearing ponds. A total of 366 shrimp from Heuksan Island were sampled for WSSV with PCR. Of the tested shrimp, 7.2% (28 brood stocks) were identified as WSSV positive; only WSSV-PCR-negative shrimp were used for brood stocks. Total unit production (final shrimp production/ the area of the ponds) was higher, at 1.96, in ponds where WSSV-PCR-negative shrimp were used, as compared with 1.02 in other ponds in Korea in 2004. This retrospective analysis of WSSV in Korea may be useful to the shrimp aquaculture industry, suggesting a testable hypothesis that may contribute to the eventual control of WSSV outbreaks.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1477-1482
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• 2001

Research in low cost feeds with high nutritional value and immunogenicity is important to reduce production cost and increase yields in the shrimp industry. In this study, immunostimulants of bacterial origin (peptidoglycan and lipopolysaccharides) and egg white were incorporated in shrimp diets as feed additives to determine the growth, survival and tolerance of Penaeus monodon to white spot syndrome virus (WSSV). Although the results obtained were not statistically significant (p>0.05) among the treatments, shrimp fed with bacterial additives and egg white showed higher weight gain, specific growth rate and survival than those fed on commercial shrimp diet. Shrimp fed with artificial diet showed 100% mortality when challenged with WSSV. However, shrimp fed on peptidoglycan supplemented diet had higher survival than their counterpart, whereas shrimp fed on egg white supplemented diet had a higher specific growth rate and better tolerance when challenged with WSSV. Further studies are required to determine the effectiveness and optimization of bacterial strains and egg white as feed additives to increase production and enhance the shrimp immune response to diseases.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2001년도 추계 한국양식학회 학술대회
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• pp.227.2-228
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• 2001

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.519-520
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• 2001

1993년 초여름부터 발생한 양식대하의 WSBV에 의한 대량 폐사가 1996년에는 폐사율이 거의 75％에 이를 정도로 피해가 많이 발생하였다(허 등, 1998). WSBV에 감염된 새우는 두흉갑과 체표에 흰반점 증상이 나타나므로 병명을 일반적으로 White spot syndrome disease(WSSD)라고 하고 원인 바이러스를 WSBV라 일반적으로 명명하고 있고 이와 같은 증상으로 인한 새우의 대량 폐사는 아시아 전역에서 일어나고 있다(Chou 등, 1995; Inouye 등, 1996). (중략)

• 환경생물
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• 제35권4호
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• pp.461-467
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• 2017

Shrimps infected with WSSV(White Spot Syndrome Virus) generally exhibit white spots in their inner space of carapaces as an acute clinical sign. In an effort to identify the correlation between this acute clinical sign and the condition, the index factors (RNA/DNA concentration and ratio, trypsin activity) were analyzed. A total 580 farmed Fenneropenaeus chinensis and 130 Lithopenaeus vannamei were collected from western and southern fifteen outdoor ponds in Korea. The status of the white spot pathology was divided into four stages (stage 0, stage I, stage II, and stage III), in accordance with the clinical signs as to the size and area of white spots. A significant decrease in RNA concentration and RNA/DNA ratio for multi-infected fleshy prawn (WSSV and vibrio sp.) occurred during the stage III (the whole carapace is covered with a white spot). In particular, RNA/DNA ratio was significantly lower as $1.47{\pm}0.04$ than other groups. A similar trend was also found in the single infection (WSSV), but the decrease was less than the multi-infection. In the species comparison, both species were vulnerable to the multi-infection, but L. vannamei was more sensitive than F. chinensis(ANOVA, p<0.05): A significant decrease in RNA concentration and RNA/DNA ratio was first found in stage II for the former species, while it was found in stage III for the latter species. Trypsin activity was also showed a similar tendency with nucleic acid variation. Multi-infected shrimp showed drastically decrease of trypsin activity. According to the results, clinical signs of the white spot under carapace have an only physiological effect on shrimp if they covered entirely with white spots.

• 한국어병학회지
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• 제17권3호
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• pp.213-216
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• 2004

To confirm the possibility of a wild marine crab, Gaetice depressus, as a carrier for white spot syndrome virus (WSSV) and to develop an alternative experimental model for WSSV in winter season, the susceptibility of the crab to WSSV was assessed by artificial challenge and subsequently tested for infection by PCR assay. The results revealed that the crabs were as highly susceptible as penaeid shrimps. WSSV caused 100% mortality in G. depressus within 16 days after intramuscular injection. The presence of WSSV in the moribund crabs was confirmed by PCR and was found in gills and muscle tissue. These results suggest that G. depressus can be naturally infected by WSSV via moribund shrimps, and can act as a potential carrier of WSSV. In addition, G. depressus can be used as an alternative experimental animal for WSSV.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2001년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.521-523
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• 2001

White spot syndrome virus (WSSV) causes the most serious epizootic in cultured penaeid shrimp. The epizootic started in 1992, and spread through east and south east Asia and into other shrimp growing countries of the region. WSSV has also been reported from Penaeus chinensis cultured in Korean peninsula (Park et al., 1998; Kim et al., 1999). However, this paper deals with the WSSV infection in cultured and wild P. chinensis, purification and characterization of the pathogen, and the occurrence of other concomitant infection. (omitted)

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2005년도 국제학술심포지움 The 44th Annual Meeting of Korean Society for Life Science
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• pp.92-92
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• 2005