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Nutrient Utilization, Body Composition and Lactation Performance of First Lactation Bali Cows (Bos sondaicus) on Grass-Legume Based Diets

  • Sukarini, I.A.M.;Sastradipradja, D.;Sutardi, T.;Mahardika, IG.;Budiarta, IG.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.12
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    • pp.1681-1690
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    • 2000
  • A study on energy and protein utilization, and milk production of Bali cows on grass-legume diets was carried out using 12 first lactation cows (initial BW $263.79{\pm}21.66kg$) during a period of 16 weeks starting immediately post calving. The animals were randomly allotted into 4 dietary treatment groups R1, R2, R3 and R4, receiving from the last 2 months of pregnancy onwards, graded improved rations based on a mixture of locally available grass and legume feed ad libitum. R1 contained on a DM basis 70% elephant grass (PP, Penisetum purpureum) plus 30% Gliricidia sepia leaves (GS), R2 was 30% PP plus 55% GS supplemented with 15% Hibiscus tilliactus leaves (HT, defaunating effect), R3 and R4 were 22.5% PP+41.25% GS+11.25% HT+25% concentrate, where R3 was not and R4 supplemented with zinc di-acetate. TDN, CP and zinc contents of the diets were 58.2%, 12.05% and 18.3 mg/kg respectively for R1, 65.05%, 16.9% and 25.6 mg/kg respectively for R2, 66.03%, 16.71% and 29.02 mg/kg respectively for R3 and 66.03%, 16.71% and 60.47 mg/kg respectively for R4. Milk production and body weight were monitored throughout the experimental period. In vivo body composition by the urea space technique validated by the body density method and supported by carcass data was estimated at the start and termination of the experiment. Nutrient balance and rumen performance characteristics were measured during a balance trial of 7 days during the 3rd and 4th week of the lactation period. Results indicated that quality of ration caused improvement of ruminal total VFA concentration, increments being 52 to 65% for R2, R3 and R4 above R1, with increments of acetate being less (31 to 48%) and propionate being proportionally more in comparison to total VFA increments. Similarly, ammonia concentrations increased to 5.24 to 7.07 mM, equivalent to 7.34 to 9.90 mg $NH_3-N/100ml$ rumen fluid. Results also indicated that feed quality did not affect DE and ME intakes, and heat production (HP), but increased GE, UE, energy in milk and total retained energy (RE total) in body tissues and milk. Intake-, digestible- and catabolized-protein, and retained-protein in body tissues and milk (Rprot) were all elevated increasing the quality of ration. Similar results were obtained for milk yield and components with mean values reaching 2.085 kg/d (R4) versus 0.92 kg/d (R1) for milk yield, and 170.22 g/d (R4) vs 71.69 g/d (R1), 105.74 g/d (R4) vs 45.35 g/d (R1), 101.34 g/d (R4) vs 46.36 g/d (R1) for milk-fat, -protein, and -lactose, respectively. Relatively high yields of milk production was maintained longer for R4 as compared to the other treatment groups. There were no significant effects on body mass and components due to lactation. From the relationship $RE_{total}$ (MJ/d)=12.79-0.373 ME (MJ/d); (r=0.73), it was found that $ME_{m}=0.53MJ/kgW^{0.75}.d$. Requirement of energy to support the production of milk, ranging from 0.5 to 3.0 kg/d, follows the equation: Milk Prod. ($Q_{mp}$, kg/d)=[-2.48+4.31 ME($MJ/kg^{0.75}.d$)]; (r=0.6) or $Q_{mp}$=-3.4+[0.08($ME-RE_{body\;tissue}$)]MJ/d]; (r=0.94). The requirement for protein intake for maintenance ($IP_m$) equals $6.19 g/kg^{0.75}.d$ derived from the relationship RP=-47.4+0.12 IP; (r=0.74, n=9). Equation for protein requirement for lactation is $Q_{nl}$=[($Q_{mp}$)(% protein in milk)($I_{mp}$)]/100, where $Q_{nl}$ is g protein required for lactation, $Q_{mp}$ is daily milk yield, Bali cow's milk-protein content av. 5.04%, and $I_{mp}$ is metabolic increment for milk production ($ME_{lakt}/ME_{m}=1.46$).

Studies on Energy Metabolism of Growing Chicken (닭의 에너지 대사(代謝)에 관(關)한 연구(硏究))

  • Kwon, Soon Ki;Oh, Hong Rock
    • Korean Journal of Agricultural Science
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    • v.9 no.2
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    • pp.540-545
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    • 1982
  • An energy metabolism study was conducted with two commercial strains of meat-type chickens, Hubbard and Cobb, and two egg strains, Hy Line and Korean-bred Hanhyup 325. The heat production of growing chickens from each strain were measured by the use of an open-circuit gravimetric respiration calorimeter. The data obtained from this study were summarized as fallows. 1. The average body weight of 9-wk-old Hubbard broilers reared in battery cages was 2,570g/bird. The average body weights of 9-wk-old Hy Line chicks and Hanhyup 325 were 777 and 748g/bird, respectively. 2. At 3 weeks of age, the Hubbard broiler chicks consumed two times the feed consumed by Hy Line chicks (54.6g VS. 26.7g/bird/day). These values increased to 151g and 57.2g/bird/day, respectively, at 8 weeks of age, indicating that the difference in feed intake between meat and egg-type chicks tends to increase as they grow older. In terms of water consumption, the 5-wk-old Hubbard broiler chicks drank $226m{\ell}/bird/day$ as compared with $58m{\ell}$ by Hy Line chicks. These values increased to 282 and $70m{\ell}$, respectively, at 8 weeks of age. 3. The excreta outputs of Hubbard broilers and Hy Line chicks were 18.7 and 6.1g DM/bird/day at 4 weeks of age, and 41.5 and 10.0g DM/bird/day at 8 weeks of age, respectively. 4. The energy metabolizability of broiler chicks were 75.4~77.1% compared to 75.0~83.5% by egg-type chicks. 5. The respiratory quotient (RQ) was between 0.78 and 0.97. There seems to be no difference in RQ between meat and egg-type chicks. The RQ tended to decrease when feed intake was low and vice versa. 6. Both meat and egg-type chicks produced $83.1{\sim}123.1Kcal/kg^{\frac{3}{4}}B.W./day$. The considerably low value of $83Kcal/kg^{\frac{3}{4}}B.W./day$ was obtained when the chicks were off the feed under the stressful conditions. The high value of 123.1Kcal was obtained when the chicken chamber temperature rose to $27{\sim}34^{\circ}C$.

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Mapping of the Damaged Forest by Oak Wilt Disease in Bukhansan National Park (북한산국립공원 참나무시들음병 피해지 맵핑 연구)

  • Yeum, Jung-Hun;Han, Bong-Ho;Choi, Jin-Woo;Jeong, Hee-Un
    • Korean Journal of Environment and Ecology
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    • v.27 no.6
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    • pp.704-717
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    • 2013
  • This study aims to provide basic data for management and prevention of infection damage by Oak wilt disease through mapping method of status with infected level in damaged area of Bukhansan National Park. Survey was carried out in the distributed area of oak trees with mapping unit of polygon of actual vegetation and mapped of infection ratio and infection index applying weight according to infected level. Infection ratio of oak trees in Bukhansan National Park was 58.5%, and lightly damaged ratio was 29.6%, half of the damaged ratio was 16.1%, seriously damaged ratio was 8.8% and withered ratio was 4.1%. It was serious to be infected from Beomgol ridge in Wondobong district to Hyeongjaebong in Jeongrung district. Although the infected ratio of the western part of Songchu district, Sanseong district and Gugi district centering main ridge of Bukhansan National Park was low, it of ridge and main valley was high. Infection index of hardly damaged area was 39.1% of whole area, and slightly damaged area was 41.0%, half of the damaged area was 16.1%, seriously damaged area was 3.3% and alarmed withering area was 0.4%. Infection index was high around Musugol valley in Dobong district and Jaunbong, and it of Bohyunbong of Jeongrung district and the part of Hyojari valley of Sanseong district was serious. Predicted numbers of the trees affected Oak wilt disease compared to the distributed area of oak trees was 1,585,937 trees among 2,709,147 trees of Quercus spp. 352,931 trees among the 306,161 trees of oak were infected in Woi district, the most seriously infected area and 53,141 trees among the 145,747 trees of oak was infected in Gugi district, the most slightly infected area.

Effective Tillering Pattern and Grain Yield on Different Sowing Depth in Barley (보리 파종심도에 따른 유효분벽의 양상과 수량)

  • 신만균
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.6
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    • pp.671-683
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    • 1995
  • This study was aimed to provide understanding on the eco-physiological response of barley tillers as affected by sowing depth. Yield and yield contribution rate of tillers were investigated with the data of field experiments in the former Wheat and Barley Research Institute of Suwon, Korea from October 1983 to July 1984. When barley was sowed in various depth of 1, 3, 5, 7 and 9cm below the ground surface, 13 mainstem leaves appeared by 3 and 5cm treatments, and 11 leaves by 1, 7 and 9cm treatments. The effective tillers were observed from 8/0 in lcm depth, while 10/0 in 3-5cm depth and 9/0 in 7cm depth. There was no coleoptile tiller in 7cm depth sowing. Deep and shallow sowings produced fewer leaves and tillers, as early growth was hindered by deep sowing whereas cold damage was apparent in shallow sowing. Accordingly, more effective tillers per plant and higher grain weight per ear were observed in 3∼5cm depth sowing. Yield contribution by the tillers with various sowing depth was as follows: mainstem, 1, 2, 11, 3, 21, 4 and 12. The contribution of 1P, 13, 2P, 23 and 31 varied with the treatments.

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Measurement of Leaf Area of Soybeans (대두엽면적의 간이측정법)

  • Kwon-Yawl Chang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.93-95
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    • 1968
  • The importance of leaf area as related to transpiration and photosynthesis is generally recognized. In general, a compound leaf of soybean consist of one main leaflet and two side leaflets from each node of the stem. Takahashi and Fukuyama (1919) classified soybeans into three types, namely the long leaf type, round leaf type, and intermediate type, in which the last one had round leaves at the base and long leaves in the upper part of the stem. Nagai (1925) and Takahashi (1935). dealt with the genetics of the leaf form and association with other characters. The closely relationships, the correlation coefficients from 0.64 to 0.73, were shown between the leaf area and the soybean yield in the experiments by Nagai (1942). Nagata (1950) also tested the varietal differences of the variation of leaf length and its ratio to the leaf width on the nodes of stem, and finally divided varieties into five types. Three methods of measuring area of strawberry leaves were used by Darrow (1932). The first involved determining a factor to be used with length or length ${\times}$width measurements. The second method involved placing leaves on pieces of cardboard of known area cut to the shape of the leaves. Direct use of the planimeter on intact leaves was Darrow's third method. Miller (1938) enumerated several methods to determine the leaf surface area in plants, some of which were extremely laborious and required removing leaves from plants. They included tracing outlines of leaves on paper and measuring the enclosed area with a planimeter or cutting out the traced areas and comparing the weights obtained with the weight of a known paper. Another method involved placing the form of the leaf on sensitized paper with the area being determined by measuring or weighing as above. Miller further stated that the photoelectric cell can also be utilized to estitmate leaf area. Working with field beans, Davis (1940) found that 0.004517 (length ${\times}$ width) of the center leaflet was the most nearly accurate of four methods attempted. A simple procedure to measure leaf area in corn was devised 1 y Montgomery (1911) and used by Kiesselbach (1950). The formula was length ${\times}$ width ${\times}$ 0.75. Stickler et al. (1961) have successfully used length times width ${\times}$ 0.747 to estimate area of grain sorghum leaves. Bhan and Pande(1966) has also used length ${\times}$ width ${\times}$ 0.802 to determine leaf area of rice varieties. The main objectives of the present investigation were to develop an accurate, rapid method to determine leaf area in soybean varieties and to examine certain data associated with leaf area determinations.

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Multivariate Analysis of Variation of Growth and Quality Characteristics in Colored Rice Germplasm (유색미 도입 유전자원의 생육 및 품질특성 변이 다변량 분석)

  • Park, Jong-Hyun;Lee, Ji-Yoon;Chun, Jae-Buhm;You, Oh-Jong;Son, Eun-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.63 no.3
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    • pp.175-185
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    • 2018
  • The aim of this study was to evaluate the variation of growth and quality characteristics in colored rice from 178 accessions and to develop useful, basic rice breeding data by classifying these germplasm characteristics via principal component (PC) analysis. The coefficient of variation of the 178colored rice accessions were the highest for panicle length (PL) and protein contents, followed by length-width ratio (LWR), 1000-grain weight (TGW), culm length (CL), and amylose contents, whereas the lowest was for the number of panicles per hill (NP), which is a yield component. The results from the PC analysis exhibited eigenvalues and contributions respective to each PC as follows: PC1, 2.06 and 29.49%; PC2, 1.31 and 18.75%; PC3, 1.21 and 17.36%; PC4, 1.01 and 14.38%. The eigenvalues of four PCs were over 1.0, and their cumulative contributions were 79.98%, which completes the necessary condition for evaluation of the 178 colored rice accessions. Cluster analysis showed cluster I as the largest, which included 79 accessions, while clusters II, III, IV, V, VI, and VII comprised 46, 19, 13, 4, 8, and 9 accessions, respectively. Moreover, dark brown accessions were dispersed in clusters I and II, and many resources of purple seed coat color were found in clusters V, VI, and VII. Particularly, cluster V had resources of only black and purple seed coat colors. Resources of cluster VII were found to have a relatively small average CL, PL, and LWR; notably, cluster V had the smallest average TGW, and cluster IV the lowest NP but the highest TGW. Finally, considering the yield potential, growth characteristics, heading stage, and color during breeding of colored rice, we obtained the following conclusions: cluster VII is suitable for breeding of colored rice; cross breeding among clusters I, II, and VII has a high yield potential; and it is possible to produce a superior color by cross breeding plants from cluster V and VI.

Studies on the Effects of Economic Traits on the Frequency of Soft and Broken Eggs in Laying Hens 1. Correlation between Economic Traits on the Frequency of Soft and Broken Eggs in Colored Laying Hens (산란계의 경제형질과 연파란 발생비율에 관한 연구 1. 유색산란계 연파란율과 다른 형질과의 상관)

  • 정익정;송인섭;정선부
    • Korean Journal of Poultry Science
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    • v.19 no.4
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    • pp.177-185
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    • 1992
  • This study was carried out to investigate the relationship between frequency of soft and broken eggs and the economic traits for colored laying hens using the data sampled 7 times in Korea from 1985 to 1991. In addition, the correlation coefficients of frequency of soft and broken eggs on the economic traits for colored laying hens in order to decrease the frequency of soft and broken eggs were studied. The results obtained were summarized as follows : 1. For colored laying hens, mean of the sexual maturity(SM) was 163.6 days, hen-day egg Production(HDEP) was 79.49%, hen-housed egg Production(HHEP) was 272.9 eggs, egg weight (EW) was 62.69g and the frequency of soft and broken eggs was 1.363% during 18∼72wks. For white laying hens, SM was 157.2 days, HDEP was 77.87%, HHEP was 265.1 eggs, EW was 60. 26g and the frequency of soft and broken eggs (FSBE) was 1.174% during 18∼72wks. 2. Statistical significances were observed in laying viability, SM, HDEP, FR, BW and FSBE during 18 ∼72wks and 18 ∼78wks by year for colored laying hens. 3. Sexual maturity was shortened from 168.5 days to 155.9 days. FSBE decreased from 1.4∼l.69% to 0.94∼o.89% during 18∼72wks, and HDEP was increased from 74.5∼75.1% to 77.8∼80.6%, HHEP was increased to 302.0∼304.6 eggs and FSBE was increased from 1.54∼2.02% to 0.98% during 18∼78wks in colored laying hens. 4. Estimate of correlation coefficients between FSBE and trait of SM, EW, 72 wks BW were 0.3985, 0.1072, 0.4225, and those of HDEP, HHEP, FR were -0.5662, -2896, -0.245 during 18∼72wks In colored laying hens, respectively. 5. Estimate of correlation coefficients between FSBE and traits of SM, EW, 78wks BW were 0.3791, 0.1171, 0.4609, and those of HDEP, HHEP, FR were -0.5041, -0.2372, -0.2421 during 18∼78wks in colored laying hens, respectively.

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Clinical Features of Cricopharyngeal Incoordination in Newborns and Infants (신생아 및 영아기 윤상인두 협조불능의 임상적 고찰)

  • Han, Young-Mi;Park, Jae-Hong
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.11 no.2
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    • pp.116-121
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    • 2008
  • Purpose: Cricopharyngeal incoordination is a rare cause of swallowing difficulties in newborns and infants; it is characterized by delayed pharyngeal contractions related to cricopharyngeal relaxation. Dysphagia and repeated aspiration are common findings despite normal sucking. We conducted this study to assess the clinical features of cricopharyngeal incoordination in newborns and infants. Methods: An analysis of the clinical data from 17 patients with cricopharyngeal incoordination who were admitted to the Department of Pediatrics, Pusan National University Hospital, between 2000 and 2006 was conducted retrospectively. The diagnosis of cricopharyngeal incoordination was established by the clinical characteristics and the videofluoroscopic swallowing studies. Results: The male to female ratio was 1:1.1 (males 8, females 9) the age range 1 to 60 days. The body weight of 11 patients (64.7%) was less than the $10^{th}$ percentile at diagnosis. Six patients (35.3%) were born prematurely. The associated anomalies or diseases were chromosomal anomaly (2 cases), congenital heart disease (3 cases), and laryngomalacia, hypoxic brain damage or neonatal seizures (1 case each). The chief complaints of patients were recurrent aspiration pneumonia (10 cases), feeding difficulty (9 cases), dyspnea (4 cases), and chocking (4 cases). The severity of aspiration on the videoesophagogram or esophagogram was mild in 12 cases. The correlation between the severity of aspiration and the duration of tube feeding after the diagnosis was significant (p<0.05). Conclusion: Cricopharyngeal incoordination should be considered in the differential diagnosis of newborns and infants, without known risk factors associated with swallowing dysfunction, when they present with unexplained respiratory problems. Although the prognosis of cricopharyngeal incoordination is good, early diagnosis and tube feeding are recommended to prevent the complications associated with this disorder.

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Development of animal protein(feed for fry) utilizing the rumen ciliates (제1위 섬모충(rumen ciliates)을 이용한 동물성 단백질(치어용 사료) 개발)

  • Jee, Cha-ho;Hyun, Gong-yool
    • Korean Journal of Veterinary Research
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    • v.35 no.2
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    • pp.327-336
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    • 1995
  • This study was carried out to develop the animal protein(feed for fry) that was isolated, purified and lyophilized the rumen ciliates from the healthy rumen contents which have $10^5-10^6/g$ ciliates and were discarded in abattoirs. The rumen ciliates are non-pathogenic, anaerobic and the weight of this protozoa is 2% of rumen content. The rumen protozoan and bacterial proteins both have a biological value for rats of 80-81, which is higher than the 72 of brewer's yeasts. Furthermore, the true digestibility and net protein utility of the protozoan protein are 91 and 73, much higher than those of bacterial(74 and 60) or yeast(84 and 60) proteins. The amino acids of rumen protozoa is nutritionally superior than the others. The size of rumen ciliates is $30-200{\times}20-110{\mu}m$ and so we had isolated and purified the rumen ciliates from the rumen contents by the physical methods. The purified rumen protozoa was lyophilized with freezing dryer. The results of this experiment were as follows : 1. Population dynamics of protozoan ciliates in slaughtered rumens; % of samples which small ciliates were predominated was 82.5%(52/63) and that of large ciliates was 17.5%(11/63). 1) predominant species of small ciliates were Entodinium ovinum and E nanellum. 2) predominant species of large ciliates were Epidinium ecaudatum and Diploplastron affine. 2. The lyophilized rumen ciliates which were isolated and purified from 1 kg of rumen content at the pH 6.2-6.8 was about 7.0 gram. 3. The nutrient analysis of lyophilized rqmen ciliates(LRC) was as follows: 1) Proximate analysis of the LRC and the composition of fry feed; moisture 8.05%(below 10.0), protein 35.37%(45), fat 5.39%(4.5), fiber 1.23%(below 2.5), ash 2.25%(below 15.0), Ca 0.26%(below 2.0), P 0.14%(below 1.1), energy 4,608.11(fish meal 5000 cal/g) 2) Amino acids (% in crude protein) of the LRC and the rotifer(Brachionus plicatilis); Arg 5.19%(4.50), His 2.50%(1.55), Ile 5.29%(3.45), Leu 8.11%(5.85), Lys 10.34%(6.15), Met 2.25% (0.85), Phe 5.66%(3.80), Thr 5.14% (3.45), Val 4.18%(3.90), Ala 4.13%(3.35), Asp 13.26%(8.25), Glu 16.62%(9.20), Gly 4.23%(3.10), Pro 3.25%(5.05), Ser 4.85%(3.85), Tyr 5.04%(3.05) 3) Fatty acids(% in fat) of the LRC and the rotifer(biological feed ; Brachionus plicatilis); myristic acid(C14:0) 3.27%(0.3), myristoleic acid(C14:1) 0.83%(-), palmitic acid(C16:0) 39.11% (23.5), palmitoleic acid(C16:1) 2.81%(2.0), stearic acid(C18:0) 9.36%(5.6), oleic acid(C18:1) 25.54%(3.5), linoleic acid(C18:2) 15.05%(32.9), linolenic acid(C18:3) 1.74%(9.8). Judging from the above investigated results, the analytical data of proximate analysis, amino acids, fatty acids of the purified and lyophilized rumen protozoa are reasonable for the feed of freshwater fishes(fry and fingerling). But it was disappointed of our expectation that the crude protein of lyophilized rumen ciliates contains low percentage, it was thought that because of the small ciliates(starch digester) in beef cattle rumens which were administered the concentrated feed, is much difficult to isolate and purify than the large ciliates(fiber digester).

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Surgical Treatment of Primary Lung Cancer (원발성 폐암의 외과적 치료)

  • 김성완;구본원;이응배;전상훈;장봉현;이종태;김규태;강덕식
    • Journal of Chest Surgery
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    • v.31 no.2
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    • pp.134-141
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    • 1998
  • Primary lung cancer has recently increased progressively in its incidence in Korea. It is clearly evident that surgical resection offers the best offortunity for cure of non-small cell carcinoma. This study was designed to analyse the clinical data of 100 primary non-small cell carcinoma patients who underwent lung resection surgery from January 1992 to July 1995 at the department of Thoracic and Cardiovascular Sugery, Kyungpook National University Hospital. There were 86 males and 14 females(6:1). In the age distribution, the peak incidence was recorded in the seventh decade(43%). The methods of tissue diagnosis were bronchoscopic biopsy in 53 patients(50.5%), percutaneous needle aspiration in 17 patients(16.2%), transbronchial lung biopsy in 11 patients(10.5%), mediastinoscopic biopsy in 2 patients (1.9%), sputum cytology in 2 patients(1.9%), and thoracotomy in 20 patients(19.0%). Fifty-five lobectomies, 22 pneumonectomies, 15 bilobectomies, 2 segmentectomies, 4 sleeve lobectomies, a sleeve pneumonectomy, and a wedge pneumonectomy were performed. Operative mortality occured in 4 cases(sepsis in 2 cases, respiratory failure in 1 case, and acute myocardiac infarction in 1 case). The histologic types of tumor were 67 squamous cell carcinomas, 26 adenocarcinomas, 6 large cell carcinomas, and an adenosquamous cell carcinoma. Eighteen patients with N2 mediastinal lymph node metastases had 8 squamous cell carcinomas(11.9%), 9 adenocarcinomas(34.6%), and a large cell carcinoma(16.7%). The primary tumors in these patients were in the right upper lobe in 4 patients, the right middle and lower lobe in 9 patients, the left upper lobe in 3 patients, and the left lower lobe in 2 patients. With regard to pathologic stages, 45 patients had stage I disease; 13 patients, stage II; 36 patients, stage IIIa; 5 patients, stage IIIb; and 1 patient, stage IV. The overall actuarial survival rate was 77.5% at 12 months, 56.1% at 24 months and 43.7% at 43 months. The actuarial survival rates at 43 months were 81.3% in Stage I, 20.8% in Stage II, 27.9% in Stage IIIa, 25.0% in Stage IIIb and 33.3% in Stage IV. These facts suggest that early detection and surgical resection are recommended for favorable postoperative survival in non-small cell lung cancer.

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