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Growth Stimulation of Lactic Acid Bacteria by a Radish Component (무의 젖산균 증식촉진물질과 촉진작용)

  • Park, Kyung-Suk;Kyung, Kyu-Hang
    • Korean Journal of Food Science and Technology
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    • v.24 no.6
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    • pp.528-534
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    • 1992
  • Growth stimulatory material for lactic acid bacteria was extracted from radish and radish green juice and its growth stimulatory effect was tested. Dried methanol-precipitated growth stimulatory material was lightly grayish white powder, Its ash content is 44% and approximately 50% of the ash is sulfur. It has reddish brown color upon solubilization in water. The material had unchanged stimulatory effect when it was treated with proteinase or pectinase, or ashed. The growth stimulatory activity was dialyzable. The material was able to counteract the growth inhibitory effect of EDTA. When selected lactic acid bacteria were grown at $30^{\circ}C$ for 24 hours in peptone(0.5%)-yeast extract(0.5%)-glucose(2%) broth with and without 0.5% growth stimulatory material, the material stimulated the growth of Lactobacillus plantarum, L. fermentum, L. leichmanii, L. sake, L. brevis, L. acidophilus, L. casei, Pediococcus pentosaceus, Leuconostoc mesenteroides, Streptococcus faecalis, S. lactis, S. cremoris and S. thermophilus by 19, 1833, 133, 444, 840, 32, 14, 18, 6, 17, 4, 5 and 4 times, respectively.

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A Thermostable Protease Produced from Bacillus sp. JE 375 Isolated from Korean Soil (한국의 토양으로부터 내열성 단백질 분해효소를 생산하는 Bacillus sp. JE 375의 선별)

  • Kim, Ji-Eun;Bai, Dong-Hoon
    • Korean Journal of Food Science and Technology
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    • v.38 no.3
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    • pp.419-426
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    • 2006
  • A thermophilic microorganism, strain JE 375, which produces a thermostable protease, was isolated from soil and compost in Korea. This gram-positive, rod-shaped, catalase positive, motility positive, and hemolysis ${\beta}$ containing organism was implicated in glucose fermentation, mannitol fermentation, xylose oxidation, aerobic activity and spore formation. The color of the colony was yellowish white. The temperature range for growth at pH 6.5 was between 55 and $70^{\circ}C$, with an optimum growth temperature of $65^{\circ}C$. This result confirmed the strain JE 375 as a thermophilic microorganism. The enzyme was produced aerobically at $65^{\circ}C$ during 20 hr in a medium (pH 6.5) containing 1% trypton. 1% maltose, 0.5% yeast extract and 1% NaCl. The 16S rDNA of strain JE 375 had 97.6% sequence similarity with the 16S rDNA of Bacillus caldoxyloyticus. On the basis of biochemical and physiological properties and phylogenetic analysis, we named the isolated strain as Bacillus sp. JE 375. The thermostable protease from Bacillus sp. JE 375 had been partially purified and characterized. The molecular weight of the enzyme was deduced from SDS-PAGE and gel chromatography as 55 kDa and its optimal temperature was $60^{\circ}C$. The enzyme showed its highest activity at pH 7.5 and was stable from pH 7.0 to 8.0.

Floral Characteristics of Asteraceae Flowers and Insect Pollinators in Korea (우리나라 국화과 식물의 화기구조와 방화 곤충 연구)

  • Kim, Gab-Tae;Lyu, Dong-Pyo;Kim, Hoi-Jin
    • Korean Journal of Environment and Ecology
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    • v.26 no.2
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    • pp.200-209
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    • 2012
  • To search for the co-relationships between insect-pollinators and the plant species of Asteraceae, insects visiting in flower and the flowers in Korea, were studied from April 2010 to October 2011. The sum of flower visiting degrees are shown 38 in Lepidoptera, 38 in Diptera, 36 in Hymenoptera, and the lowest 6 in Coleoptera, respectively. 65 insect species are identified pollinators, Hymenoptera 13 species(Apidae 11 sp., Formicidae 2 sp.), Lepidoptera 29 species(Pieridae 5sp., Nymphalidae 12 sp., Satyridae 3 sp., Hesperirdae 3 sp., Lycaenidae 2 sp., Danaidae 1 sp., Moth 2 sp.), Diptera 16 species(Tachinidae 1 sp., Syrphidae 12 sp., Muscidae 1sp., Others 2 sp.), and Coleoptera 6 species(Cetoniidae 1 sp., Cermbycidae 3 sp., Chrysomelidae 1 sp., Mordellidae 1 sp.). 31 pollinator species visits the flower of $Erigeron$ $annuus$, next 15 pollinator species does the flower of $Eupatorium$ $japonicum$, and then 13 pollinator species does the flower of $Aster$ $ageratoides$. Only 2 pollinator species visit the flower of $Tephroseris$ $kirilowii$, $Ixeridium$ $dentatum$, $Inula$ $britannica$ var. $japonica$, $Carduus$ $crispus$, $Ligularia$ $fischeri$, $Ainsliaea$ $acerifolia$, $Synurus$ $deltoides$, $Cirsium$ $setidens$, $Crepidiastrum$ $enticulatum$ and $Dendranthema$ $boreale$. Pollinators of Lepidoptera visit more frequently white flower than yellow or purple one. This study found out that mutualisic relations between plants and insect pollinators is carried out in Korea.

Yielding Ability and Characteristics of Collections of Local Sesame (Sesamum indicum L.) (참깨 지방수집종의 주요형질과 수량성에 관한 연구)

  • 김규진;이정일
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.26 no.3
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    • pp.263-268
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    • 1981
  • Fourteen hundred and one landraces of sesame cultivated in Korea were collected and evaluated for their major agronomic characters and yield potential under conditions of May 15 sowing, 50\timesl5cm planting density and 4-3-2 kg/l0a of N-P_2 $O_{5}$-K_2 O application in Suweon in 1977. Seventy nine percent of the collection had white seed coat color, and 29% was brown. One capsule type with two rooms-four cells was 75%, one capsule type with four rooms-eight cells was 20%, and three capsule type with two rooms-four cel1s were 5% of the varieties collected, But, two one-capsule type varieties with three rooms-six cells were also found. Days to flowering from emergence were long in the varieties collected in the central northern area, but short in the varieties collected in the southern area. Number of capsule per plant was more in the central northern varieties than in the southern varieties. Number of branch per plant was more in the one capsule type varieties with two rooms-four cells than in the three capsule type varieties with two rooms-four cells and in the one capsule type varieties with four rooms-eight cells. There were significant corelations between number of capsules per plant and grain yields in the one capsule type varieties with four cells. Grain yields had significant correlation with number of capsules per plant and number of branches per plant.

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Germination and Early Growth Characteristics of the Halophyte Suaeda Asparagoides Under Various Environmental Conditions (염생식물 나문재의 여러 가지 환경 조건 하에서의 종자 발아 및 초기생장특성)

  • Kwon, Hyeok-Gyu;Jeong, Jae-Hyeok;Lee, Seung Jae;Chung, Nam-Jin
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.61 no.3
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    • pp.222-226
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    • 2016
  • This research was carried out to investigate the germination and seedling growth characteristics of the halophyte Suaeda asparagoides under various salt concentrations, temperatures, and the presence or absence of light S. asparagoides has a non-endospermic dicotyledonous seed structure that is go stone-shaped with a dark brown color and covered by a star-shaped perianth. Germination within the temperature range $5{\sim}40^{\circ}C$ varied from 8% to 65%. The highest germination occurred at $15^{\circ}C$. In seeds exposed to salt concentrations in the range 0~400 mM, the highest germination occurred at 0 mM, and the germination progressively decreased as the salt concentration increased. When the perianth of the seed was removed, the germination increased significantly. In the presence or absence of light, germination under white light was 5~20% higher than that under darkness, which varied according to salt concentrations in the range 0~300 mM. The plant growth of S. asparagoides, evaluated by plant height, branch number, root length, fresh weight, and dry weight, was higher in the concentration range 50~100 mM, and it decreased in the other concentrations. In conclusion, the seeds of S. asparagoides showed higher germination in fresh water at low temperature of approximately $15^{\circ}C$ under light when the perianth was removed, and the plant growth was higher at salt concentrations of 50~100 mM.

Blue Mold on Melon (Cucumis melo) Caused by Penicillium oxalicum (Penicillium oxalicum에 의한 멜론 푸른곰팡이병)

  • Wwon, Jin-Hyeuk;Kang, Soo-Woong;Kim, Jung-Soo;Park, Chang-seuk
    • Research in Plant Disease
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    • v.8 no.4
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    • pp.220-223
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    • 2002
  • In April of 2002, fruit rot infected with blue mold was found at maturing stage of melon (Cucumis melo cv. Gayabaegja) growing under tunnel cultivation in Daesan-myon, Haman-gun, Gyeongnam Province, Korea. Floral parts were infected first and colonized by fungal mycelial mats. From the point of infection, fruits become collapsed and mostly ruptured. The pathogenic fungus from infected fruits was isolated. Colony color of the fungus was white on MEA and CYA agar, Conidia were ellipsoid and 2.6~7.4$\times$2.6~5.8 ${\mu}{\textrm}{m}$ in size. Stipes were 86~320$\times$2.8~4.3 ${\mu}{\textrm}{m}$ in size. Metulae were 12.4~31.6$\times$2.6~4.2 ${\mu}{\textrm}{m}$ in size. Phialides were ampulliform to cylindroid, and 8.2~15.4$\times$3.6~4.6 ${\mu}{\textrm}{m}$ in size. Rate of infected fruits in the field was 4.3%. Based on the cultural and mycological characteristics and pathogenecity test on host plants, the fungus was identified as Penicillium oxalicum, This is the first report on the blue mold of melon (Cucumis melo) caused by P. oxalicum in Korea.

Control Effects of Bemisia tabaci on Eggplant using Sticky Trap (가지에서 끈끈이트랩을 이용한 담배가루이 방제효과)

  • Kim, Ju;Choi, In-Young;Lee, Jang-Ho;Kim, Ju-Hee;Lim, Joo-Rag;Cheong, Seong-Soo;Kim, Jin-Ho
    • Korean Journal of Organic Agriculture
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    • v.25 no.4
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    • pp.759-772
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    • 2017
  • This experiment was conducted to develop control method for Bemisia tabaci (Gennadius) on eggplant using sticky trap method. According to the color of the sticky traps, the attractiveness of the B. tabaci was the highest in the yellow trap, followed by the green and orange. However, white, blue, red, black and green sticky traps have reduced attractiveness of B. tabaci. In order to improve the efficiency and attractiveness of sticky trap to the B. tabaci, the different kinds of sugars such as glucose, fructose, oligosaccharide, starch syrup and pure sugar were added to sticky traps respectively. However, the effect of B. tabaci attractiveness was low in starch syrup, pure sugar, and non-treated sticky traps. The attracting effect of B. tabaci was depending on the location of sticky trap. The highest value was obtained where sticky traps were located in the top of the eggplant, followed by 30 cm above from the top level. In addition, we were installed up to 40 sticky traps to determine the optimal amount of sticky traps to control B. tabaci in eggplant. When increasing the sticky traps, the number of adult and nymphs of B. tabaci were tended to be decreased significantly. This tendency was more effective in the latter stages than in the early stages. As the number of sticky traps increased, not only the growth rate of eggplant, leaf length, and stem diameter were to be better. But also number of fruits and product marketable value were increased at the early stage of growing as well. The study had proven that the sticky traps had an effect on increasing the yield at the early stage of growth, but the efficiency of controlling decreased due to the high density of B. tabaci of the next generation.

Quality and Digestibility Characteristics of Rice Cake with Germinated Brown Waxy Rice (발아찰현미떡의 품질 및 소화특성)

  • Kim, Min-Ji;Lee, Su-Jin;Choi, Young-Hee;Son, Dong-Hwa;Chung, Hyun-Jung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.45 no.9
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    • pp.1310-1315
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    • 2016
  • Germinated brown rice (GBR) has received great attention due to its enhanced nutritional value. Brown waxy rice was germinated at $30^{\circ}C$ for 10 h, 20 h, or 30 h with 95% humidity. The color, texture, digestibility, and sensory characteristics of waxy rice cake made by GBR were evaluated. Pasting viscosity of rice flour was substantially reduced, whereas ${\gamma}$-amino butyric acid content increased with an increase in germination time. Lightness of rice cake with GBR was lower than that with brown rice, whereas the redness and yellowness of rice cake were higher after germination. For textural properties, the hardness, gumminess, and chewiness of rice cake with GBR were lower than those with brown rice, and decreased hardness was more prominent with an increase in germination time. The in vitro starch digestibility of rice cake was elevated by germination, showing an increase in rapidly digestible starch content and decrease in resistant starch content. In the sensory evaluation, the overall acceptability of rice cake with brown rice and GBR was greater than that with white rice, whereas it was reduced with an increase in germination time.

Quality Characteristics of Bread with Burdock (Arctium lappa L.) Powder (우엉 분말을 첨가한 식빵의 품질 특성)

  • Tae, Mi Hwa;Kim, Kyoung-Hee;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.12
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    • pp.1826-1831
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    • 2015
  • The study investigated the quality characteristics of white breads prepared 0, 5, 10, and 15% burdock (Arctium lappa L.) powder. Density of the dough was reduced with increasing burdock powder content, whereas pH of the dough increased with increasing content of burdock powder. The baking loss rate of bread decreased as the amount of burdock powder increased. As powder concentration increased, L and a values of crust and L value of crumb decreased, whereas a and b values of crumb increased. The hardness and chewiness of breads increased upon addition of burdock powder, whereas fracturability, cohesiveness, and springiness decreased. 2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity and total polyphenol contents were significantly elevated by addition of burdock powder (P<0.05). In a sensory evaluation, color and flavor were highest in the control group while sweet taste, savory taste, moistness, and chewiness were lowest. The overall acceptability of bread added with 5% burdock powder was higher than both the control and other samples.

Quality Characteristics and Antioxidant Properties of Yanggaeng Supplemented with Hallabong Powder (한라봉 분말을 첨가한 양갱의 품질 및 항산화 활성)

  • Kim, Hyun Eun;Lim, Jeong Ah;Lee, Jun Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.12
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    • pp.1918-1922
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    • 2015
  • The feasibility of incorporating Hallabong powder (HP) as a value-added food ingredient into convenient food products was investigated using yanggaeng as a model system. HP was incorporated into yanggaeng at amounts of 0, 2, 4, 6, and 8% (w/w) based on total weight of cooked white beans and HP. pH level decreased while soluble solid content increased significantly with increasing levels of HP (P<0.05), whereas moisture content was not directly affected by level of HP incorporation. In terms of color, lightness decreased while redness and yellowness increased significantly (P<0.05) with increasing levels of HP. Hardness increased significantly when HP content exceeded 4% in the formulation (P<0.05). ABTS and DPPH radical scavenging activities were significantly elevated by HP addition, and they increased significantly as HP concentration increased in the formulation (P<0.05). Finally, consumer acceptance test indicated that the highest levels of HP incorporation (8%) had an adverse effect on general consumer preferences. In contrast, yanggaeng with moderate levels of HP (2%) is recommended based on its overall scores to take advantage of the antioxidant properties of HP without sacrificing consumer acceptability.