• Title/Summary/Keyword: wheat(Triticum aestivum L.)

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Hot Water Extract of Triticum aestivum L. (Common Wheat) Ameliorates Renal Injury by Inhibiting Apoptosis in a Rat Model of Ischemia/Reperfusion

  • Baek, Hae Sook;Lim, Sun Ha;Ahn, Ki Sung;Lee, Jong Won
    • The Korea Journal of Herbology
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    • v.28 no.3
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    • pp.7-15
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    • 2013
  • Objectives : Interruption and subsequent restoration of blood flow into the kidney result in renal injury. As an approach to preventing the renal injury, we determined the optimal conditions and the underlying mechanisms by which supernatant of hot water extract of ground Triticum aestivum L. (extract) attenuated ischemia/reperfusion (I/R) injury. Methods : One hour after administration of the extract (400 mg/kg) by intraperitoneal injection, renal I/R injury was generated by clamping the left renal artery in rats after surgical removal of the right kidney, followed by reperfusion. The maximal difference between the vehicle-treated and the extract-treated group under ketamine/xylazine or enflurane anesthetization was assessed at varying periods of ischemia (30-45 min) and reperfusion (3-48 hr), based on the renal function assessed with serum creatinine levels, tissue injury with hematoxylin/eosin staining, and apoptosis with terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling staining. Results : Enflurane anesthetization with 40 min of ischemia and 24 hr of reperfusion was identified to be the optimal condition, under which condition serum creatinine levels and tubular damage in the extract-treated group were significantly reduced compared with those in the vehicle-treated group ($1.3{\pm}0.2$ versus $2.7{\pm}0.3$ mg/dL, P < 0.01, and average score $1.8{\pm}0.1$ versus $3.5{\pm}0.3$, P < 0.01, respectively). These beneficial effects were mediated by inhibition of apoptotic cascades through attenuation of renal tissue malondialdehyde levels, Bax/Bcl-2 ratio and caspase-3 levels. Conclusions : The extract conferred renal protection against ischemia/reperfusion injury in rats by scavenging reactive oxygen species and consequently blocking apoptotic cascades, plausibly augmented by enflurane protection.

Effects of Temperature and Daylength on Growth and Grain Yield in Wheat (T. aestivum) (온도 및 일장조건이 소맥의 생육 및 수량에 미치는 영향)

  • Cho, C.H.;Chung, T.Y.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.24 no.2
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    • pp.35-41
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    • 1979
  • To observe the effects of temperature and photoperiod on the growth and yield of winter wheat (Triticum aestivum, L) eight varieties including Chang Kwang were tested at various treatments like longday-high temperature (24hrs-20\circ), shortdayhigh temperature (12hrs-20\circ), longday-low temperature (24hrs-14\circ) and shortday-low temperature (12hrs-14\circ). Among the traits measured, days to heading, plant height, spike length, number of spikes per hill and grain yield per hill were generally decreased at high temperature and long day treatment and increased at low temperature and shortday condition. Number of grains per spike was decreased at low temperature and short day condition while increased at longday and high temperature conditions. Grain weight was decreased significantly at high temperature and shortday while increased at low temperature and longday treatment.

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Proteomics Approach on Puroindoline Gene of Pre-harvest Sprouting Wheat

  • Kamal, Abu Hena Mostafa;Park, Cheol-Soo;Heo, Hwa-Young;Chung, Keun-Yook;Cho, Yong-Gu;Kim, Hong-Sig;Song, Beom-Heon;Lee, Chul-Won;Woo, Sun-Hee
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.205-212
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    • 2009
  • Wheat (Triticum aestivum L.) grain texture is an important determinant of milling properties and end product use. Two linked genes, puroindoline a (PINA) and puroindoline b (PINB), control most of the genetic variation in wheat grain texture. Wheat seed proteins were examined to identify PINA and PINB gene using two pre-harvest sprouting wheat cultivars; Jinpum (resistant) and Keumgang (susceptible).Wheat seed proteins were separated by two-dimensional electrophoresis with IEF gels over pH ranges: pH 3-10. A total of 73 spots were digested with trypsin resulting peptide fragmentation were analyzed by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/MS). Mass spectra were automatically processed and searched through NCBInr, SWISS-PORT and MSDB database with mono isotopic masses and complete gene sequence were found by UniProt database. Puroindoline a and puroindoline b that is responsible for grain texture related with baking performance and roughness. Two spots were found Pin b (16.7 kDa) and Pin a (16.3 kDa) in Jinpum compare to seven spots were identified Pin a (16.1 kDa, 16.3 kDa) and Pin b (16.7 kDa, 9.5 kDa and 14.4 kDa) in Keumgang. Some selected spots were identified puroindoline like grain softness protein (16.9 kDa, 17 kDa and 18.1 kDa) in Keumgang. Moreover, to gain a better inferring the identification of puroindoline related proteins using proteomics, we accomplished a complete gene sequence of PINA and PINB gene in pre-harvesting sprouting wheat seeds between resistant (Jinpum) and susceptible (Keumgang).

Purification and Properties of Wheat Fructan Exohydrolase (밀 Frucdtan Exohydrolase의 분리 및 특성)

  • Byeong Ryong, Jeong;Thomas L, Housley
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.456-464
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    • 1996
  • Fructans are the major vegetative storage carbohydrate in wheat(Triticum aestivum L.). The depolymerization of fructans occurs by the sequential removal of terminal fructosyl residues by a specific fructan exohydrolase(FEH). The objective of this study was to isolate and characterize this enzyme in wheat. From stems and sheaths of field-grown wheat(cv. Clark), FEH was purified 356-fold using salt precipitation and a series of chromatographic procedures including size exclusion, anion exchange, and affinity chromatography. FEH had a molecular weight of 63.7 kD and an optima at pH 5.5 and 3$0^{\circ}C$. The $K_{m}$s for $\beta$(2 longrightarrow1) linked oligofructans varied, from 10 to 37mM, with the lowest $K_{m}$ for tetrasaccharide. The $V_{max}$ increased as degree of polymerization (DP) increased. Wheat FEH hydrolyzed only, $\beta$(2 longrightarrow1) linked fructans but not, $\beta$(2 longrightarrow6) linked timothy fructan or sucrose. The role of this FEH in fructan metabolism in wheat is discussed.sed.

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Physicochemical Properties and Sugar-Snap Cookie Making Potentialities of Soft Wheat Cultivars and Lines (Triticum aestivum L. em Thell.) (연질밀(Triticum aestivum L. em Thell.)의 이화학적 특성과 sugar-snap cookie의 제조적성)

  • Lee, Yong-Suk;Kim, Jong-Goon;Won, Joon-Hyung;Chang, Hak-Gil
    • Korean Journal of Food Science and Technology
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    • v.34 no.5
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    • pp.846-855
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    • 2002
  • Several soft white spring and winter wheat cultivars were evaluated by analyzing physicochemical properties such as Single Kernel Characterization System (SKCS), milling properties, Rapid Visco-Analyzer (RVA), mixograph pattern, and sugar-snap cookie-making potentialities. Results of SKCS revealed kernel hardness had a positive correlation coefficient with test weight $(r=0.497^{*})$ and near-infrared reflectance (NIR) hardness $(r=0.495^{*})$. SKCS kernel weight had a significantly high correlation of $r=0.942^{**}$ with SKCS kernel size. The test weight had significantly high correlations with straight-grade flour yield $(r=0.720^{**})$, break flour yield $(r=0.490^{*})$, flour ash content $(r=-0.781^{**})$, and milling score $(r=0.838^{**})$. The average RVA peak viscosity of the soft white winter wheat was higher (195.1 unit) than the soft white winter wheat varieties (135.7 unit). A correlation was found between RVA peak viscosity and swelling volume. Significant correlation coefficients were obtained among cookie properties, milling properties, protein content, and mixograph absorption. The cookie top grain score had a correlation coefficient of $r=0.447^{*}$ with swelling volume.