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Stage specific transcriptome analysis of liver tissue from a crossbred Korean Native Pig (KNP × Yorkshire)

  • Kumar, Himansu;Srikanth, Krishnamoorthy;Park, Woncheol;Lee, Kyung-Tai;Choi, Bong-Hwan;Kim, Jun-Mo;Lim, Dajeong;Park, Jong-Eun
    • Journal of Biomedical and Translational Research
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    • v.19 no.4
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    • pp.116-124
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    • 2018
  • Korean Native Pig (KNP) has a uniform black coat color, excellent meat quality, white colored fat, solid fat structure and good marbling. However, its growth performance is low, while the western origin Yorkshire pig has high growth performance. To take advantage of the unique performance of the two pig breeds, we raised crossbreeds (KNP ${\times}$ Yorkshire to make use of the heterotic effect. We then analyzed the liver transcriptome as it plays an important role in fat metabolism. We sampled at two stages: 10 weeks and at 26 weeks. The stages were chosen to correspond to the change in feeding system. A total of 16 pigs (8 from each stage) were sampled and RNA sequencing was performed. The reads were mapped to the reference genome and differential expression analysis was performed with edgeR package. A total of 324 genes were found to be significantly differentially expressed (${\left|log2FC\right|}$ > 1 & q < 0.01), out of which 180 genes were up-regulated and 144 genes were down-regulated. Principal Component Analysis (PCA) showed that the samples clustered according to stages. Functional annotation of significant DEGs (differentially expressed genes) showed that GO terms such as DNA replication, cell division, protein phosphorylation, regulation of signal transduction by p53 class mediator, ribosome, focal adhesion, DNA helicase activity, protein kinase activity etc. were enriched. KEGG pathway analysis showed that the DEGs functioned in cell cycle, Ras signaling pathway, p53 signaling pathway, MAPK signaling pathway etc. Twenty-nine transcripts were also part of the DEGs, these were predominantly Cys2His2-like fold group (C2H2) family of zinc fingers. A protein-protein interaction (PPI) network analysis showed that there were three highly interconnected clusters, suggesting an enrichment of genes with similar biological function. This study presents the first report of liver tissue specific gene regulation in a cross-bred Korean pig.

Comparison Study on the Make-up Cultures between the Ching dynasty in China and the Edo Age in Japan based on their Aesthetic Consciousness (중국(中國) 청(淸) 왕조시대(王朝時代)와 일본(日本) 강호시대(江戶時代)의 미의식(美意識)에 따른 화장문화(化粧文化) 비교 연구)

  • Ahn, Hyun-soon;Koh, Jung-min
    • Cross-Cultural Studies
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    • v.27
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    • pp.59-79
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    • 2012
  • China and Japan are geographically close and the two countries had shared the Chinese Character Culture and the thoughts of Confucianism, Buddhism and Taoism since the ancient age. They also actively exchanged culture in various areas. Some cultural exchanges had been caused by surrounding environment and culture had been introduced to other country in a natural way; while some cultural exchanges had been forcibly introduced through artificial process. It is believed that such cultural phenomenon must have had impact on the make-up cultures of the two countries and it was assumed that there must have been commons and differences in the make-up cultures of the two countries. This study explored the historical background of the Ching dynasty of China and the Edo Age in Japan, which are in the same time frame, and studied the aesthetic consciousness of the two countries at the time. Then the make-up style of ladies in the two countries had been studied to find out how their aesthetic consciousnesses had been expressed in the make-ups of the two countries. Then the commons and differences in make-up skills between the two countries had been identified. According to the study results, the main stream of aesthetics during the Ching dynasty in China can be classified into Confucianism aesthetics and Taoism aesthetics. On the other hand, the main stream of aesthetics during the Edo Age in Japan can be classified into "mitate(見立)", "ikki(いき)" and "garumi(かるみ). The skin care in the make-up culture of Ching dynasty in China was based on "rouge (?脂, yanzhi)" and "powder(粉, fen)". The Ching ladies loved the make-up style using rouge. It had been same both in the high society and common people. The eyebrow care was delicate and curved so that the feminine beauty with elegant spirit could be emphasized. The lips had been expressed to be smaller and the ladies tried to express elegance and reliability, rather than frail and tender feminine image. The skin care in the make-up culture of Edo Age in Japan focused on even applying of white powder so that the face would look soft. The eyebrow make-up was a very important part of the make-up. The shapes of eyebrow had been advanced in various styles and there had been eyebrow make-up styles such as "crescent-shaped brow (三日月), "crane style brow (鶴眉) and "Tang style brow (唐眉). The lips had been applied of thick red color, imitating the make-up skill of the ladies in the entertainment business. The lips make-up skill expressing the lips in two colors had been quite popular. Among the make-up skills during the Edo Age in Japan, the "black teeth (齒黑)" can be said as the most unique make-up style of Edo Age.

Selection of Primula malacoides Lines with Different Growth and Flowering Characteristics (생장이 다양한 Primula malacoides 순계 선발)

  • Song, Cheon Young;Lee, Jeong Ho
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.3
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    • pp.152-157
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    • 2009
  • To obtain pure lines for breeding $F_1$ varieties of Primula malacoides, 10 lines that were considered uniform in growth and flowering characteristics were selected from self-crossings of the sixth generation ($S_6$). Plant height of the selected lines ranged from 20.8 to 33.2 cm, and number of leaves ranged from 33.6 to 54.2. The selected lines flowered at 181.4 to 207.8 days after sowing. Three lines, including 'PR-03-81', flowered in less than 190 days from sowing, while six lines, including 'PR-03-228', only flowered after more than 200 days from sowing. In terms of petal color, six lines, including 'PR-03-81', were pink, three, including 'PR-03-236', were purple, and line 'PR-03-275' was white. Flower diameter ranged from 2.4 to 2.9 cm, and number of pedicels per plant was between 15.4 and 26.4. Number of flowers per pedicel ranged from 20.2 to 30.2. Germination was more than 70%, and number of seeds per capsule (pod) ranged from 110.0 to 162.8. In summary, plant height was low and flower diameter was small and plants had many flowers and readily formed seeds. The selected lines are not only valuable as ornamental plants but could also be used as breeding materials for $F_1$ hybrids of Primula polyantha.

Investigation of Plant Injury under Ambient Air Pollutants (대기오염물질에 의한 농작물 피해원인 조사)

  • Lee, Jong-Sik;Shin, Joung-Du;Kim, Jin-Ho;Jung, Goo-Bok;Kim, Won-Il;Kim, Gun-Yeob
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.1
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    • pp.31-35
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    • 2007
  • In order to find out the cause of plant injury, the symptom of plant injury, and contents of element concerned in the plant were analysed. Also, a case study was conducted to find out the factor of plant injury at a agriculture and industry complex in Gyeongsang province in 2004. The distribution of isomeric curve was made with meteorological data, toxic gas concentration exhausted from pollution source. The general symptom of plant injury by ammonia gas was dry and dead of leaves with white color. At low concentration of ammonia gas, plant leaf showed spots of reddish brown. The characteristic of plant injury symptom by hydrogen fluoride gas was that the symptom was appeared at the edge of leaf. The isomeric curve of sulfur dioxide at the region, where the plant was damaged, showed that the area was affected by exhausted gas from the pollution source. Especially, this area was affected more deeply at summer than any other season.

Nutrition and Sensory Properties of Kimchi prepared with Dioscorea oppasita (마(Dioscorea opposita)김치의 영양성분 및 관능적 특성)

  • Yang, Kyung-Mi;Kong, Hyun-Joo;Kwon, Ji-Eun
    • Journal of Digital Convergence
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    • v.19 no.7
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    • pp.379-390
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    • 2021
  • In this study, we produced kimchi with cooked Dioscorea batatas yam. Sliced kimchi cabbage, processed white radish kimchi (kkakdugi), and whole kimchi cabbage were prepared with 3% Dioscorea batatas, the nutritional and sensory properties were evaluated. The three types of D. opposita kimchi showed energy content in the range of 35-40 kcal per 100 g, and no crude fat, saturated fat, trans fat, or cholesterol was detected. Sodium content was 450-650 mg, with sliced cabbage kimchi showing the highest concentration. The vitamin C content increased significantly after 28 days of storage, with the sliced cabbage kimchi and whole-cabbage kimchi showing larger increases than the radish kimchi. All three types of D. opposita kimchi showed high Ca, K, and Mg contents. The whole-cabbage kimchi maintained salinity at a level of 2.38-2.72% from immediately after preparation to 28 days of storage compared to sliced cabbage kimchi and radish kimchi. Sliced cabbage and whole kimchi cabbage kimchi showed an increase in lightness and a decrease in redness with longer storage duration. Compared to the control groups, the experimental groups (with added D. opposita) showed higher yellowness. The hardness of the three types of kimchi decreased with longer storage duration. In sensory evaluation, there were no significant differences between the control groups and the experimental groups.

A New Early Flowering, Spray Chrysanthemum Cultivar for Cut Flower, "Pinky" with Single Type and Pink Petals (조기개화성의 절화용 핑크색 흩꽃 스프레이국화 "핑키" 육성)

  • Hwang, Ju-Chean;Chin, Young-Don;Chung, Yong-Mo;Kim, Su-Gyeong
    • Korean Journal of Breeding Science
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    • v.40 no.2
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    • pp.196-200
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    • 2008
  • A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Pinky' was bred from a cross between 'Rima' and 'Yen'. Progenies were investigated from 2004 to 2006 under shading culture in spring and retarding culture in autumn and the promising line was selected based on the performance of year-round production, white rust resistance and vase life. Pinky flowered around 20 October, and year-round production was possible by shading and lighting. Its flower was 3.1 cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. Days to flower from short-day start for 'Pinky' was about 47 in spring. 'Pinky' showed a vase life of 20.9 days in autumn. This cultivar was commercially registered in 2007. 'Pinky' would be suitable for culture under the greenhouse and rain shelter condition in Korea.

Quality Characteristics of Muffin Added with Red Yeast Rice and white rice (홍국 첨가량에 따른 머핀의 품질특성)

  • Choi, Hyun-Sook;Nam, Hae-Young
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1442-1455
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    • 2018
  • The objective of this study was to examinate the differences of general component, chromaticity, pH, weight, loss on baking, specific volume bulk, diameter of upper side and height, mechanical texture, DPPH radical, ABTS radical, Total amount of phenol, Monacolin-K content, sensory test on Muffin using no Red Yeast Rice and different levels (2%, 4%, 6%, 8%, 10%) of Red Yeast Rice. pH was decreased as the amount of added Red Yeast Rice increased(control 8.35, RM2% 7.93, RM4% 7.86, RM6% 7.69, RM8% 7.49, RM10% 7.30). Weight and loss on baking were showed not too different depending the amount of added Red Yeast Rice, and the baking loss were at very low point. Adhesion, cohesiveness, elasticity, viscosity, and chewiness showed no attentive differences on mechanical texture analysis. As the level of Red Yeast Rice increased, DPPH radical, ABTS radical, and Total phenol content decreased. Monacolin K were at higher point as the level of Red Yeast Rice increased. The sensory test showed Taste, Texture, and Overall acceptability gained the highest point when using 4% of Red Yeast Rice. On the sensory test, 4% of Red Yeast Rice was considered to be the best level based on Flavor and Color. The results of these tests show 4% of Red Yeast Rice is most preferred and considered to be most suited with flour and rice flour.

Morphological Development of Egg and Larvae of Gnathopogon strigatus (Gobioninae) (줄몰개(Gnathopogon strigatus)의 난 발생 및 자치어 형태 발달)

  • Kim, Kyeong-Mu;Jeon, Hyoung-Joo;Song, Ha-Yun
    • Korean Journal of Ichthyology
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    • v.31 no.3
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    • pp.131-140
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    • 2019
  • This study is to observe species identification and early life history of Gnathopogon strigatus and to use it as a basis for taxonomic studies and conservation of species. For the experiments, the mature adults were collected at the Wang-suk Stream located in Namyang-ju city, Gyeong-gi Province and eggs were artificially fertilized by the wet method in the laboratory. The shape of the fertilized egg was globular, adhesive, opaque white in color and had no oil globules. The fertilized egg was 1.66~1.88 mm (average 1.76 mm, n=30) in diameter. The blastular stage occurred at 3 hours 05 minutes after fertilization and the gastrular stage was detected at 8 hours 30 minutes after fertilization. The embryo began to hatch about 54 hrs after fertilization under water temperature of $23{\pm}1^{\circ}C$ and the newly hatched larva (yolksac larva) were 4.1~4.7 mm (mean 4.4 mm, n=20) in total length (TL). The fourth day after hatching, the postlarva were 5.4~5.9 mm (mean 5.6 mm, n=20) in total length, their york sacs were completely absorbed and Start eating Artemia sp. Ten days after hatching, flexion larva were begins Notochord flexion were 7.5~8.6 mm (mean 8.1 mm, n=20) in total length. Sixteenth day after hatching, postflexion larva were complete Notochord flexion were 8.2~9.7 mm (mean 9.1 mm, n=20) in total length. At thirty-eight days after hatching, Juvenile were arrive integer all fin rays and similar to those of adults were 11.3~15.5 mm (mean 13.3 mm, n=20) in total length.

A Preliminary Study on the Attractiveness of Yellow Sticky Trap for Insect Pests According to the Installation Angle of Traps in Strawberry Farms (시설딸기 농장에서 황색점착트랩 설치각도에 따른 주요 해충의 유살특성 예비연구)

  • Kim, Sanghee;Kim, Subin;Kim, Dong-Soon
    • Korean journal of applied entomology
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    • v.58 no.2
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    • pp.143-149
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    • 2019
  • This study was conducted to examine the attractiveness of yellow sticky trap (YST) for insect pests by the angle of inclination of the trap surface. In strawberry farms with high bed system, YSTs were installed to attract insect pests in vertical direction with one surface, horizontal direction with upper and under surface, and angle of $45^{\circ}$ with upper and lower sticky surface. Thrips (Frankliniella occidentalis in dominance) and Sciaridae (Bradysia agrestis in dominance) species were more attracted on upper surface of $45^{\circ}$ angle trap, vertical surface and upper surface of horizontal trap than on lower surface of $45^{\circ}$ angle trap or horizontal trap. Cicadellidae (Empoasca vitis in dominance) species were caught more on vertical surface and upper surface of $45^{\circ}$ angle trap than on other traps. There were no specific trend in capture of aphid (Aphis gossypii in dominance) and white fly (Trialeurodes packardi in dominance) species among traps, probably because of a low density of the pest species.

'Jungmo2509', the First Rye Cultivar of Self-Fertility in the Korea (국내 최초의 자식성 호밀 품종 '중모2509')

  • Han, Ouk-Kyu;Kim, Jin-Jin;Kim, Dea-Wook;Ku, Ja-Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.41 no.2
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    • pp.77-83
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    • 2021
  • Rye (Secale cereal L.) is the most tolerant to abiotic stress including low temperature, drought, and unfavorable soil conditions among the winter cereals. Rye is the rapid growth of early spring results from increasing areas for the use of the forage and green manure in the middle part of Korea. "Jungmo2509", a rye cultivar was developed by the National Institute of Crop Science (NICS), RDA in 2014. It was developed from a cross between "Olhomil", a self-compatible cultivar, and "Synthetic II", a self-incompatible line. "Jungmo2509" is an erect plant type and of a middle size, with a green leaf color, a yellowish-white colored culm, and a yellowish brown-colored, small-size grain. The heading date of "Jungmo2509" was April 23, which was 5 days later than that of "Gogu", respectively. But "Jungmo2509" showed greater resistance to lodging compared to that of the check cultivar, with similar to winter hardiness, wet injury, and disease resistance. "Jungmo2509" was a higher to than "Gogu" in terms of protein content (9.4% and 8.0%, respectively), total digestible nutrients (TDN) (55.7% and 55%, respectively). The seed productivity of "Jungmo2509" was approximately 2.08 ton 10a-1, which was 11% lower than that of the check. Almost all rye cultivars are out-crossing due to genes controlling incompatibility, but "Jungmo2509" is higher seed fertility (56%) than that of Gogu (0%). it has self-compatible genes. "Jungmo2509" is erect in plant type and resistance for lodging. Therefore, "Jungmo2509" can produce uniform seeds for processed grains of human consumption and utilize them as parents for breeding the rye hybrids with high forage yields.