• Journal of Microbiology and Biotechnology
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• 제10권2호
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• pp.215-220
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• 2000

Abstract White spot disease (WSD), resulting in more than 90% mortality of aquacultured penaeid shrimp, has been reported off the southern and western coasts of Korea since 1993. The pafuogen of WSD has been identified as being a virion wifu an envelope around a central nucleocapsid, and with an average size of 167 nm in diameter and 375 nm in length. In the present study, an in situ hybridization technique was developed as a rapid. sensitive, and specific diagnostic assay for the WSD viros infection in shrimp. Furthermore. the pathological changes ofWSD, in shrimp experimentally infected with WSD viroses. were investigated. Using a biotinylated 643 bp probe obtained from a peR using primers specific to the rod-shaped virus of Penaeus japonicus (RV-PJ), positive signals were detected in both naturally and experimentally infected shrimps. The in situ hybridization revealed positive reactions in the nuclei of the stromal matrix cells in the lymphoid organ, epithelia of the gills, foregut. epidermis, and hematopoietic cells of the interstitial tissues, suggesting the presence of WSD virus. Tills result indicates that the in situ hybridization method can be useful for a rapid and sensitive detection of WSD viruses in shrimp.shrimp.

• Journal of Microbiology and Biotechnology
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• 제11권3호
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• pp.394-398
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• 2001

Because of the great concern over the possibility of contamination from the rod-shaped nuclear virus (PRDV) from Japan and white spot virus (WSSV) from Taiwan, most eggs used in Korean shrimp farms are currently obtained from local broodstock. In addition, the screening of imported broodstock for any viral presence at the National Fisheries Research and Development Institute is also mandatory. Nonetheless, massive mortality from white spot syndrome continues in Korea. In the present study, we present an improved PCR method to use tissue-extracted DNA instead of viral DNA extracted from a purified virus based on a sucrose density gradient, and produced results within 8 h. In 1998, this modified PCR method was able to detect that diseased Penaeus japonicus were infected within 8 h. In 1998, this modified PCR method was able to detect that diseased Penaeus japonicus were infected only with PRDV, while Fenneropenaeus chinensis were infected with both PRDV and WSSV. In 1999, PRDV and WSSV were detected in F. chinensis with signs of infection, but not with WSSV alone.

• BMB Reports
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• 제37권6호
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• pp.726-734
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• 2004

White spot disease (WSD) is caused by the white spot syndrome virus (WSSV), which results in devastating losses to the shrimp farming industry around the world. However, the mechanism of virus entry and spread into the shrimp cells is unknown. A binding assay in vitro demonstrated VP28-EGFP (envelope protein VP28 fused with enhanced green fluorescence protein) binding to shrimp cells. This provides direct evidence that VP28-EGFP can bind to shrimp cells at pH 6.0 within 0.5 h. However, the protein was observed to enter the cytoplasm 3 h post-adsorption. Meanwhile, the plaque inhibition test showed that the polyclonal antibody against VP28 (a major envelope protein of WSSV) could neutralize the WSSV and block an infection with the virus. The result of competition ELISA further confirmed that the envelope protein VP28 could compete with WSSV to bind to shrimp cells. Overall, VP28 of the WSSV can bind to shrimp cells as an attachment protein, and can help the virus enter the cytoplasm.

• 한국수산과학회지
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• 제51권1호
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• pp.47-53
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• 2018

The presence of pathogens in wild fish is a potential threat to the fish being raised nearby fish farm. Surveillance of these pathogens in the wild is, thus, highly important to keep fish in the farm safe from serious communicable diseases. Fish and crustacean were sampled 4 times at Soyang Lake in 2016. Pathogens for five reportable communicable diseases including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease), and parasites were investigated. In Soyang Lake, pale chub Zacco platypus was the most abundant. Forty seven pale chub, 8 Korea piscivorous chub Opsarichthys uncirostris, 3 oily shiner Sarcocheilichthys variegatus wakiyae and 2 crucian carp Cyprinus carassius were investigated for detection of target diseases of fish. Seventeen Caridina denticulata denticulata, 12 Oriental river prawn Macrobrachium nipponense and 1 Chinese mitten crab Eriocheir sinensis were used for detection of WSD. As the result, reportable communicable diseases were not detected in all the freshwater animals. Dactylogyrus was detected in Korea piscivorous chub. Copepoda was also detected in pale chub and Korea piscivorous chub. Metacercaria was detected in the pale chub sampled in September and October. Those were not metacercaria of liver fluke Clonorchis sinensis.

• 환경생물
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• 제37권4호
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• pp.474-482
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• 2019

자연수계 수산생물의 전염병 모니터링은 자연수계 및 양식 수산생물의 질병 관련성 및 상관관계 구명은 질병 발생 예방 및 확산 방지에 매우 중요한 역할을 한다. 2018년 해구 64개소에서 어류 39종 977마리 및 갑각류 14종 287마리를 선정하여 총 1,264마리에 대하여 병원체 감염 여부를 조사하였다. 어류는 법정전염병 2종(VHS, RSIVD) 및 비법정전염병 3종(MABVD, HRVD, LCD)을 분석하고 갑각류는 법정전염병 6종(WSD, IHHN, TS, IMN, YHD, WTD)을 분석하였다. 조사한 모든 시료에서 전염병이 검출되지 않았지만, 우리나라에서 조사한 자연수계 수산생물이 무병하여 청정국 또는 청정지위 선언에 중요한 정보를 제공할 것이다.

• 한국수산과학회지
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• 제51권3호
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• pp.248-253
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• 2018

Freshwater farms are primarily located adjacent to rivers and lakes, facilitating the introduction and spread of pathogens into natural systems. Therefore, it is necessary to continuously monitor natural aquatic organisms, the breeding environment, and infection rates by pathogenic organisms. Fish and crustaceans were sampled 4 times in the Geum River estuary in 2016. The samples were analyzed for the presence of pathogens for reportable communicable diseases, including KHVD (koi herpesvirus disease), SVC (spring viraemia of carp), EUS (epizootic ulcerative syndrome) and WSD (white spot disease); parasite abundance was also examined. The dominant fish species were deep body bitterling Acanthorhodes macropterus (21.4%), followed by skygager Erythroculter erythropterus (12.7%). For crustaceans, Palaemon paucidens and Chinese mitten crab Eriocheir sinensis were dominant. Sixty fish and 36 crustacean species were examined for reportable communicable diseases. When using a specific primer set for each disease, PCR analysis did not detect any reportable communicable diseases in the samples. Some instances of Dactylogyrus, copepods, nematodes and metacercaria were detected. However, the PCR results indicated that the metacercaria were not Clonorchis sinensis.

• 생태와환경
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• 제52권4호
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• pp.306-315
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• 2019

한강 하구는 오염물질과 병원성 미생물의 유입 및 확산 가능성으로 인해 이곳에 서식하는 수산생물에 피해가 발생할 수 있다. 하지만 지금까지 이 지역 서식 생물에 대한 법정전염병 병원체 감염 여부가 조사된 자료는 찾아보기 힘들다. 본 연구에서는 한강 하구 전류리에 서식하는 수산 생물 중 잉어과 어류와 갑각류를 대상으로 법정전염병(잉어봄바이러스병, SVC; 잉어허피스바이러스병, KHVD; 유행성궤양증후군, EUS; 흰반점병, WSD) 및 기생충의 감염 여부를 조사하였다. 전류리 유역에서 채집된 어류는 잉어과 어류가 가장 많았으며 주연성 어류와 일차 담수어가 같이 관찰되었고, 갑각류는 해산종이 채집되었다. 채집된 잉어과 어류와 갑각류에 대해 PCR을 이용한 법정전염병 검사 결과 이들 질병에 감염된 개체는 없었다. 기생충성 질병 검사 결과 공중보건상 위협요인인 간흡충이 검출되지는 않았지만 일부 어류에서 다른 흡충류의 metacercaria와 nematode, cestode, copepod, 단생충 및 구두충 등의 감염을 확인할 수 있었다. 이상의 결과는 아직 한강 하구가 주요 수산생물 전염병 병원체에 심각하게 오염되지 않았음을 의미한다. 그러나 한강의 수질 상태는 지속적으로 변화하고 수서 생태계에 대한 전염병의 파괴력은 예측하기 힘들 정도로 위험하므로, 지속적인 방역 노력이 진행되어야 할 것이다. 이를 위해 조사 지역을 전류리뿐만 아니라 이곳보다 상류로 확대하고 조사 개체 수를 늘려서 질병모니터링을 실시할 필요성이 제기된다.